Red blood cell metabolism and preservation

Hess, John R.; D’Alessandro, Angelo

doi:10.1002/9781119719809.ch14

Cited by 15 publications

(22 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During oxidative stress, 10% of glucose is used by glycolysis and up to 90% is utilized by the pentose phosphate pathway 33 . Based on our calculations, there is a net gain of 24 2,3‐BPG, 94 ATP, and 12 NADPH molecules during non‐stressed conditions, whereas there is a net loss of 12 ATP and a gain of 6 2,3‐BPG and 108 NADPH molecules during oxidative stress.…”

Section: The Dynamics Of Erythrocyte Metabolismmentioning

confidence: 76%

“…Moreover, administration of an oral pyruvate kinase activator in patients with thalassaemia increased haemoglobin and ATP concentration and decreased reactive species concentration 396 . A similar strategy is followed during blood storage, where erythrocytes are treated with various energy and antioxidant molecules for more efficient transfusion 33,397–400 . For instance, rejuvenation of erythrocytes during blood storage (using pyruvate, inosine, phosphate, and adenine) increased the levels of 2,3‐BGP and ATP similar to or even above the levels of fresh cells, probably by enhancing glycolytic flux 401 .…”

Section: The Dynamics Of Erythrocyte Metabolismmentioning

confidence: 99%

See 1 more Smart Citation

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

Self Cite

View full text Add to dashboard Cite

Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map. The focus of this map is on the main biochemical pathways consisting of glycolysis, the pentose phosphate pathway, redox metabolism, oxygen metabolism, purine/nucleoside metabolism, and membrane transport. Other recently emerging pathways are also curated, like the methionine salvage pathway, the glyoxalase system, carnitine metabolism, and the lands cycle, as well as remnants of the carboxylic acid metabolism. An additional goal of this review is to present the dynamics of erythrocyte metabolism, providing key numbers used to perform basic quantitative analyses. By synthesizing experimental and computational data, we conclude that glycolysis, pentose phosphate pathway, and redox metabolism are the foundations of erythrocyte metabolism. Additionally, the erythrocyte can sense oxygen levels and oxidative stress adjusting its mechanics, metabolism, and function. In conclusion, fine‐tuning of erythrocyte metabolism controls one of the most important biological processes, that is, oxygen loading, transport, and delivery.

show abstract

Section: The Dynamics Of Erythrocyte Metabolismmentioning

confidence: 76%

Section: The Dynamics Of Erythrocyte Metabolismmentioning

confidence: 99%

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Surface senescence markers, including Band 3-IgG complexes and phosphatidylserine, tag RBCs for erythrophagocytosis while structural changes such as spheroechinocytosis lead to mechanical removal in the spleen. 23,24 However, a simple loss of circulating RBCs may not be the whole story. A recent study of senescent RBC clearance in a mouse model discovered that erythrophagocytosis downregulates STAT1, a transcription factor for antiviral and antibacterial host response.…”

Section: Proposed Infectious Mechanisms Of Aging Rbcsmentioning

confidence: 99%

“…A second mechanism of possible clinical impact is the accelerated removal of RBCs from circulation. Surface senescence markers, including Band 3‐IgG complexes and phosphatidylserine, tag RBCs for erythrophagocytosis while structural changes such as sphero‐echinocytosis lead to mechanical removal in the spleen 23,24 . However, a simple loss of circulating RBCs may not be the whole story.…”

Section: Clinical Outcomes and Pathophysiologic Mechanismsmentioning

confidence: 99%

Transfusion of red blood cells ≥35 days old: A narrative review of clinical outcomes

Riley,

Stansbury,

Hasan

et al. 2023

Transfusion

Self Cite

View full text Add to dashboard Cite

“…In recent works, the understanding of RBC metabolism has been furthered through the application of the "-omic" sciences, predominately lipidomics and proteomics, in an effort to improve preservation and storage conditions of these cells for transfusion therapy [24]. As a result, RBCs are now known to be metabolically active cellular compartments which express a sophisticated metabolism [25,26]; this makes RBCs an intriguing prospect for metabolomic studies of MTX [26,27]. Therefore, the biochemical composition of RBCs and related biochemical pathways represents a cellular phenotype that may be informative about disease pathology and treatment response [22,25,28].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling of Red Blood Cells to Identify Molecular Markers of Methotrexate Response in the Collagen Induced Arthritis Mouse Model

Salamoun

Polireddy

Cho

et al. 2022

Future Pharmacology

View full text Add to dashboard Cite

Although methotrexate (MTX) is the first line disease-modifying therapy used in the treatment of autoimmune arthritis, it is limited by its unpredictable and variable response profile and lack of therapeutic biomarkers to predict or monitor therapeutic response. The purpose of this work is to evaluate the utility of red blood cell (RBC) metabolite profiles to screen for molecular biomarkers associated with MTX response. Methods: Utilizing the collagen-induced arthritis mouse model, DBA/1J mice were treated with subcutaneous MTX (20 mg/kg/week) and RBC samples were collected and analyzed by semi-targeted global metabolomic profiling and analyzed by univariate analysis. Results: MTX treatment normalized the following RBC metabolite levels that were found to be altered by disease induction: N-methylisoleucine, nudifloramide, phenylacetylglycine, 1-methyl-L-histidine, PC 42:1, PE 36:4e, PC 42:3, PE 36:4e (16:0e/20:4), and SM d34:0. Changes in the RBC metabolome weakly but significantly correlated with changes in the plasma metabolome following MTX treatment (ρ = 0.24, p = 1.1 × 10−13). The RBC metabolome resulted in the detection of nine significant discriminatory biomarkers, whereas the plasma metabolome resulted in two. Overall, the RBC metabolome yielded more highly sensitive and specific biomarkers of MTX response compared to the plasma metabolome. N-methylisoleucine was found to be highly discriminatory in both plasma and RBCs. Conclusions: Our results suggest that RBCs represent a promising biological matrix for metabolomics and future studies should consider the RBC metabolome in their biomarker discovery strategy.

show abstract

Red blood cell metabolism and preservation

Cited by 15 publications

References 5 publications

Erythrocyte metabolism

Erythrocyte metabolism

Transfusion of red blood cells ≥35 days old: A narrative review of clinical outcomes

Metabolomic Profiling of Red Blood Cells to Identify Molecular Markers of Methotrexate Response in the Collagen Induced Arthritis Mouse Model

Contact Info

Product

Resources

About