Red blood cell morphology

Ford, Jason C.

doi:10.1111/ijlh.12082

Cited by 189 publications

(138 citation statements)

References 12 publications

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“…This frequency sweep technique was further extended to negatively charged biconcave red blood cells (RBCs), which are an important cellular system for medical disease diagnostics. [36][37][38] …”

Section: Introductionmentioning

confidence: 99%

Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells

2013

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Alternating current (AC) dielectrophoresis (DEP) experiments for biological particles in microdevices are typically done at a fixed frequency. Reconstructing the DEP response curve from static frequency experiments is laborious, but essential to ascertain differences in dielectric properties of biological particles. Our lab explored the concept of sweeping the frequency as a function of time to rapidly determine the DEP response curve from fewer experiments. For the purpose of determining an ideal sweep rate, homogeneous 6.08 lm polystyrene (PS) beads were used as a model system. Translatability of the sweep rate approach to $7 lm red blood cells (RBC) was then verified. An Au/Ti quadrapole electrode microfluidic device was used to separately subject particles and cells to 10Vpp AC electric fields at frequencies ranging from 0.010 to 2.0 MHz over sweep rates from 0.00080 to 0.17 MHz/s. PS beads exhibited negative DEP assembly over the frequencies explored due to Maxwell-Wagner interfacial polarizations. Results demonstrate that frequency sweep rates must be slower than particle polarization timescales to achieve reliable incremental polarizations; sweep rates near 0.00080 MHz/s yielded DEP behaviors very consistent with static frequency DEP responses for both PS beads and RBCs. V C 2013 AIP Publishing LLC.

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Section: Introductionmentioning

confidence: 99%

Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells

2013

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“…Heinz bodies are denatured haemoglobin (seen in oxidant injury, G6PD deficiency). Haemoglobin H inclusions are seen in alpha-thalassemias giving rise to the characteristic 'golf ball' appearance of the erythrocytes [13,14,36].…”

Section: Other Red Cell Anomaliesmentioning

confidence: 99%

“…As such, there is a need for practicing physicians to be conversant with current approaches to anaemia diagnosis. Despite advances in haematology automations, flow cytometry/immunophenotyping and molecular biology techniques, red cell morphology remains a vital aspect of anaemia work up [13]. In the present communication an attempt has been made to review the morphologic evaluation of anaemias and their clinical interpretations.…”

Section: Introductionmentioning

confidence: 98%

Morphologic Evaluation of Anemia I

Ademola¹,

Abiola²

2016

Biol Med (Aligarh)

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Anaemia is a feature of many tropical diseases. Anaemia diagnosis therefore remains a crucial intervention among physicians in developing countries. A barrage of laboratory test (anaemic work-up) is usually deployed in differentiating its underlying cause. However, central to anaemia evaluation is the morphology of the red cells and other cell lines. Conventionally, initial laboratory tests include full blood count, reticulocyte count and peripheral blood film (PBF). PBF is often a clinical request, performed by skilled technologist and reported by haematologist/ haematomorphologist. Findings from PBF are reviewed and reported in the light of patient's clinical history and examination findings. This article therefore aims to promote PBF evaluation in anaemic patients, facilitate laboratory communication of morphologic findings among clinicians, particularly in developing nations where advanced investigations such as flow cytometry and molecular diagnosis may not be readily available, invariably improving patient care and treatment outcomes.

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“…Abnormalities of RBC shape and other RBC features can provide key information in establishing a differential diagnosis. 2 The laboratory evaluation of anemia begins with a complete blood count and reticulocyte count. The anemia is then categorized as microcytic, macrocytic or normocytic, with or without reticulocytosis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anaemia

Ingale¹,

Ullewar

Ingale

et al. 2017

Int J Res Med Sci

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Background: Often, the first test used to diagnose anemia is a complete blood count (CBC). It determines the number, size, volume, and hemoglobin content of red blood cells. Peripheral smear is done for typing of anaemia. Such evaluation is necessary for proper treatment.Methods: A retrospective study was done in 300 anaemic patients at Shakuntala pathology laboratory, Nagpur. Patients were randomly selected including males and females. CBC and peripheral smear were analyzed.Results: Out of total 300 patients evaluated, the prevalence of anaemia was quite significant in females 225 (75%) than males 75 (25%). 66 females (22%) had mild anemia while 129 females (43%) had moderate anemia and 30 females (10%) had severe anaemia 36 males (12%) had mild anaemia, 30 males (10%) had moderate anaemia while 9 males (3%) had severe anaemia. In morphology of red blood cells, normocytic normochromic anaemia was seen in 132 (44%) females and in 45 (15%) of males. Microcytic hypochromic anaemia was seen in 90 (30%) females and 27 (9%) males. Macrocytic anaemia was seen in 3 (1%) females and 3 (1%) males.Conclusions: Prevalence of anaemia is quite high in females than males. Also, the severity of anaemia is more in females than males. So, heath programmes should be directed more towards females since adolescent age.

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Red blood cell morphology

Cited by 189 publications

References 12 publications

Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells

Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells

Morphologic Evaluation of Anemia I

Evaluation of anaemia

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