Red Light Regulates Calcium-Activated Potassium Channels in <i>Mougeotia</i> Plasma Membrane

Lew, Roger R.; Serlin, Bruce S.; Schauf, C. L.; Stockton, Marsha E.

doi:10.1104/pp.92.3.822

Cited by 35 publications

(23 citation statements)

References 6 publications

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“…The concs, given are those tested. a TEA + also inhibits K + channels in Hydrodictyon africanum [2], Nitellaflexilis [16], and Mougeotia [10]. b Internal inhibitor concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Although IK+out recorded in different types of cells exhibits similar activation kinetics and has a comparable activation range [2][3][4][5][6][7], the degree of homology between the K ÷ channel proteins in protoplasts derived from different species and/or tissues is unclear. The range in unitary conductance, 3 to 65 pS [3][4][5][8][9][10] recorded for plant plasmalemma K ÷ channels, and the observation that multiple regulatory mechanisms (e.g. cytosolic Ca 2÷ [10,11] or abscisic acid [8,12]) can modulate K ÷ currents in some protoplasts, suggest that more than one type of K ÷ channel may be responsible for IK+out in these diverse cell systems.…”

Section: Introductionmentioning

confidence: 99%

“…The range in unitary conductance, 3 to 65 pS [3][4][5][8][9][10] recorded for plant plasmalemma K ÷ channels, and the observation that multiple regulatory mechanisms (e.g. cytosolic Ca 2÷ [10,11] or abscisic acid [8,12]) can modulate K ÷ currents in some protoplasts, suggest that more than one type of K ÷ channel may be responsible for IK+out in these diverse cell systems.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts

Ketchum

Poole

1990

FEBS Letters

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We investigated the sensitivity of the Ca2+-dependent K ÷ current, IK(Ca), present in corn protoplasts, to different K + channel blockers. &cca) was inhibited by external Cs + (10 mM), Ba 2+ (10 mM), and quinine (0.5 mM): reagents which block many types of outward-rectifying K+channels. In contrast 4-aminopyridine (5 mM), an inhibitor of delayed rectifier or inactivating K + currents, had no effect. Neither of the peptide toxins, apamin or charybdotoxin, specific for CaZ+-dependent K ÷ channels in animal cells, inhibited currents when used in the nanomolar concentration range. However, higher levels of charybdotoxin (10 pM) caused marked reduction of IKcca).

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“…The concs, given are those tested. a TEA + also inhibits K + channels in Hydrodictyon africanum [2], Nitellaflexilis [16], and Mougeotia [10]. b Internal inhibitor concentration.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts

Ketchum

Poole

1990

FEBS Letters

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“…Linearly polarized light vibrating perpendicularly to the cell axis was used in this case in order to detect only the Pfr-fraction which is active in chloroplast movement. Red light regulates calcium-activated potassium channels in the plasma membrane of Mougeotia (Lew et al, 1990).…”

Section: Redlfar-red Light Absorbing Photoreceptor: Phytochromementioning

confidence: 99%

Photoreceptors in Algae

Rüdiger¹,

López‐Figueroa

1992

Photochem & Photobiology

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“…Careful control of cell turgor enabled a small portion of plasma membranebound cytoplasm to be exposed. (1,11,17,19,21), and more recently, the technique has been used to investigate their regulation (7,10,15,16,18,20).…”

Section: Introductionmentioning

confidence: 99%

Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell

Taylor¹,

Brownlee²

1992

Plant Physiol.

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We used an ultraviolet laser to rupture a small region of cell wall of a polarized Fucus spiralis rhizoid cell and gained localized access to the plasma membrane at the growing apex. Careful control of cell turgor enabled a small portion of plasma membranebound cytoplasm to be exposed. (1,11,17,19,21), and more recently, the technique has been used to investigate their regulation (7,10,15,16,18,20).Removal of the cell wall to expose plasma membrane suitable for patch recording was predicted to be a major problem in the advancement of single-channel studies in plant cells by Takeda and coworkers (22). In all but a few cases, removal of the cell wall is achieved by enzymic treatment to produce a spherical protoplast. the low success rates have often proved unsuccessful (6), although recent improvements to the procedure of enzymically isolating protoplasts may increase the number of successful GU seals (4).Plant cell protoplasts do not retain the structural order or polarity of the parent cell. This problem is of particular importance in the study of ion channel activity, distribution, and regulation in polarizing and polarized plant cells. In Fucus zygotes, the plant cell wall is required for the axis of polarity to develop (13); protoplasts from young Fucus zygotes are, therefore, of limited use in a single-channel study of polarity because localized cell wall removal and exposure of the underlying plasmalemma is required. Furthermore, the extraction and purification of the enzymes required for cell wall removal in Fucus are complex and time consuming (12). Although mechanical or surgical techniques have been used to gain access to the plasmalemma of Chara (3, 14), these are not suitable for smaller and less robust cells.In this report, we describe a novel technique for localized removal of the cell wall of Fucus rhizoid cells using a UV laser. Under appropriate conditions of turgor, the tip of the rhizoid cell is exposed, enabling high resistance GO seals to be obtained on the plasmalemma. MATERIALS AND METHODS Growth of ZygotesThallus tips bearing mature conceptacles of the dioecious marine alga Fucus spiralis were collected, washed, and stored at 40C in the dark for 1 to 3 d before they were placed in FSW2 at 180C to release zygotes. Zygotes were washed and transferred into small plastic chambers with a glass coverslip base. Dishes were placed in unilateral white fluorescent light (50 ,Em-2 s-1) at 180C to promote polarization, and zygotes were used 2 to 3 d after release. Preparation of ZygotesDishes were placed on the stage of an inverted microscope (Nikon Diaphot, Nikon, Tokyo, Japan) and treated with

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Red Light Regulates Calcium-Activated Potassium Channels in Mougeotia Plasma Membrane

Cited by 35 publications

References 6 publications

Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts

Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts

Photoreceptors in Algae

Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell

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Red Light Regulates Calcium-Activated Potassium Channels in Mougeotia Plasma Membrane

Cited by 35 publications

References 6 publications

Pharmacology of the Ca2+‐dependent K+ channel in corn protoplasts

Pharmacology of the Ca2+‐dependent K+ channel in corn protoplasts

Photoreceptors in Algae

Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell

Contact Info

Product

Resources

About

Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts

Pharmacology of the Ca²⁺‐dependent K⁺ channel in corn protoplasts