Red blood cell senescence and neocytolysis in humans after high altitude acclimatization

Risso, Angela; Turello, Marina; Biffoni, Franco; Antonutto, Guglielmo

doi:10.1016/j.bcmd.2006.10.161

Cited by 73 publications

(88 citation statements)

References 34 publications

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“…After centrifugation, two layers of RBCs are present; those having a density of less than the 1.105 g mL Percoll solution will appear at the top of the tube and represent those RBCs having the lowest density; these RBCs are considered the "younger" of the RBCs in the original RBC sample. 35,36 Those below the higher density Percoll solution are the more dense, or aged, RBCs. These separated RBC fractions were removed by pipette and diluted to a hematocrit of 7% using the PSS described above.…”

Section: Separation Of Rbcs Into Age-based Fractionsmentioning

confidence: 99%

Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes

Subasinghe¹,

Spence²

2008

Analytica Chimica Acta

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Glucose-6-phosphate dehydrogenase (G6PD) is a determinant in the antioxidant status of the red blood cell (RBC) and is also used as an indicator of cell age. However, it is unknown if the relationship between antioxidant status, cell age, and RBC-derived adenosine triphosphate (ATP) occurs immediately or over a period of time. Therefore, the development of a simultaneous determination of G6PD activity (via the determination of nicotinamide adenine dinucleotide phosphate (NADPH)) in RBCs and the determination of deformation-induced RBC-derived ATP is described. The NADPH and ATP were determined while undergoing a chemically-induced aging process via inhibition of G6PD with dehydroepiandroesterone (DHEA). Upon incubation with DHEA for 30 minutes, NADPH levels measured in a flow stream decreased to 7.96 ± 1.10 μM from an original value of 13.20 ± 1.80 μM in a 0.02% solution of RBCs. In order to demonstrate a direct relationship between G6PD activity and deformation-induced ATP release from RBCs, a simultaneous microflow determination of G6PD activity and ATP release was performed. Upon inhibition with DHEA, NADPH levels decreased to 8.62 ± 0.29 μM from its original value of 12.73 ± 0.50 μM while ATP release decreased from 0.21 ± 0.07 μM to 0.06 ± 0.02 μM. These values were validated by an examination of NADPH levels in, and ATP release from, RBC fractions containing younger and older cells (separated by cell density centrifugation). This determination provides evidence that antioxidant status in the RBC and its ability to release ATP, a known stimulus of nitric oxide production, are closely related.

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Section: Separation Of Rbcs Into Age-based Fractionsmentioning

confidence: 99%

Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes

Subasinghe¹,

Spence²

2008

Analytica Chimica Acta

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“…The indices restored to normal one to two months after the climbers returned to the plain. Risso and others [14] assumed that when mountain climbers returned to the plain from the plateau, abnormal environmental stimulants disappeared and brought no harm to the human body. However, most scholars reported that when high altitude natives (43 -50 years old) traveled to sea level, their stroke index (SI) increased, their heart rate decreased, and the cardiac index (CI) remained unchanged [15] [16].…”

Section: Discussionmentioning

confidence: 99%

Regular Observation of De-Acclimatization and Randomized Controlled Research of Diagnostic Criteria of High Altitude De-Acclimatization Syndrome among Different Plateau Migrants Crowd after Their Return to the Plain

Zhou¹,

Yang²,

ZhenCai³

et al. 2014

ODEM

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“…Post-hypoxia RBC counts, haematocrit, and haemoglobin concentration were higher than pre-hypoxia levels while plasma erythropoietin (EPO) levels were decreased [2]. Furthermore, when centrifuged on discontinuous Percoll density gradient, most of the RBCs (97.90 ± 2.00%) sedimented as high-density/old cells.…”

Section: Resultsmentioning

confidence: 99%

“…Related to these changes is the ‘senescent-like’ phenotype we observed in RBCs isolated from blood samples from mountain climbers 24 h after their return to a normoxic environment [2]. In fact, when we separated age-related RBC subsets by density, we found that young RBCs had disappeared and middle-aged RBCs had sharply decreased due to neocytolysis, i.e.…”

Section: Introductionmentioning

confidence: 90%

“…The majority of the latter (97.90 ± 2%) sedimented as high-density RBCs when centrifuged on Percoll density gradient and showed decreased expression of the membrane proteins CD55 and CD59, which are partially lost during the in vitro and in vivo RBC ageing process [6,7]. The senescent-like phenotype was further confirmed by exposure of phosphatidylserine on the membrane outer leaflet of a small percentage of RBCs and the lower expression of CD47 [2]. In fact, phosphatydilserine and CD47 seem to be involved in triggering [8,9] or inhibiting [10,11,12], respectively, RBC phagocytosis by macrophages.…”

Section: Introductionmentioning

confidence: 94%

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Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia

et al. 2009

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In a comparative study on erythrocytes (RBCs) drawn from mountaineers before and after a high-altitude stay, we observed that upon returning to sea level, their RBCs displayed a senescent-like phenotype as indicated by their density and the partial loss of membrane proteins which are shed by ageing RBCs. The aim of this study was to investigate possible changes in the membrane skeleton of these RBCs and to compare them with pathological RBCs. We analysed the proteins of RBC ghosts obtained from our subjects before and after returning to sea level by two-dimensional electrophoresis and mass spectrometry. We observed lower expression and fragmentation of β-actin after exposure to hypoxia. This suggested an alteration in membrane skeleton structure, which was confirmed by β-actin release in cell lysates during ghost preparation. We observed a similar actin fragmentation and release in RBC lysates from β-thalassaemic patients. In conclusion, these results indicate that after exposure to hypoxia, RBCs display a modification of their actin and cytoskeleton instability.

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Red blood cell senescence and neocytolysis in humans after high altitude acclimatization

Cited by 73 publications

References 34 publications

Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes

Simultaneous determination of cell aging and ATP release from erythrocytes and its implications in type 2 diabetes

Regular Observation of De-Acclimatization and Randomized Controlled Research of Diagnostic Criteria of High Altitude De-Acclimatization Syndrome among Different Plateau Migrants Crowd after Their Return to the Plain

Fragmentation of Human Erythrocyte Actin following Exposure to Hypoxia

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