Red fluorescence-emitting copper nanoparticles-assisted label-free bioprobe coupled with CRISPR/Cas12a for sensitive detection of T4 polynucleotide kinase

Zhang, Liang; Li, Xinyang; Chen, Chen; Fang, Xu; Xu, Guanhong; Wei, Fangdi; Yang, Jian Feng; Hu, Qin; Zou, Jianjun; Yao, Chen

doi:10.1016/j.jphotochem.2023.114974

Journal of Photochemistry and Photobiology A: Chemistry

2023

DOI: 10.1016/j.jphotochem.2023.114974

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Red fluorescence-emitting copper nanoparticles-assisted label-free bioprobe coupled with CRISPR/Cas12a for sensitive detection of T4 polynucleotide kinase

Liang Zhang

Xinyang Li

Chen Chen

et al.

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Cited by 2 publications

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Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

Nayak,

Kamble,

Shettar

et al. 2023

Applied Organom Chemis

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The implementation of green chemistry principles to nanotechnology is one of the primary topics in the interdisciplinary area of nanoscience, which is a field of research that is perpetually expanding. A method for synthesizing Cu2O nanoparticles from copper(II) salts in an adroit and tactical way by employing solid jaggery as an economical and readily available source of reducing and stabilizing agent has been established. The explicitly synthesized nanoparticles were also discovered to be effective catalysts for the azide‐alkyne Huisgen cycloaddition (click reaction) in an aqueous media as green solvent. The substrate scope entails synthesis of 1,4‐disubstituted 1,2,3‐triazoles with good to excellent yields. The formation of Cu2O NPs was corroborated by various physicochemical methods like UV‐Visible (UV–Vis), Fourier Transform Infrared Spectroscopy (FTIR), Dynamic Light Scattering (DLS), Zeta potential, Field Emission Scanning Electron Microscopy (FESEM), energy dispersive X‐ray spectroscopy, and X‐ray diffraction analyses. The Cu2O NPs were substantiated by evaluation using HeLa, MCF‐7, and MDAMB‐231 (IC50: 72.66 μg ml−1, 47.31, 63.44 μg ml−1) cancer cell lines and unveiled eminent anticancer activities. This study also enabled us to elucidate the effects of Cu2O NPs on wound healing and cell migration.

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Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

Nayak,

Kamble,

Shettar

et al. 2023

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

A novel HCR-CRISPR/Cas12a immunosensor for the sensitive detection of pesticide residues in animal-derived foods

Wang,

Du,

Wang

et al. 2024

Food Bioscience

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Red fluorescence-emitting copper nanoparticles-assisted label-free bioprobe coupled with CRISPR/Cas12a for sensitive detection of T4 polynucleotide kinase

Cited by 2 publications

References 54 publications

Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

A novel HCR-CRISPR/Cas12a immunosensor for the sensitive detection of pesticide residues in animal-derived foods

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Red fluorescence-emitting copper nanoparticles-assisted label-free bioprobe coupled with CRISPR/Cas12a for sensitive detection of T4 polynucleotide kinase

Cited by 2 publications

References 54 publications

Dexterous green synthesis of Cu2O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

Dexterous green synthesis of Cu2O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

A novel HCR-CRISPR/Cas12a immunosensor for the sensitive detection of pesticide residues in animal-derived foods

Contact Info

Product

Resources

About

Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications

Dexterous green synthesis of Cu₂O nanoparticles: An amenable catalyst for aqueous click reaction, antiproliferative, and wound healing applications