Red blood cell shape transitions and dynamics in time-dependent capillary flows

Recktenwald, Steffen M.; Graessel, Katharina; Maurer, Felix; John, Thomas; Gekle, Stephan; Wagner, Christian

doi:10.1016/j.bpj.2021.12.009

Cited by 30 publications

(37 citation statements)

References 90 publications

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“…Figure 1D shows the phase diagram for a healthy control sample. Here, croissant-like RBC shapes dominate at lower velocities, while slipper-shaped RBCs predominantly emerge above velocities of 6 mm/s, in accordance with previous experimental and numerical studies on similar channels ( Guckenberger et al, 2018 ; Recktenwald et al, 2022 ).…”

Section: Methodssupporting

confidence: 91%

“…At low velocities, RBCs preferentially form axisymmetric croissants that flow in the channel center at |y/W| = 0, while slipper-shaped RBCs dominate at higher velocities, which exhibit an off-centered equilibrium position at |y/W| > 0. The distribution of the RBC lateral y -position for a given velocity is a characteristic indicator of the single-cell flow in such confined microchannels ( Guckenberger et al, 2018 ; Kihm et al, 2018 ; Recktenwald et al, 2022 ) and was used as a reference point for pathological changes in RBC flow behavior at specific cell velocities. Data processing was roughly half as fast as the acquisition and started almost simultaneously (but scales with CPU power).…”

Section: Methodsmentioning

confidence: 99%

“…Our training data set consisted of seven different classes. Besides the characteristic croissant and slipper shapes that dominate healthy RBC flow in microchannels ( Figure 1B ) ( Guckenberger et al, 2018 ; Recktenwald et al, 2022 ), complimentary pathological classes that exhibit pathophysiologic RBC shapes were identified. A representative overview of all RBC shape classes is provided in Supplementary Figure S1 of the Supplementary Material.…”

Section: Methodsmentioning

confidence: 99%

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Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications

et al. 2022

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In many medical disciplines, red blood cells are discovered to be biomarkers since they “experience” various conditions in basically all organs of the body. Classical examples are diabetes and hypercholesterolemia. However, recently the red blood cell distribution width (RDW), is often referred to, as an unspecific parameter/marker (e.g., for cardiac events or in oncological studies). The measurement of RDW requires venous blood samples to perform the complete blood cell count (CBC). Here, we introduce Erysense, a lab-on-a-chip-based point-of-care device, to evaluate red blood cell flow properties. The capillary chip technology in combination with algorithms based on artificial neural networks allows the detection of very subtle changes in the red blood cell morphology. This flow-based method closely resembles in vivo conditions and blood sample volumes in the sub-microliter range are sufficient. We provide clinical examples for potential applications of Erysense as a diagnostic tool [here: neuroacanthocytosis syndromes (NAS)] and as cellular quality control for red blood cells [here: hemodiafiltration (HDF) and erythrocyte concentrate (EC) storage]. Due to the wide range of the applicable flow velocities (0.1–10 mm/s) different mechanical properties of the red blood cells can be addressed with Erysense providing the opportunity for differential diagnosis/judgments. Due to these versatile properties, we anticipate the value of Erysense for further diagnostic, prognostic, and theragnostic applications including but not limited to diabetes, iron deficiency, COVID-19, rheumatism, various red blood cell disorders and anemia, as well as inflammation-based diseases including sepsis.

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Section: Methodssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications

et al. 2022

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“…A microfluidic channel with a rectangular cross-section of 8 μm height (H) and 11 μm width (W) (Figure 1B) is used to pump RBC suspensions as described (17). RBC flow x-y-plane is recorded with a frame rate of up to 400 fps using a pressure drop range between 100 mbar and 1000 mbar.…”

Section: Methodsmentioning

confidence: 99%

“…At such high velocities, RBC deformability turned out to be heterogeneous, with most of the RBCs being strongly elongated in the flow direction but some exhibiting circular, less deformable shapes compared with healthy controls. In human capillaries, which have diameters similar to RBC size (about 10 μm), confined flows confer to RBCs characteristic shapes that depend on the cell velocity and the surrounding medium (16, 17). We previously used a microfluidic setup with channel dimensions similar to the RBC size and analyzed RBC shapes at high detail by means of artificial neural networks (18) in a velocity range that resembles microvascular in vivo flow.…”

Section: Introductionmentioning

confidence: 99%

Cross-talk between red blood cells and plasma influences blood flow and omics phenotypes in severe COVID-19

Recktenwald

Simionato

Lopes

et al. 2022

Preprint

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Coronavirus disease 2019 (COVID-19) is caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and can affect multiple organs, among which is the circulatory system. Inflammation and mortality risk markers were previously detected in COVID-19 plasma and red blood cells (RBCs) metabolic and proteomic profiles. Additionally, biophysical properties, such as deformability, were found to be changed during the infection. Based on such data, we aim to better characterize RBC functions in COVID-19. We evaluate the flow properties of RBCs in severe COVID-19 patients admitted to the intensive care unit by using in vitro microfluidic techniques and automated methods, including artificial neural networks, for an unbiased RBC analysis. We find strong flow and RBC shape impairment in COVID-19 samples and demonstrate that such changes are reversible upon suspension of COVID-19 RBCs in healthy plasma. Vice versa, healthy RBCs immediately resemble COVID-19 RBCs when suspended in COVID-19 plasma. Proteomics and metabolomics analyses allow us to detect the effect of plasma exchanges on both plasma and RBCs and demonstrate a new role of RBCs in maintaining plasma equilibria at the expense of their flow properties. Our findings provide a framework for further investigations of clinical relevance for therapies against COVID-19 and possibly other infectious diseases.

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“Live” Nanomaterials Process Biomimetic Recognition and Assembly In Vivo

Zhang,

Li,

Huang

et al. 2023

Small Science

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The biomimetic strategy has been widely used in multiple fields, and provides huge convenience to human life. For biomedical application in vivo, the importance of biomimetic materials lies on mimicking the natural process/behavior as well as mimicking the structure/ingredient. Herein, recent advances of materials processing biomimetic recognition and assembly behavior for in vivo applications, including the natural source materials and artificial materials, are summarized. The biological processes are divided as recognition and assembly as well as recognition and self‐amplifying assembly. Finally, to conclude, the application of recognition and assembly process‐biomimetic materials in vivo in the future is discussed.

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Red blood cell shape transitions and dynamics in time-dependent capillary flows

Cited by 30 publications

References 90 publications

Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications

Erysense, a Lab-on-a-Chip-Based Point-of-Care Device to Evaluate Red Blood Cell Flow Properties With Multiple Clinical Applications

Cross-talk between red blood cells and plasma influences blood flow and omics phenotypes in severe COVID-19

“Live” Nanomaterials Process Biomimetic Recognition and Assembly In Vivo

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