Red blood cells under varying extracellular tonicity conditions: an optical tweezers combined with micro-Raman study

Lukose, Jijo; Shastry, Shamee; Mithun, N; Mohan, Ganesh; Ahmed, Akheel; Chidangil, Santhosh

doi:10.1088/2057-1976/ab6e1a

Cited by 10 publications

(7 citation statements)

References 59 publications

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“…Raman peaks that displayed enhanced intensity in this spectral region were wavenumbers at 1,212, 1,246, and 1,264 cm –1 . Alteration of 1,246 cm –1 is indicative of heme aggregation ( 41 ). This was accompanied by a fall in intensity at the peak of 1,222 cm –1 suggesting changes in the methine C-H deformation ( 35 , 36 ).…”

Section: Resultsmentioning

confidence: 99%

“…A new peak surfaced at 663 cm –1 that shouldered the 675 cm –1 pyrrole-deformation peak across the disease populations as well. Both these events are indicative of heme-aggregation in consequence of protein denaturation ( 40 , 41 ). The porphyrin breathing mode peak at 752 cm –1 , signifying Hb integrity ( 45 ), exhibited a significantly reduced intensity in the disease groups.…”

Section: Resultsmentioning

confidence: 99%

“…This implied Hb breakdown ( 38 , 46 ) and disruption of the RBC membrane ( 43 ). Altered heights of peaks 1,122 and 1,080 cm –1 in the disease groups that emanate from the RBC membrane signified distorted membrane deformability ( 41 ), whereas altered intensities at 1,165 and 1,175 cm –1 characterize deformation in the pyrrole ring ( 47 ).…”

Section: Resultsmentioning

confidence: 99%

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Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections

Jacob

Lukose²,

Bankapur³

et al. 2022

Front. Med.

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Malaria, dengue and leptospirosis are three tropical infectious diseases that present with severe hematological derangement causing significant morbidity and mortality, especially during the seasonal monsoons. During the course of these infectious diseases, circulating red blood cells are imperiled to the direct ill-effects of the infectious pathogen in the body as well as to the pro-inflammatory cytokines generated as a consequence of the infection. RBCs when exposed to such inflammatory and/or pathogenic milieu are susceptible to injuries such as RBC programmed eryptosis or RBC programmed necrosis. This research aimed to explore the Raman spectra of live red cells that were extracted from patients infected with malaria, dengue, and leptospirosis. Red cells were optically trapped and micro-Raman probed using a 785 nm Diode laser. RBCs from samples of all three diseases displayed Raman signatures that were significantly altered from the normal/healthy. Distinct spectral markers that were common across all the four groups were obtained from various standardized multivariate analytical methods. Following comprehensive examination of multiple studies, we propose these spectral wavenumbers as “Raman markers of RBC injury.” Findings in our study display that anemia-triggering infections can inflict variations in the healthy status of red cells, easily identifiable by selectively analyzing specific Raman markers. Additionally, this study also highlights relevant statistical tools that can be utilized to study Raman spectral data from biological samples which could help identify the very significant Raman peaks from the spectral band. This approach of RBC analysis can foster a better understanding of red cell behavior and their alterations exhibited in health and disease.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections

Jacob

Lukose²,

Bankapur³

et al. 2022

Front. Med.

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“…The weak signals in Raman scattering can be enhanced by placing the sample in contact with a metallic nanoparticle surface / roughened metallic film surface in order to satisfy plasmonic condition, which is termed as Surface Enhanced Raman Spectroscopy. The Raman Tweezers technology combines optical tweezers with Raman spectroscopy, where the micron sized particle in aqueous solution will be trapped using a laser beam and will be probed/characterized using Raman spectroscopy [9][10][11]. The FTIR technique is used to capture the infrared spectrum of absorption of substances that are solid, liquid, or gaseous.…”

