Red blood cell lingering modulates hematocrit distribution in the microcirculation

Rashidi, Yazdan; Simionato, Greta; Zhou, Qi; John, Thomas; Kihm, Alexander; Bendaoud, Mohammed; Bernabéu, Miguel O.; Kaestner, Lars; Laschke, Matthias W.; Menger, Michael D.; Wagner, Christian; Darras, Alexis

doi:10.1016/j.bpj.2023.03.020

Cited by 13 publications

(4 citation statements)

References 48 publications

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“…The animals were maintained on a standard 12/12 h day/night cycle, and water and food were provided ad libitum. The RBC flow in the hamster mesentery was captured using blue light intravital microscopic transillumination (Axio Examiner.D1, Carl Zeiss Microscopy GmbH, Göttingen, Germany), equipped with a high-speed camera (Hamamatsu Orca Flash 4.0, C13440, Hamamatsu Photonics Deutschland GmbH, Herrsching am Ammersee, Germany) [63].…”

Section: In Vivo Rbc Imagingmentioning

confidence: 99%

Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow

Nouaman,

Darras,

John

et al. 2023

Cells

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Blood flow in the microcirculatory system is crucially affected by intrinsic red blood cell (RBC) properties, such as their deformability. In the smallest vessels of this network, RBCs adapt their shapes to the flow conditions. Although it is known that the age of RBCs modifies their physical properties, such as increased cytosol viscosity and altered viscoelastic membrane properties, the evolution of their shape-adapting abilities during senescence remains unclear. In this study, we investigated the effect of RBC properties on the microcapillary in vitro flow behavior and their characteristic shapes in microfluidic channels. For this, we fractioned RBCs from healthy donors according to their age. Moreover, the membranes of fresh RBCs were chemically rigidified using diamide to study the effect of isolated graded-membrane rigidity. Our results show that a fraction of stable, asymmetric, off-centered slipper-like cells at high velocities decreases with increasing age or diamide concentration. However, while old cells form an enhanced number of stable symmetric croissants at the channel centerline, this shape class is suppressed for purely rigidified cells with diamide. Our study provides further knowledge about the distinct effects of age-related changes of intrinsic cell properties on the single-cell flow behavior of RBCs in confined flows due to inter-cellular age-related cell heterogeneity.

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Section: In Vivo Rbc Imagingmentioning

confidence: 99%

Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow

Nouaman,

Darras,

John

et al. 2023

Cells

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“…In such 2D geometries, similar to our in-plane second generation bifurcations, RBC partitioning is observed in cascades of bifurcations. 19,[45][46][47] This phenomenon is connected to lingering of RBCs at the apex of the bifurcation, which is more pronounced when RBCs are aggregated. 48 Similarly, anomalies in the channels structure can alter RBC partitioning at a downstream bifurcation, which is an effect that depends on hematocrit.…”

Section: Skewness Of Flow Profilementioning

confidence: 99%

Effect of in-plane and out-of-plane bifurcated microfluidic channels on the flow of aggregating red blood cells

Gholivand,

Korculanin,

Dahlhoff

et al. 2024

Lab Chip

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“…However, one of the reviewers encouraged us to include this discussion. In vivo investigations of single RBC properties are rather sparse and mostly limited to animal models [49,94]. Therefore, this subsection is, to some extent, speculative in nature.…”

Section: Physiological Function Of Ion Channels In Red Blood Cellsmentioning

confidence: 99%

The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells

Petkova-Kirova,

Murciano,

Iacono

et al. 2024

IJMS

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The Gárdos channel (KCNN4) and Piezo1 are the best-known ion channels in the red blood cell (RBC) membrane. Nevertheless, the quantitative electrophysiological behavior of RBCs and its heterogeneity are still not completely understood. Here, we use state-of-the-art biochemical methods to probe for the abundance of the channels in RBCs. Furthermore, we utilize automated patch clamp, based on planar chips, to compare the activity of the two channels in reticulocytes and mature RBCs. In addition to this characterization, we performed membrane potential measurements to demonstrate the effect of channel activity and interplay on the RBC properties. Both the Gárdos channel and Piezo1, albeit their average copy number of activatable channels per cell is in the single-digit range, can be detected through transcriptome analysis of reticulocytes. Proteomics analysis of reticulocytes and mature RBCs could only detect Piezo1 but not the Gárdos channel. Furthermore, they can be reliably measured in the whole-cell configuration of the patch clamp method. While for the Gárdos channel, the activity in terms of ion currents is higher in reticulocytes compared to mature RBCs, for Piezo1, the tendency is the opposite. While the interplay between Piezo1 and Gárdos channel cannot be followed using the patch clamp measurements, it could be proved based on membrane potential measurements in populations of intact RBCs. We discuss the Gárdos channel and Piezo1 abundance, interdependencies and interactions in the context of their proposed physiological and pathophysiological functions, which are the passing of small constrictions, e.g., in the spleen, and their active participation in blood clot formation and thrombosis.

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Red blood cell lingering modulates hematocrit distribution in the microcirculation

Cited by 13 publications

References 48 publications

Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow

Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow

Effect of in-plane and out-of-plane bifurcated microfluidic channels on the flow of aggregating red blood cells

The Gárdos Channel and Piezo1 Revisited: Comparison between Reticulocytes and Mature Red Blood Cells

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