Red Cell Structure and Its Breakdown

Ponder, Eric

doi:10.1007/978-3-662-25095-2

Cited by 41 publications

(25 citation statements)

References 145 publications

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“…The observed progression of shape changes corresponded to that observed by previous workers (Ponder, 1948b(Ponder, ,1955Danon, 1961;Rand, 1967) if the transitory, flowinduced shape changes are neglected. The cells were smooth biconcave disks in isotonic dextrose but gradually thickened as the extenlal solute concentration decreased.…”

Section: The Process Of Controlled Gradual Osmotic Hemolysis Earl Eventssupporting

confidence: 87%

Molecular sieving action of the cell membrane during gradual osmotic hemolysis

MacGregor¹

1977

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Section: The Process Of Controlled Gradual Osmotic Hemolysis Earl Eventssupporting

confidence: 87%

Molecular sieving action of the cell membrane during gradual osmotic hemolysis

MacGregor¹

1977

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“…However, decreased swelling or shrinking in all three types of cell does not necessarily reflect an altered physical state of intracellular hemoglobin. An increase in intracellular water bound to hemoglobin, a decrease in the activity of intracellular cations, or a rigid or leaky membrane could produce the same results (33), and any of these mechanisms could apply to CC cells.…”

Section: Discussionmentioning

confidence: 97%

“…It seems likely that some "precrystalline" state of the hemoglobin in CC cells is responsible for the observed increase in their rigidity. Abnormal physical states of hemoglobin have been described in coarsely crenated normal red cells (32) and at least two other types of red cell, the deoxygenated sickle cell and the rat red cell suspended in cold citrate solution (33).…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of Hemolytic Anemia in Homozygous Hemoglobin C Disease*

Charache¹,

Conley²,

Waugh³

et al. 1967

J. Clin. Invest.

119

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Abstract. Hemoglobin C is less soluble than hemoglobin A in red cells, in hemolysates, and in dilute phosphate buffer. Its relative insolubility may be explained by electrostatic interactions between positively charged f36-lysyl groups and negatively charged groups on adjacent molecules. Red cells from patients with homozygous hemoglobin C (CC) disease exhibit aberrant physical properties which suggest that the cells are more rigid than normal erythrocytes. They pass through membrane filters less readily than normal red cells do, and their viscosity is higher than that of normal cells. Differences from normal cells are exaggerated if mean corpuscular hemoglobin concentration (MCHC) is increased, by suspension in hypertonic salt solution. Increased rigidity of CC cells, by accelerating their fragmentation, may be responsible for formation of microspherocytes. These small dense cells are exceptionally rigid, and probably are even more susceptible to fragmentation and sequestration. Rigidity of CC cells can be attributed to a "precrystalline" state of intracellular hemoglobin, in which crystallization does not occur, although the MCHC exceeds the solubility of hemoglobin in hemolysates.

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“…Ponder (2) has called attention to many types of chemical and physical treatments which are capable of producing disc-sphere shape tranformations in erythrocytes. Nakao, Nakao, Yamazoe, and Yoshikawa (3,4) have demonstrated that depletion of erythrocyte ATP also produces the disc-sphere transformation and that the latter was reversible upon restoration of ATP levels by incubation with adenine plus inosine.…”

mentioning

confidence: 99%