Red laser-mediated alterations in seed germination, growth, pigments and withanolide content of Ashwagandha [Withania somnifera (L.) Dunal]

Thorat, Sachin Ashok; Poojari, Poornima; Kaniyassery, Arya; Kiran, Kodsara Ramachandra; Satyamoorthy, Kapaettu; Mahato, Krishna Kishore; Muthusamy, Annamalai

doi:10.1016/j.jphotobiol.2021.112144

Cited by 13 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pre-sowing laser irradiation on seeds, is a technique that can influence the different stages of the plant such as germination, plant growth and development, tolerance to abiotic stress and resistance to diseases (Ali et al, 2020;AlSalhi et al, 2019;Podleśna et al, 2015;Prośba-Białczyk et al, 2013;Thorat et al, 2021). The use of laser irradiation treatments by controlled doses, allows to analyze the effects of different treatments.…”

Section: Introductionmentioning

confidence: 99%

Impact of pre-sowing red laser irradiation of corn seeds on quality and quantity of harvest yield

2023

View full text Add to dashboard Cite

As the population increases, more people need to be fed. In order to find a physical method that influence the corn production, this work is dedicated to evaluating the impact of pre-sowing red laser irradiation of corn (Zea mays L.) seeds on harvest yield. The aim was to analyze the influence of red laser radiation on corn seeds, on the quantity and quality of yield, on large-scale and open field production. It is hypothesized that at least one red laser irradiation treatment could improve corn crop yield. In radiation, a red laser diode at 660 nm with a power of 100 mW was used, two radiation densities were used (D1: 2 mW cm-2 and D2: 4 mW cm-2), applied during 4 exposure times (T1: 15, T2: 30, T3: 60 and T4: 120 s) and a control group without treatment (C). A random arrangement was used, in a 2 x 4 factorial scheme, totaling 8 treatments and control, with four replications. The data were subjected to an ANOVA and the means were compared with the Tukey test (HSD; p ≤ 0.05). The D2-T4 treatment produced the most significant impact concerning the control, improving yield by 19±1% (3 t ha-1), cob length 18±1%, cob diameter 22±3%, weight (16±2%) and corn kernels size (14±2%). We also find negative effects on yield, D2-T1 decreased crop yield around 16±1% (2.24 t ha-1). The results show a way to include this technique as a technique that can to increase or decrease corn yield. Keywords: Cob length; Maize; open field; Thousand kernel weight.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of pre-sowing red laser irradiation of corn seeds on quality and quantity of harvest yield

2023

View full text Add to dashboard Cite

show abstract

“…Though the positive influences of laser biostimulation on seed growth have been reported many times, research results around this topic lack consistency [7][8][9][34][35][36][37][38][39]. This may be attributed to the selection of different laser settings (such as wavelength, power, dosage, exposure time, etc.)…”

Section: Introductionmentioning

confidence: 99%

Effect of Laser Biostimulation on Germination of Sub-Optimally Stored Flaxseeds (Linum usitatissimum)

et al. 2022

View full text Add to dashboard Cite

Sub-optimal storage of grains could deteriorate seed germination and plant viability. Recent research studies have established that laser biostimulation of seeds could be used as a safe and sustainable alternative to chemical treatment for improving crop germination and growth. Herein, the efficacy of this novel technique is evaluated to see if poor germinability caused by sub-optimal storage of flaxseeds (Linum usitatissimum) could be reversed using laser biostimulation. Healthy flaxseeds were first subjected to sub-optimal storage conditions (30 °C for ten weeks) to degrade their germinability. Two low-cost lasers, including a single-wavelength red laser (659 nm) and a dual-wavelength green/infrared laser (531 and 810 nm (ratio ~10:1)) were then used on two groups viz. healthy (properly stored) and sub-optimally stored (artificially degraded (AD)) seeds and irradiated for 0 (control), 5, 10, and 15 min using total power densities of 7.8 and 6.2 mW/cm2, respectively. In the case of AD seeds, 5-min dual-wavelength laser treatment was found to be the most efficient setting as it improved the mean germination percentage, mean germination time, germination speed, germination rate index, wet weight, and dry weight by 29.3, 16.8, 24.2, 24.2, 15.7, and 20.6%, respectively, with respect to control samples. In the case of healthy seeds, dual-wavelength laser treatment could induce significant enhancement in seeds’ root length, wet weight, and dry weight (improved by 26, 23, and 8%, respectively) under 10 min of irradiation. On the other hand, the effect of applied red laser treatment was not very promising as it could only induce significant enhancement in the mean germination time of AD seeds (improved by 17%). Overall, this study demonstrates the potential of laser biostimulation in reversing the adverse effect of poor crop storage. We believe these findings could spur the development of a physical tool for manipulating seed germination and plant growth.

