Red Blood Cell Aging During Storage, Studied Using Optical Tweezers Experiment

Czerwinska, Justyna; Wolf, Stefan; Mohammadi, Hânieh; Jeney, Sylvia

doi:10.1007/s12195-015-0380-0

Cited by 15 publications

(16 citation statements)

References 23 publications

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“…The elongation index (EI) and deformation index (DI) were phenomenologically defined and are strongly dependent on the flow velocity. Instead of measuring EI or DI, the shear modulus of RBCs was measured using the optical tweezers method to characterize stored RBC deformability changes 49 . Quantification of the laser tweezers applied forces was obtained by comparing the experimental data with finite element simulation.…”

Section: Rbc Effective Stiffness Increases During Storagementioning

confidence: 99%

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Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

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In transfusion medicine, the deformability of stored red blood cells (RBCs) changes during storage in blood banks. Compromised RBC deformability can reduce the transfusion efficiency or intensify transfusion complications, such as sepsis. This paper reports the microfluidic mechanical measurement of stored RBCs under the physiological deformation mode (that is, folding). Instead of using phenomenological metrics of deformation or elongation indices (DI or EI), the effective stiffness of RBCs, a flow velocityindependent parameter, is defined and used for the first time to evaluate the mechanical degradation of RBCs during storage. Fresh RBCs and RBCs stored up to 6 weeks (42 days) in the blood bank were measured, revealing that the effective stiffness of RBCs increases over the storage process. RBCs stored for 1 week started to show significantly higher stiffness than fresh RBCs, and stored RBC stiffness degraded faster during the last 3 weeks than during the first 3 weeks. Furthermore, the results indicate that the time points of the effective stiffness increase coincide well with the degradation patterns of S-nitrosothiols (SNO) and adenosine triphosphate (ATP) in RBC storage lesions.

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Section: Rbc Effective Stiffness Increases During Storagementioning

confidence: 99%

“…Unfortunately, no shear modulus change data were presented beyond 21 days of storage in Ref. 49, although 42 days is specified as the shelf life in most countries. Recently, a deformability-based microfluidic device was reported for sorting stiff and less stiff RBCs 50 .…”

Section: Rbc Effective Stiffness Increases During Storagementioning

confidence: 99%

Stiffness increase of red blood cells during storage

Zheng

Wang

et al. 2018

Microsyst Nanoeng

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“…By evaluating the RBC deformability by OTs, RBC aging during in vitro storage for blood transfusion purposes has been evaluated quantitatively in recent years. It is discovered that the elastic shear modulus of the RBC membrane measured by the OTs-based stretching method increased from 2.5 µN/m to 13 µN/m over 21-day storage at 4°C after donation [137]. The phenomenon of RBC membrane stiffening has been further confirmed and investigated in detail for 15-day storage by evaluating the elongation of the optically trapped RBCs under various dragging velocity against the blood serum [138].…”

Section: Evaluation Of Rbc Membrane Deformationmentioning

confidence: 84%

Optical Tweezers in Studies of Red Blood Cells

Zhu

Avsievich

Попов

et al. 2020

Cells

106

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Optical tweezers (OTs) are innovative instruments utilized for the manipulation of microscopic biological objects of interest. Rapid improvements in precision and degree of freedom of multichannel and multifunctional OTs have ushered in a new era of studies in basic physical and chemical properties of living tissues and unknown biomechanics in biological processes. Nowadays, OTs are used extensively for studying living cells and have initiated far-reaching influence in various fundamental studies in life sciences. There is also a high potential for using OTs in haemorheology, investigations of blood microcirculation and the mutual interplay of blood cells. In fact, in spite of their great promise in the application of OTs-based approaches for the study of blood, cell formation and maturation in erythropoiesis have not been fully explored. In this review, the background of OTs, their state-of-the-art applications in exploring single-cell level characteristics and bio-rheological properties of mature red blood cells (RBCs) as well as the OTs-assisted studies on erythropoiesis are summarized and presented. The advance developments and future perspectives of the OTs’ application in haemorheology both for fundamental and practical in-depth studies of RBCs formation, functional diagnostics and therapeutic needs are highlighted.

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“…Using atomic force microscopy, previous studies have shown a significant increase in Young’s modulus of the cells over the storage time indicating a decrease in the elasticity of the cells 8,9 . In addition, optical tweezers have been used by Czerwinska et al to stretch RBCs and measure a significant increase in shear modulus over storage time, demonstrating a stiffer membrane 10 . A study by Card et al used an ektacytometer to measure a general decrease in deformability of blood during storage by monitoring shape changes using diffractive imaging and a concentric cylinder viscometer 11 .…”

Section: Introductionmentioning

confidence: 99%

Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes

et al. 2019

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Changes in the deformability of red blood cells can reveal a range of pathologies. For example, cells which have been stored for transfusion are known to exhibit progressively impaired deformability. Thus, this aspect of red blood cells has been characterized previously using a range of techniques. In this paper, we show a novel approach for examining the biophysical response of the cells with quantitative phase imaging. Specifically, optical volume changes are observed as the cells transit restrictive channels of a microfluidic chip in a high refractive index medium. The optical volume changes indicate an increase of cell’s internal density, ostensibly due to water displacement. Here, we characterize these changes over time for red blood cells from two subjects. By storage day 29, a significant decrease in the magnitude of optical volume change in response to mechanical stress was witnessed. The exchange of water with the environment due to mechanical stress is seen to modulate with storage time, suggesting a potential means for studying cell storage.

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Red Blood Cell Aging During Storage, Studied Using Optical Tweezers Experiment

Cited by 15 publications

References 23 publications

Stiffness increase of red blood cells during storage

Stiffness increase of red blood cells during storage

Optical Tweezers in Studies of Red Blood Cells

Quantitative phase imaging of erythrocytes under microfluidic constriction in a high refractive index medium reveals water content changes

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