Red pigment of adenine-deficient yeast Saccharomyces cerevisiae

Смирнов, М. Н.; Smirnov, V.N.; Budowsky, E.I.; Инге-Вечтомов, С. Г.; Serebrjakov, N.G.

doi:10.1016/s0006-291x(67)80096-2

Cited by 76 publications

(46 citation statements)

References 7 publications

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“…Yeasts were grown on YEPD medium to the late log phase. DNA was isolated from 10 g wet wt as in [3]. Further purification was performed by chromatography on hydroxyapatite columns (BioGel HT, Bio-Rad), equilibrated with K-P buffer.…”

Section: Methodsmentioning

confidence: 99%

“…Mutations in the ade-2 locus prevent the conversion of ($phosphoribosyl)aminoimidazole into its carboxylated (in the 4th position) derivative [2]. It is considered that the so-called 'red pigment', accumulated by ade-1 and ade-2 mutants, represents a polymeric form of this intermediate [3]. On investigating the ade-2 locus we encountered a problem of incomplete restriction of DNA, isolated from these mutants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases

Meškauskas¹,

Ksenzenko²,

Shlyapnikov³

et al. 1985

FEBS Letters

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases

Meškauskas¹,

Ksenzenko²,

Shlyapnikov³

et al. 1985

FEBS Letters

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show abstract

“…The Ade2 protein provides a convenient model, both because of its well-studied nature and because simple-selective and nonselective assays exist to monitor its activity. Ade2 is a phosphoribosylaminoimidazole carboxylase involved in the highly conserved de novo purine biosynthesis pathway (Smirnov et al 1967;Silver and Eaton 1969). Yeast cells lacking the Ade2 enzyme are unable to grow in the absence of adenine; in the presence of limiting adenine, cells lacking Ade2 activity turn red due to accumulation of a product derived from the substrate of Ade2 (Smirnov et al 1967;Silver and Eaton 1969).…”

Section: Weeks 4-7: Using Yeast To Study Enzyme Defectsmentioning

confidence: 99%

An Integrated Biochemistry and Genetics Outreach Program Designed for Elementary School Students

Ross

Lee²,

Radebaugh

et al. 2012

Genetics

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Exposure to genetic and biochemical experiments typically occurs late in one's academic career. By the time students have the opportunity to select specialized courses in these areas, many have already developed negative attitudes toward the sciences. Given little or no direct experience with the fields of genetics and biochemistry, it is likely that many young people rule these out as potential areas of study or career path. To address this problem, we developed a 7-week (1 hr/week) hands-on course to introduce fifth grade students to basic concepts in genetics and biochemistry. These young students performed a series of investigations (ranging from examining phenotypic variation, in vitro enzymatic assays, and yeast genetic experiments) to explore scientific reasoning through direct experimentation. Despite the challenging material, the vast majority of students successfully completed each experiment, and most students reported that the experience increased their interest in science. Additionally, the experiments within the 7-week program are easily performed by instructors with basic skills in biological sciences. As such, this program can be implemented by others motivated to achieve a broader impact by increasing the accessibility of their university and communicating to a young audience a positive impression of the sciences and the potential for science as a career.

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“…At two positions of this pathway, a faulty or missing enzyme will give rise to production of a red compound. This pigment is derived from an intermediate, 5Ј-phosphoribosylaminoimidazole (this compound is also known by the abbreviation AIR; Smirnov et al, 1967;Chaudhuri et al, 1996) that accumulates when the pathway is blocked due to defects in either the Ade1 or Ade2 proteins (Silver and Eaton, 1969). When intermediates AIR or the subsequent intermediate 5Ј-phosphoribosylaminoimidazole carboxylate (also known by the abbreviation CAIR) accumulate, AIR is oxidized to form a red polymer, which markedly shifts the color of Figure 1.…”

Section: Introductionmentioning

confidence: 99%

From Genes to Proteins to Behavior: A Laboratory Project That Enhances Student Understanding in Cell and Molecular Biology

Aronson

Silveira

2009

LSE

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In the laboratory, students can actively explore concepts and experience the nature of scientific research. We have devised a 5-wk laboratory project in our introductory college biology course whose aim was to improve understanding in five major concepts that are central to basic cellular, molecular biology, and genetics while teaching molecular biology techniques. The project was focused on the production of adenine in Saccharomyces cerevisiae and investigated the nature of mutant red colonies of this yeast. Students created red mutants from a wild-type strain, amplified the two genes capable of giving rise to the red phenotype, and then analyzed the nucleotide sequences. A quiz assessing student understanding in the five areas was given at the start and the end of the course. Analysis of the quiz showed significant improvement in each of the areas. These areas were taught in the laboratory and the classroom; therefore, students were surveyed to determine whether the laboratory played a role in their improved understanding of the five areas. Student survey data demonstrated that the laboratory did have an important role in their learning of the concepts. This project simulated steps in a research project and could be adapted for an advanced course in genetics.

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Red pigment of adenine-deficient yeast Saccharomyces cerevisiae

Cited by 76 publications

References 7 publications

‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases

‘Red pigment’ from ADE‐2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases

An Integrated Biochemistry and Genetics Outreach Program Designed for Elementary School Students

From Genes to Proteins to Behavior: A Laboratory Project That Enhances Student Understanding in Cell and Molecular Biology

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