Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings

Alallaq, Sanaria; Ranjan, Alok; Brunoni, Federica; Novák, Ondřej; Lakehal, Abdellah; Bellini, Catherine

doi:10.3389/fpls.2020.586140

Cited by 40 publications

(27 citation statements)

References 65 publications

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“…The induction and outgrowth of ARs are complex processes regulated by phytohormones, among which are JA, ETH and AUX (Gutierrez et al, 2012; Verstraeten et al, 2014; Steffens & Rasmussen, 2016; Lakehal & Bellini, 2019; Lakehal et al, 2019; Alallaq et al, 2020; Lakehal et al, 2020). The potential involvement of these phytohormones in AR spacing of c4h mutants was investigated using corresponding signalling mutants, namely coi1‐21 (JA), e tr1‐3 (ETH) and tir1afb2afb3 (AUX).…”

Section: Resultsmentioning

confidence: 99%

Seedling developmental defects upon blocking CINNAMATE‐4‐HYDROXYLASE are caused by perturbations in auxin transport

Houari¹,

Beirs²,

Arents³

et al. 2021

New Phytologist

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Summary The phenylpropanoid pathway serves a central role in plant metabolism, providing numerous compounds involved in diverse physiological processes. Most carbon entering the pathway is incorporated into lignin. Although several phenylpropanoid pathway mutants show seedling growth arrest, the role for lignin in seedling growth and development is unexplored. We use complementary pharmacological and genetic approaches to block CINNAMATE‐4‐HYDROXYLASE (C4H) functionality in Arabidopsis seedlings and a set of molecular and biochemical techniques to investigate the underlying phenotypes. Blocking C4H resulted in reduced lateral rooting and increased adventitious rooting apically in the hypocotyl. These phenotypes coincided with an inhibition in AUX transport. The upstream accumulation in cis‐cinnamic acid was found to be likely to cause polar AUX transport inhibition. Conversely, a downstream depletion in lignin perturbed phloem‐mediated AUX transport. Restoring lignin deposition effectively reestablished phloem transport and, accordingly, AUX homeostasis. Our results show that the accumulation of bioactive intermediates and depletion in lignin jointly cause the aberrant phenotypes upon blocking C4H, and demonstrate that proper deposition of lignin is essential for the establishment of AUX distribution in seedlings. Our data position the phenylpropanoid pathway and lignin in a new physiological framework, consolidating their importance in plant growth and development.

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Section: Resultsmentioning

confidence: 99%

Seedling developmental defects upon blocking CINNAMATE‐4‐HYDROXYLASE are caused by perturbations in auxin transport

Houari¹,

Beirs²,

Arents³

et al. 2021

New Phytologist

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“…Moreover, far-red treated Eucalyptus globulus plants presented an increased carbohydrate content and rooted better ( Ruedell et al, 2015 ). In spruce, constant red light had a positive effect on rooting initiation of de-rooted seedlings, possibly by repressing the accumulation of the negative regulators jasmonate and cytokinins ( Alallaq et al, 2020 ). In eastern cottonwood ( P. deltoides ), the rooting of microcuttings grown in media with exogenous auxin (IBA), was better under fluorescent light than under light emitting diodes (LEDs) ( Hoffman et al, 2016 ).…”

Section: Environmental Factorsmentioning

confidence: 99%

Adventitious Rooting in Populus Species: Update and Perspectives

Bannoud

Bellini

2021

Front. Plant Sci.

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Populus spp. are among the most economically important species worldwide. These trees are used not only for wood and fiber production, but also in the rehabilitation of degraded lands. Since they are clonally propagated, the ability of stem cuttings to form adventitious roots is a critical point for plant establishment and survival in the field, and consequently for the forest industry. Adventitious rooting in different Populus clones has been an agronomic trait targeted in breeding programs for many years, and many factors have been identified that affect this quantitative trait. A huge variation in the rooting capacity has been observed among the species in the Populus genus, and the responses to some of the factors affecting this trait have been shown to be genotype-dependent. This review analyses similarities and differences between results obtained from studies examining the role of internal and external factors affecting rooting of Populus species cuttings. Since rooting is the most important requirement for stand establishment in clonally propagated species, understanding the physiological and genetic mechanisms that promote this trait is essential for successful commercial deployment.

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“…The negative role of CKs in adventitious rooting has also been confirmed in many plant species that rely on vegetative propagation ( Lakehal and Bellini, 2019 ). For example, exogenous application of CK can inhibit AR formation in Malus domestica (apple) and Picea abies (Norway spruce) cuttings ( Mao et al , 2019 ; Alallaq et al , 2020 ). Similarly, Ramírez-Carvajal et al (2009) found that overexpressing an active variant of type-B cytokinin RESPONSE REGULATOR 13 ( PtRR13 ) delayed AR formation in Populus tremula×Populus alba stem cuttings.…”

Section: Discussionmentioning

confidence: 99%

Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis

Dob

Lakehal

Novák

et al. 2021

Journal of Experimental Botany

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Adventitious rooting is a de novo organogenesis process that enables plants to propagate clonally and cope with environmental stresses. Adventitious root initiation (ARI) is controlled by interconnected transcriptional and hormonal networks, but there is little knowledge of the genetic and molecular programs orchestrating these networks. Thus, we have applied genome-wide transcriptome profiling to elucidate the profound transcriptional reprogramming events preceding ARI. These reprogramming events are associated with the downregulation of cytokinin (CK) signaling and response genes, which could be triggers for ARI. Interestingly, we found that CK free-base (iP, tZ, cZ and DHZ) content declined during ARI, due to downregulation of de novo CK biosynthesis and upregulation of CK inactivation pathways. We also found that MYC2-dependent jasmonate (JA) signaling inhibits ARI by downregulating the expression of the CYTOKININ OXIDASE/DEHYDROGENASE1 (CKX1) gene. We also demonstrated that JA and CK synergistically activate expression of RELATED to APETALA2.6 LIKE (RAP2.6L) transcription factor, and constitutive expression of this transcription factor strongly inhibits ARI. Collectively, our findings reveal that previously unknown genetic interactions between JA and CK play key roles in ARI

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Red Light Controls Adventitious Root Regeneration by Modulating Hormone Homeostasis in Picea abies Seedlings

Cited by 40 publications

References 65 publications

Seedling developmental defects upon blocking CINNAMATE‐4‐HYDROXYLASE are caused by perturbations in auxin transport

Seedling developmental defects upon blocking CINNAMATE‐4‐HYDROXYLASE are caused by perturbations in auxin transport

Adventitious Rooting in Populus Species: Update and Perspectives

Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis

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