Red blood cell storage affects the stability of cytosolic native protein complexes

Pallotta, Valeria; Rinalducci, Sara; Zolla, Lello

doi:10.1111/trf.13079

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…These changes may reflect alterations in enzyme and/or membrane transport activity. Glycolytic enzymes are organized in multi-protein complexes that bind to band 3 and/or various other membrane proteins, and changes in band 3 conformation regulate their activity [44,45,[61][62][63]. Metabolomic data of misshapen RBCs support the hypothesis that alterations in membrane protein conformation affect various metabolic pathways [2,[64][65][66].…”

Section: Metabolome Changes As Clues To Vesiculation Mechanismsmentioning

confidence: 99%

Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

et al. 2020

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Microvesicle generation is an integral part of the aging process of red blood cells in vivo and in vitro. Extensive vesiculation impairs function and survival of red blood cells after transfusion, and microvesicles contribute to transfusion reactions. The triggers and mechanisms of microvesicle generation are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, and metabolomic analyses to obtain an integrated understanding of the mechanisms underlying microvesicle generation during the storage of red blood cell concentrates. Our data indicate that changes in membrane organization, triggered by altered protein conformation, constitute the main mechanism of vesiculation, and precede changes in lipid organization. The resulting selective accumulation of membrane components in microvesicles is accompanied by the recruitment of plasma proteins involved in inflammation and coagulation. Our data may serve as a basis for further dissection of the fundamental mechanisms of red blood cell aging and vesiculation, for identifying the cause-effect relationship between blood bank storage and transfusion complications, and for assessing the role of microvesicles in pathologies affecting red blood cells.

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Section: Metabolome Changes As Clues To Vesiculation Mechanismsmentioning

confidence: 99%

Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

et al. 2020

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“…RBCs undergo time‐dependent deterioration in several aspects of their physiology during storage in standard blood bank conditions, collectively named as “RBC storage lesion.” In this context, functionally important disturbances in energy and redox metabolism, rheology, and finally, in RBC aging and removal signaling are apparent after examination of RBC concentrates in vitro by both targeted and integrated, omics‐based approaches . Indeed, time‐dependent changes in the expression/accumulation and posttranslational modifications of hemoglobin (Hb), supernatant and membrane proteins and lipids, including fragmentation, oxidation/carbonylation, oligomerization, destabilization, and phosphorylation have been reported as candidate biomarkers of storage quality and posttransfusion effects in RBC units . Omics studies have expanded knowledge on the metabolic, biomechanical, and oxidative effects of storage on RBCs and their molecular basis as a function of storage duration, strategy, and probable clinical relevance .…”

Section: Red Blood Cells Are Affected By Storage—are the Recipients Amentioning

confidence: 99%

“…biomarkers of storage quality and posttransfusion effects in RBC units [2][3][4][5][6]. Omics studies have expanded knowledge on the metabolic, biomechanical, and oxidative effects of storage on RBCs and their molecular basis as a function of storage duration, strategy, and probable clinical relevance [7][8][9][10].…”

mentioning

confidence: 99%

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Tzounakas¹,

Kriebardis

Papassideri³

et al. 2016

Proteomics Clinical Apps

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Although the molecular pathways leading to the progressive deterioration of stored red blood cells (RBC storage lesion) and the clinical relevance of storage-induced changes remain uncertain, substantial donor-specific variability in RBC performance during storage, and posttransfusion has been established ("donor-variation effect"). In-bag hemolysis and numerous properties of the RBC units that may affect transfusion efficacy have proved to be strongly donor-specific. Donor-variation effect may lead to the production of highly unequal blood labile products even when similar storage strategy and duration are applied. Genetic, undiagnosed/subclinical medical conditions and lifestyle factors that affect RBC characteristics at baseline, including RBC lifespan, energy metabolism, and sensitivity to oxidative stress, are all likely to influence the storage capacity of individual donors' cells, although not evident by the donor's health or hematological status at blood donation. Consequently, baseline characteristics of the donors, such as membrane peroxiredoxin-2 and serum uric acid concentration, have been proposed as candidate biomarkers of storage quality. This review article focuses on specific factors that might contribute to the donor-variation effect and emphasizes the emerging need for using omics-based technologies in association with in vitro and in vivo transfusion models and clinical trials to discover biomarkers of storage quality and posttransfusion recovery in donor blood.

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“…The low temperature also inhibits metabolic enzyme activities, which results in a progressive reduction in ATP [36]. D'Alessandro et al [37] showed that the oxidative phase in the pentose phosphate pathway was activated with prolonged cold storage, at least during the first two weeks of storage; this indicated a high demand on antioxidant activity, such as reduced glutathione (GSH) [34,38]. NADPH is necessary to reduce oxidized glutathione (GSSG) and recycle GSH to counteract potential damage from accumulating reactive oxygen species (ROS).…”

Section: Rbc Storage Lesionsmentioning

confidence: 99%

Red blood cell microvesicles: a storage lesion or a possible salvage mechanism

Delobel

Barelli

Canellini

et al. 2016

ISBT Science Series

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Erythrocyte concentrates are the major labile blood products used for transfusion worldwide. Erythrocyte concentrates are stored at 4°C for up to 42-49 days in additive solutions. This storage induces cellular lesions, which include alterations in red blood cell (RBC) metabolism, protein content and rheological properties. The impact of these storage lesions is intensely debated, because transfusionrelated clinical adverse outcomes have been associated with the age of erythrocyte concentrates. Although altered red blood cell metabolism and early rheological changes are reversible with one transfused, oxidized proteins cannot be repaired. Thus, some irreversible damage might affect the quality of the blood product and the efficiency of the transfusion. In vivo, red blood cells are constantly exposed to oxygen fluxes but are well equipped to deal with oxidative challenges. Moreover, erythrocytes can eliminate oxidized proteins by capturing them in microvesicles, which are subsequently shed into the medium. This review discusses some biochemical aspects of RBC storage and microvesiculation.

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Red blood cell storage affects the stability of cytosolic native protein complexes

Cited by 20 publications

References 24 publications

Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences

Donor‐variation effect on red blood cell storage lesion: A close relationship emerges

Red blood cell microvesicles: a storage lesion or a possible salvage mechanism

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