show abstract

“…Plant tissue culture has also shown a great potential for the rapid production of phytomedicines ( Singh et al, 2016 ). Several conventional and molecular experiments have been performed in W. somnifera for crop improvement, but none of them have used novel bioactive molecules to enhance growth and metabolite production in W. somnifera ( Poornima et al, 2019 ; Sharma et al, 2020 ; Srivastava and Sangwan, 2020 ; Thorat et al, 2021 ). Although several studies are available on the in vitro regeneration and the quantification of withanolides, withanolide biosynthetic pathway gene expression, distribution of metabolites during the vegetative and reproductive phases, and adventitious root formation have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Differential Gene Expression and Withanolides Biosynthesis During in vitro and ex vitro Growth of Withania somnifera (L.) Dunal

et al. 2022

Self Cite

View full text Add to dashboard Cite

Ashwagandha (Withania somnifera L. Dunal) is a medicinally important plant with withanolides as its major bioactive compounds, abundant in the roots and leaves. We examined the influence of plant growth regulators (PGRs) on direct organogenesis, adventitious root development, withanolide biosynthetic pathway gene expression, withanolide contents, and metabolites during vegetative and reproductive growth phases under in vitro and ex vitro conditions. The highest shooting responses were observed with 6-benzylaminopurine (BAP) (2.0 mg L–1) + Kinetin (KIN) (1.5 mg L–1) supplementation. Furthermore, BAP (2.0 mg L–1) + KIN (1.5 mg L–1) + gibberellic acid (GA3) (0.5 mg L–1) exhibited better elongation responses with in vitro flowering. Half-strength MS medium with indole-3-butyric acid (IBA) (1.5 mg L–1) exhibited the highest rooting responses and IBA (1.0 mg L–1) with highest fruits, and overall biomass. Higher contents of withaferin A (WFA) [∼8.2 mg g–1 dry weight (DW)] were detected in the reproductive phase, whereas substantially lower WFA contents (∼1.10 mg g–1 DW) were detected in the vegetative phase. Cycloartenol synthase (CAS) (P = 0.0025), sterol methyltransferase (SMT) (P = 0.0059), and 1-deoxy-D-xylulose-5-phosphate reductase (DXR) (P = 0.0375) genes resulted in a significant fold change in expression during the reproductive phase. The liquid chromatography-mass spectrometry (LC-MS) analysis revealed metabolites that were common (177) and distinct in reproductive (218) and vegetative (167) phases. Adventitious roots cultured using varying concentrations of indole-3-acetic acid (IAA) (0.5 mg L–1) + IBA (1.0 mg L–1) + GA3 (0.2 mg L–1) exhibited the highest biomass, and IAA (0.5 mg L–1) + IBA (1.0 mg L–1) exhibited the highest withanolides content. Overall, our findings demonstrate the peculiarity of withanolide biosynthesis during distinct growth phases, which is relevant for the large-scale production of withanolides.

show abstract

Red laser-mediated alterations in seed germination, growth, pigments and withanolide content of Ashwagandha [Withania somnifera (L.) Dunal]

Cited by 13 publications

References 51 publications

Impact of pre-sowing red laser irradiation of corn seeds on quality and quantity of harvest yield

Impact of pre-sowing red laser irradiation of corn seeds on quality and quantity of harvest yield

Effect of Laser Biostimulation on Germination of Sub-Optimally Stored Flaxseeds (Linum usitatissimum)

Differential Gene Expression and Withanolides Biosynthesis During in vitro and ex vitro Growth of Withania somnifera (L.) Dunal

Contact Info

Product

Resources

About