Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation

Borowski, Rafael Gomes Von; Barros, Muriel Primon de; Silva, Denise Brentan; Lopes, Norberto Peporine; Zimmer, Karine Rigon; Staats, Charley Christian; Oliveira, Cristiane Bernardes de; Giudice, Emmanuel; Gillet, Reynald; Macêdo, Alexandre José; Gnoatto, Simone Cristina Baggio

doi:10.1016/j.ijpharm.2019.118872

Cited by 15 publications

(7 citation statements)

References 76 publications

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“…Another of these minor chemicals are lectins, a group of proteins with glycoside agglutination properties. Some lectins have been described in C. frutescens and C. annuum seeds and have been studied as possible antimicrobials and have yielded positive results against some fungi [28,64].…”

Section: Other Minor Compoundsmentioning

confidence: 99%

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CapsicumSeeds as a Source of Bioactive Compounds: Biological Properties, Extraction Systems, and Industrial Application

Echave¹,

Pereira²,

Carpena³

et al. 2020

Capsicum

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Recent research has substantially focused on residual subproducts containing chemical compounds with bioactive properties. Even though there are some culinary or medicinal uses of Capsicum seeds, there is still a seed mass waste from pepper processing. Many pepper leading producer countries generally lack the facilities and infrastructure required for such processing technologies and so, pepper seeds are usually either destroyed or employed as landfilling or as animal feed. This involves an inadvertent economic loss for producers as well as a detrimental environmental impact. However, there is a hidden potential within the pepper processing industry related to valorization of pepper seeds to obtain added value by-products and thus reduce generated waste. Pepper seeds are a good source of antioxidants, carotenoids, phenolic acids, flavonoids, and vitamins C, E, and A and are also rich in volatile compounds, among others. The unique alkaloids of this genus are capsaicinoids and capsainoids, which have been linked to many beneficial biochemical and pharmacological effects including anti-oxidative or anti-inflammatory activities. Other prominent bioactive compounds of peppers seeds include saponins, lectins, and polyunsaturated fatty acids. In this context, an overview of the biological properties, extraction systems, and possible industrial application of bioactive compounds of pepper whole fruit and seeds is presented.

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Section: Other Minor Compoundsmentioning

confidence: 99%

“…Furthermore, a recent study analyzed the antiadhesive capacity of natural peptides from C. baccatum var. pendulum on biofilms made by Staphylococcus epidermidis [64]. Considering that biofilms are a source of significant virulence, new studies on this field present extremely interesting.…”

Section: Antimicrobialmentioning

confidence: 99%

CapsicumSeeds as a Source of Bioactive Compounds: Biological Properties, Extraction Systems, and Industrial Application

Echave¹,

Pereira²,

Carpena³

et al. 2020

Capsicum

View full text Add to dashboard Cite

show abstract

“…We synthesized the following three peptides inspired by a natural antibiofilm fraction previously identified from Capsicum baccatum var. pendulum pepper seeds ( 25 ): P1 ( RVQSEEGEDQISQRE ), P2 ( RAEAFQTAQALPGLCRI ), and P3 ( RSCQQQIQQAQQLSSCQQYLKQ ). To find the most active one, we exposed these compounds to strong biofilm-forming S. epidermidis RP62A (ATCC 35984).…”

Section: Resultsmentioning

confidence: 99%

Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm In Vitro and In Vivo via a Matrix Anti-Assembly Mechanism of Action

et al. 2021

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“…S. aureus and S. epidermidis are estimated to cause around 87% bloodstream, 40–50% prosthetic heart valve infection and 50–70% catheter biofilm infections ( Chen et al, 2013 ). Immense efforts has been dedicated to develop anti-biofilm coating on catheter and other medical devices to circumvent associated infections and post surgical complications in hospitalized patients ( Zhu et al, 2019 ; Von Borowski et al, 2020 ; Xie et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Sortase A Mediated Bioconjugation of Common Epitopes Decreases Biofilm Formation in Staphylococcus aureus

et al. 2020

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Staphylococcus aureus is one of the most notorious pathogens and is frequently associated with nosocomial infections imposing serious risk to immune-compromised patients. This is in part due to its ability to colonize at the surface of indwelling medical devices and biofilm formation. Combating the biofilm formation with antibiotics has its own challenges like higher values of minimum inhibitory concentrations. Here, we describe a new approach to target biofilm formation by Gram positive bacteria. Sortase A is a transpeptidase enzyme which is responsible for tagging of around ∼22 cell surface proteins onto the outer surface. These proteins play a major role in the bacterial virulence. Sortase A recognizes its substrate through LPXTG motif. Here, we use this approach to install the synthetic peptide substrates on S. aureus. Sortase A substrate mimic, 6His-LPETG peptide was synthesized using solid phase peptide chemistry. Incorporation of the peptide on the cell surface was measured using ELISA. Effect of peptide incubation on Staphylococcus aureus biofilm was also studied. 71.1% biofilm inhibition was observed with 100 µM peptide while on silicon coated rubber latex catheter, 45.82% inhibition was observed. The present work demonstrates the inability of surface modified S. aureus to establish biofilm formation thereby presenting a novel method for attenuating its virulence.

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Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation

Cited by 15 publications

References 76 publications

CapsicumSeeds as a Source of Bioactive Compounds: Biological Properties, Extraction Systems, and Industrial Application

CapsicumSeeds as a Source of Bioactive Compounds: Biological Properties, Extraction Systems, and Industrial Application

Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm In Vitro and In Vivo via a Matrix Anti-Assembly Mechanism of Action

Sortase A Mediated Bioconjugation of Common Epitopes Decreases Biofilm Formation in Staphylococcus aureus

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