Section: Introductionmentioning

confidence: 99%

Identification of Microplastics Using a Custom Built Micro-Raman Spectrometer

Unnimaya

Mithun²,

Lukose³

et al. 2023

J. Phys.: Conf. Ser.

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Microplastics are plastic particles less than 5 mm in size but larger than 0.1 µm. Widespread microplastic pollution in aquatic and terrestrial environment is raising serious concern globally due to its detrimental impacts on living beings. This urge for the necessity of a sensitive analytical tool, which can facilitate reliable identification of microplastics. Micro-Raman spectroscopy enables molecular level structural details of samples and thus highly preferable for the identification of microplastics in water. Besides being a non-destructive technique enabling fast analysis, this technique requires minimal/no sample preparation. The present work demonstrates the performance of a custom built Micro-Raman spectrometer for the discrimination of various microplastics in water based samples. Micro- Raman analysis have been found quite successful in the identification of Polystyrene (PS), Polyethylene terephthalate (PET), High Density Poly ethylene (HDPE), Low Density Poly ethylene (LDPE) etc. The Micro-Raman spectral data have been capable enough for the discrimination of high-density polyethylene (HDPE,> 0.940 g/cm3) and low-density polyethylene (LDPE,< 0.930 g/cm3) microplastics, especially due to the variations in the C-H stretching vibrations (2825-2970 cm−1). The ratio of intensities of asymmetric CH2 stretching mode (2879 cm−1) and the symmetric CH2 stretching mode (2847 cm−1) are found to be higher for HDPE with respect to LDPE. Moreover, intensity variations were also observed for the antisymmetric and symmetric C-C stretching bands present at 1059 cm−1 and 1126 cm−1 respectively. The band at 1167 cm−1 arising from CH vibration have also shown an intensity enhancement in HDPE samples.

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“…Tonicity is an essential factor for cells, which affects the metabolic activity and the exchange of substances with the extracellular region [1,2]. Changes in tonicity affect transmembrane ion transport by activating or inactivating specific ion channels [3], which is the characteristic of certain cells with tonicity imbalance due to disease [4].…”

Section: Introductionmentioning

confidence: 99%

Study of transmembrane ion transport under tonicity imbalance using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model

Li¹,

Kawashima²,

Sugawara³

et al. 2022

Biomed. Phys. Eng. Express

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Transmembrane ion transport under tonicity imbalance has been investigated using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model, by considering the cell diameter d [m] and the initial intracellular ion concentration cin [mM] as a function of tonicity expressed by sucrose concentration cs [mM]. The transmembrane ion transport is influenced by extracellular tonicity conditions, leading to a facilitation/inhibition of ion passage through the cell membrane. The transmembrane transport coefficient P [m/s], which represents the ability of transmembrane ion transport, is calculated by the extracellular ion concentrations obtained by improved ion transport model and LF-EIS measurement. P is calculated as 4.11×10-6 and 3.44×10-6 m/s at cs of 10 and 30 mM representing hypotonic condition, 2.44×10-6 m/s at cs of 50 mM representing isotonic condition, and 3.68×10-6, 5.16×10-6 , 9.51×10-6, and 14.89×10-6 m/s at cs of 75, 100, 125 and 150 mM representing hypertonic condition. The LF-EIS results indicate that the transmembrane ion transport is promoted under hypertonic and hypotonic conditions compared to isotonic condition. To verify the LF-EIS results, fluorescence intensity F [-] of extracellular potassium ions is observed to obtain the temporal distribution of average potassium ion concentration within the region of 3.6 m from cell membrane interface cROI [mM]. The slopes of ∆cROI /cROI1 to time t are 0.0003, 0.0002, and 0.0006 under hypotonic, isotonic, and hypertonic conditions, where cROI1 denotes initial cROI , which shows the same tendency with LF-EIS result that is verified by the potassium ion fluorescence observation.

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Red blood cells under varying extracellular tonicity conditions: an optical tweezers combined with micro-Raman study

Cited by 10 publications

References 59 publications

Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections

Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections

Identification of Microplastics Using a Custom Built Micro-Raman Spectrometer

Study of transmembrane ion transport under tonicity imbalance using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model

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