Red Raspberry Polyphenols Attenuate High‐Fat Diet–Driven Activation of NLRP3 Inflammasome and its Paracrine Suppression of Adipogenesis via Histone Modifications

Fan, Rong; You, Mikyoung; Toney, Ashley Mulcahy; Kim, Judy; Gourichon, David; Xian, Yibo; Feng, Yan; Gu, Liwei; Ramer‐Tait, Amanda E.; Chung, Soonkyu

doi:10.1002/mnfr.201900995

Cited by 25 publications

(17 citation statements)

References 52 publications

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“…The extract was administrated to C57BL/6 mice revealing the attenuation of NLRP3 inflammasome activation in adipose tissue macrophages and epididymal white adipose tissue. The downregulation of NLRP3 inflammasome activation, leading to reduced expression levels of IL-1β, IL-18, and NLRP3 proteins, was also observed in vitro on C3H10T1/2 cells [ 92 ].…”

Section: Food Components As Nlpr3 Modulatorsmentioning

confidence: 99%

Modulatory Properties of Food and Nutraceutical Components Targeting NLRP3 Inflammasome Activation

et al. 2022

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Inflammasomes are key intracellular multimeric proteins able to initiate the cellular inflammatory signaling pathway. NLRP3 inflammasome represents one of the main protein complexes involved in the development of inflammatory events, and its activity has been largely demonstrated to be connected with inflammatory or autoinflammatory disorders, including diabetes, gouty arthritis, liver fibrosis, Alzheimer’s disease, respiratory syndromes, atherosclerosis, and cancer initiation. In recent years, it has been demonstrated how dietary intake and nutritional status represent important environmental elements that can modulate metabolic inflammation, since food matrices are an important source of several bioactive compounds. In this review, an updated status of knowledge regarding food bioactive compounds as NLRP3 inflammasome modulators is discussed. Several chemical classes, namely polyphenols, organosulfurs, terpenes, fatty acids, proteins, amino acids, saponins, sterols, polysaccharides, carotenoids, vitamins, and probiotics, have been shown to possess NLRP3 inflammasome-modulating activity through in vitro and in vivo assays, mainly demonstrating an anti-NLRP3 inflammasome activity. Plant foods are particularly rich in important bioactive compounds, each of them can have different effects on the pathway of inflammatory response, confirming the importance of the nutritional pattern (food model) as a whole rather than any single nutrient or functional compound.

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Section: Food Components As Nlpr3 Modulatorsmentioning

confidence: 99%

Modulatory Properties of Food and Nutraceutical Components Targeting NLRP3 Inflammasome Activation

et al. 2022

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“…Our study uniquely evaluated a polyphenolic extract from whole red raspberries that was stripped of any sugars and fibers capable of conferring confounding biological activities. Moreover, our extract contained all of the major polyphenols found in whole red raspberries, including quercetin, myricetin, EA, and anthocyanins, which enabled us to test the neuroprotective effects of whole fruit polyphenols rather than focusing on a concentrated form of EA, as has been studied previously [ 21 ]. Although RRW provided robust protection against neuroinflammation in LPS/ATP-stimulated microglia, RRW was only partly effective in reducing pro-inflammatory cytokine expression during 12 and 24 h LPS exposure, and it had no significant impact on JNK signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Freshly frozen “Wakefield” red raspberries were donated by Enfield Farms (Lynden, WA) and were used for this study. Extraction of red raspberry polyphenols from whole fruits (i.e., RRW) was conducted using a protocol previously described by our laboratory [ 21 ]. Phenolic composition of RRW extracts is found in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…To investigate the role of RRW and UroA in microglial inflammation, BV-2 cells were pretreated with either 10 µg/mL RRW or 10 µM UroA for 12 h. Cells were then subjected to either lipopolysaccharide (LPS, 500 ng/mL) treatment for 3 h followed by ATP (2.5 mM) stimulation for 30 min in Opti-MEM (Gibco) or LPS (500 ng/mL) treatment for 12 or 24 h. Concentrations of RRW polyphenols (10 μg/mL) and UroA (10 μM) were selected because they have been previously shown to induce beneficial responses in BV-2 microglia [ 17 ] as well as J774 and bone marrow-derived macrophages (BMDM) [ 21 ]. To investigate the effects of RRW and UroA on M2 polarization, BV-2 cells were pre-treated with either RRW (10 μg/mL) or UroA (10 μM) for 12 h and then stimulated with interleukin (IL)-4 and IL-13 (Peprotech, Cranbury, NJ, USA) in Opti-MEM for 24 h.…”

