Red blood cells release microparticles containing human argonaute 2 and miRNAs to target genes of
            <i>Plasmodium falciparum</i>

Wang, Zhensheng; Xi, Jing; Hao, Xiao; Deng, Weiwei; Liu, Juan; Wei, Chunyan; Gao, Yuhui; Zhang, Lian-Hui; Wang, Heng

doi:10.1038/emi.2017.63

Cited by 62 publications

(77 citation statements)

References 53 publications

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“…MiRNAs, human Argonaute protein and RISC complex could be detected in the parasites [40] as well as in EVs released by infected erythrocytes [44,45]. EVs carried Argonaut-miRNAs complex affecting recipient cells that have been demonstrated to alter vascular function [44] or parasite's var gene expression [46]. These studies strengthen the roles of EVs-derived miRNAs in malaria pathogenesis.…”

Section: Introductionmentioning

confidence: 53%

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Ketprasit

Cheng

Deutsch

et al. 2020

Preprint

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Background Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered as promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected samples. In our study, we investigated EV specific miRNAs isolated from the plasma of patients from Thailand infected with Plasmodium falciparum and Plasmodium vivax . We postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes. Methods EVs were purified from the plasma of Thai P.vivax -infected patients (PV, n=19), P.falciparum -infected patients (PF, n=18) and uninfected individuals (n=20). EV-derived miRNAs were then prepared and abundance of miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these samples. Quantitative Polymerase Chain Reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. We then predicted the targets of up-regulated miRNAs and relevant pathways by bioinformatics. Receiver Operating Characteristic with Area Under the Curve (AUC) was then calculated to assess their diagnostic potential. Results The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in PV patients compared to uninfected individuals, but hsa-let-7a-5p was up-regulated in both PV and PF. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the Adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarker as determined by AUC; however, the sensitivity and specificity require further investigation. Conclusion The up-regulated hsa-miR-150-5p and hsa-miR-15b-5p in PV, hsa-let-7a-5p in both PV and PF may play a role in host-parasite interactions. The findings in this study will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EV miRNAs to malaria detection and biology.

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Section: Introductionmentioning

confidence: 53%

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Ketprasit

Cheng

Deutsch

et al. 2020

Preprint

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“…Chamnanchanunt et al found that hsa-miR-451a was down-regulated in P. vivax-infected patient plasma [44]. Other studies demonstrated that EVs cargo hsa-miR-451a could be internalized to target the parasites and diminish the parasite burden [38,43]. Furthermore, in an in vitro study, red blood cell derived EVs containing hsa-miR-451a and human argonaute 2 (Ago2) were shown to be internalized by endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…By studying the EV compartment as a whole the chances of nding differences in miRNA expression between the groups would be increased. Five miRNA were selected based on previous in vitro, animal model, or clinical studies that suggested these miRNAs had a potential involvement in malaria, namely: hsa-miR-451a, hsa-miR-150-5p, hsa-miR-15b-5p, hsa-let-7a, and hsa-miR-16-5p [37][38][39][41][42][43][44]48]. This study provides novel insight about circulating EVs-derived miRNAs in human malaria.…”

mentioning

confidence: 99%

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Ketprasit

Cheng

Deutsch

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Extracellular vesicles (EVs) have been broadly studied in malaria for nearly a decade. These vesicles carry various functional biomolecules including RNA families such as microRNAs (miRNA). These EVs-derived microRNAs have numerous roles in host-parasite interactions and are considered promising biomarkers for disease severity. However, this field lacks clinical studies of malaria-infected samples. In this study, EV specific miRNAs were isolated from the plasma of patients from Thailand infected with Plasmodium vivax and Plasmodium falciparum. In addition, it is postulated that these miRNAs were differentially expressed in these groups of patients and had a role in disease onset through the regulation of specific target genes.Methods EVs were purified from the plasma of Thai P. vivax-infected patients (n=19), P. falciparum-infected patients (n=18) and uninfected individuals (n=20). EV-derived miRNAs were then prepared and abundance of hsa-miR-15b-5p, hsa-miR-16-5p, hsa-let-7a-5p and hsa-miR-150-5p was assessed in these samples. Quantitative polymerase chain reaction was performed, and relative expression of each miRNA was calculated using hsa-miR-451a as endogenous control. Then, the targets of up-regulated miRNAs and relevant pathways were predicted by using bioinformatics. Receiver Operating Characteristic with Area Under the Curve (AUC) was then calculated to assess their diagnostic potential.Results The relative expression of hsa-miR-150-5p and hsa-miR-15b-5p was higher in P. vivax-infected patients compared to uninfected individuals, but hsa-let-7a-5p was up-regulated in both P. vivax-infected patients and P. falciparum-infected patients. Bioinformatic analysis revealed that these miRNAs might regulate genes involved in the malaria pathway including the adherens junction and the transforming growth factor-β pathways. All up-regulated miRNAs could potentially be used as disease biomarkers as determined by AUC; however, the sensitivity and specificity require further investigation.Conclusion An upregulation of hsa-miR-150-5p and hsa-miR-15b-5p was observed in P. vivax-infected patients while hsa-let-7a-5p was up-regulated in both P. vivax-infected and P. falciparum-infected patients. These findings will require further validation in larger cohort groups of malaria patients to fully understand the contribution of these EVs miRNAs to malaria detection and biology.

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“…Subsequent to infection with malaria parasites, iRBCs secrete significantly more extracellular vesicles than uninfected cells (Mantel et al, 2016; Regev-Rudzki et al, 2013). Extracellular vesicles derived from iRBCs contain many factors, including antigens, virulence factors, mammalian-and parasite-derived nucleic acids, and the RNAi machinery (Babatunde et al, 2018;Mantel et al, 2016;Sampaio et al, 2018;Wang et al, 2017).…”

Section: Extracellular Vesicles and Arthropod-borne Microbial Transmimentioning

confidence: 99%

Message in a vesicle – trans-kingdom intercommunication at the vector–host interface

Chávez

O’Neal

Santambrogio

et al. 2019

Journal of Cell Science

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Vector-borne diseases cause over 700,000 deaths annually and represent 17% of all infectious illnesses worldwide. This public health menace highlights the importance of understanding how arthropod vectors, microbes and their mammalian hosts interact. Currently, an emphasis of the scientific enterprise is at the vector-host interface where human pathogens are acquired and transmitted. At this spatial junction, arthropod effector molecules are secreted, enabling microbial pathogenesis and disease. Extracellular vesicles manipulate signaling networks by carrying proteins, lipids, carbohydrates and regulatory nucleic acids. Therefore, they are well positioned to aid in cell-to-cell communication and mediate molecular interactions. This Review briefly discusses exosome and microvesicle biogenesis, their cargo, and the role that nanovesicles play during pathogen spread, host colonization and disease pathogenesis. We then focus on the role of extracellular vesicles in dictating microbial pathogenesis and host immunity during transmission of vector-borne pathogens.

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Red blood cells release microparticles containing human argonaute 2 and miRNAs to target genes of Plasmodium falciparum

Cited by 62 publications

References 53 publications

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

The characterization of extracellular vesicles-derived microRNAs in Thai malaria patients

Message in a vesicle – trans-kingdom intercommunication at the vector–host interface

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