Red blood cell membrane-mediated fusion of hydrophobic quantum dots with living cell membranes for cell imaging

Guo, Xi; Zhang, Yongfeng; Liu, Jianbo; Yang, Xiaohai; Huang, Jin; Li, Li; Wan, Lan; Wang, Kemin

doi:10.1039/c6tb01067a

Cited by 24 publications

(21 citation statements)

References 37 publications

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“…If light excitation results in the optical cargo generating fluorescence emission, it can be used for various imaging applications. For example, Guo et al developed constructs made from hydrophobic QDs coated in membrane lipids extracted from RBCs [ 16 ]. The constructs are able to fuse with the cell membrane, allowing the QDs to be embedded in the membrane for imaging applications as well as tracking cell movement.…”

Section: Potential Clinical Applicationsmentioning

confidence: 99%

“…More recently, RBC-based payload delivery systems have been extensively reviewed [ 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ], and new sub-fields, such as systems designed for the delivery of cancer therapeutics [ 13 ] and vascular imaging [ 14 ], are emerging. A particular use of RBC-derived constructs is in relation to loading of optical cargos such as quantum dots (QDs) [ 15 , 16 , 17 ], metallic materials [ 18 , 19 , 20 ], and organic molecules [ 21 , 22 , 23 ], in order to use the constructs for optical imaging and sensing, and phototherapeutic applications. This review specifically covers the development of RBC-derived constructs for light-based theranostics and summarizes some of the current potential clinical applications for these constructs.…”

Section: Introductionmentioning

confidence: 99%

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Phototheranostics Using Erythrocyte-Based Particles

et al. 2021

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There has been a recent increase in the development of delivery systems based on red blood cells (RBCs) for light-mediated imaging and therapeutic applications. These constructs are able to take advantage of the immune evasion properties of the RBC, while the addition of an optical cargo allows the particles to be activated by light for a number of promising applications. Here, we review some of the common fabrication methods to engineer these constructs. We also present some of the current light-based applications with potential for clinical translation, and offer some insight into future directions in this exciting field.

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Section: Potential Clinical Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phototheranostics Using Erythrocyte-Based Particles

et al. 2021

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“…Cell imaging is the basis for many other biological imaging. As far as the research of QDs in cell imaging applications is concerned, it can be summarized as staining (Rajender, Goswami, & Giri, 2019), probe (Zhang et al, 2018) and living cell tracking (Guo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Huang

Tang

2020

J of Applied Toxicology

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Quantum dots (QDs), as one of the emerging nanomaterials, have been widely studied by scientists due to their advantages and potential in bioimaging, especially cell imaging. Cadmium (Cd)‐based QDs, which have the best photoluminescence property, have received widespread attention. However, due to the obvious toxicity problem of these QDs, their cell imaging application is hindered. Recently, the emergence of Cd‐free‐metal and metal‐free QDs with lower toxicity makes people consider that Cd‐based QDs may be substituted by these QDs. However, problems of these QDs in cytotoxicity also cannot be ignored. Some reports claimed that their prepared emerging QDs for cell imaging were low toxicity, but there still exist up‐regulation of oxidative stress, inflammation, and apoptosis from other reports. And, up to now, few reports have separately summarized and discussed the issues of cell imaging and cytotoxicity of different types of QDs. Therefore, in this review, we classify QDs as following three types, Cd‐based, Cd‐free‐metal and metal‐free QDs, focus on the cell, the essential unit of life, discuss cell imaging application and cytotoxicity of QDs, and finally elucidate main mechanisms of cytotoxicity respectively. This review provides reference for the toxicity evaluation of QDs and highlights the potential cytotoxicity of Cd‐free QDs.

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“…For example, very small QDs (<3 nm diameter) were incorporated into the lipid bilayer of fusogenic vesicles that were subsequently delivered into membranes of human embryonic kidney (HEK) 293 cells via vesicle fusion in QDs . In another study, small QDs coated by membranes of red blood cells were fused into human hepatoma cells as cell imaging agents …”

Section: The Bmps' Membrane Insertion and Retention Challengementioning

confidence: 99%

“…Since phospholipid bilayers are typically 4–5 nm thick, the inclusion of hydrophobic NPs within the bilayer is size limited to ≈5 nm in diameter, including the thickness of the organic coat (Figure c). Inclusion of larger hydrophobic NPs is thermodynamically unfavorable due to the energetic penalty associated with protrusion of hydrophobic ligands into the polar solvent . This limitation practically excludes higher order structures and functionalities that could be beneficial in terms of signal or actuation strength.…”

Section: The Bmps' Membrane Insertion and Retention Challengementioning

confidence: 99%

Interfacing the Cell with “Biomimetic Membrane Proteins”

Grupi

Ashur

Degani-Katzav

et al. 2019

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Integral membrane proteins mediate a myriad of cellular processes and are the target of many therapeutic drugs. Enhancement and extension of the functional scope of membrane proteins can be realized by membrane incorporation of engineered nanoparticles designed for specific diagnostic and therapeutic applications. In contrast to hydrophobic insertion of small amphiphilic molecules, delivery and membrane incorporation of particles on the nanometric scale poses a crucial barrier for technological development. In this perspective, the transformative potential of biomimetic membrane proteins (BMPs), current state of the art, and the barriers that need to be overcome in order to advance the field are discussed.

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Red blood cell membrane-mediated fusion of hydrophobic quantum dots with living cell membranes for cell imaging

Abstract: A biomimetic route to fusion of hydrophobic quantum dots (QDs) with living cells for membrane imaging was proposed. Red blood cell membrane lipids acted as both an efficient surfactant to phase-transfer QDs and a fusion reagent to facilitate fusion with cell membranes.

Cited by 24 publications

References 37 publications

Phototheranostics Using Erythrocyte-Based Particles

Phototheranostics Using Erythrocyte-Based Particles

Research advance on cell imaging and cytotoxicity of different types of quantum Dots

Interfacing the Cell with “Biomimetic Membrane Proteins”

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