Section: Methodsmentioning

confidence: 99%

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Differential Effects of Whole Red Raspberry Polyphenols and Their Gut Metabolite Urolithin A on Neuroinflammation in BV-2 Microglia

Toney

Albusharif

Works

et al. 2020

IJERPH

Self Cite

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Whole red raspberry polyphenols (RRW), including ellagic acid, and their gut-derived metabolite, urolithin A (UroA), attenuate inflammation and confer health benefits. Although results from recent studies indicate that polyphenols and UroA also provide neuroprotective effects, these compounds differ in their bioavailability and may, therefore, have unique effects on limiting neuroinflammation. Accordingly, we aimed to compare the neuroprotective effects of RRW and UroA on BV-2 microglia under both 3 h and 12 and 24 h inflammatory conditions. In inflammation induced by lipopolysaccharide (LPS) and ATP stimulation after 3 h, RRW and UroA suppressed pro-inflammatory cytokine gene expression and regulated the JNK/c-Jun signaling pathway. UroA also reduced inducible nitric oxide synthase gene expression and promoted M2 microglial polarization. During inflammatory conditions induced by either 12 or 24 h stimulation with LPS, UroA—but not RRW—dampened pro-inflammatory cytokine gene expression and suppressed JNK/c-Jun signaling. Taken together, these results demonstrate that RRW and its gut-derived metabolite UroA differentially regulate neuroprotective responses in microglia during 3 h versus 12 and 24 h inflammatory conditions.

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“…The best sources of polyphenols to enhance AMPK activity will come from polyphenol concentrates of the anthocyanin family. These polyphenols can enhance the allosteric activation of sirtuins to indirectly activate AMPK ( 55 ) and inhibit inflammasome formation ( 122 , 123 ). Such polyphenol concentrates should also be devoid of glucose that would otherwise impede the activation of AMPK.…”

Section: Introductionmentioning

confidence: 99%

Dietary Control of Inflammation and Resolution

Sears

Saha

2021

Front. Nutr.

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The healing of any injury requires a dynamic balance of initiation and resolution of inflammation. This hypothesis-generating review presents an overview of the various nutrients that can act as signaling agents to modify the metabolic responses essential for the optimal healing of injury-induced inflammation. In this hypothesis-generating review, we describe a defined nutritional program consisting of an integrated interaction of a calorie-restricted anti-inflammatory diet coupled with adequate levels of omega-3 fatty acids and sufficient levels of dietary polyphenols that can be used in clinical trials to treat conditions associated with insulin resistance. Each dietary intervention works in an orchestrated systems-based approach to reduce, resolve, and repair the tissue damage caused by any inflammation-inducing injury. The orchestration of these specific nutrients and their signaling metabolites to facilitate healing is termed the Resolution Response. The final stage of the Resolution Response is the activation of intracellular 5' adenosine monophosphate-activated protein kinase (AMPK), which is necessary to repair tissue damaged by the initial injury-induced inflammation. The dietary optimization of the Resolution Response can be personalized to the individual by using standard blood markers. Once each of those markers is in their appropriate ranges, activation of intracellular AMPK will be facilitated. Finally, we outline how the resulting activation of AMPK will affect a diverse number of other intercellular signaling systems leading to an extended healthspan.

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Red Raspberry Polyphenols Attenuate High‐Fat Diet–Driven Activation of NLRP3 Inflammasome and its Paracrine Suppression of Adipogenesis via Histone Modifications

Cited by 25 publications

References 52 publications

Modulatory Properties of Food and Nutraceutical Components Targeting NLRP3 Inflammasome Activation

Modulatory Properties of Food and Nutraceutical Components Targeting NLRP3 Inflammasome Activation

Differential Effects of Whole Red Raspberry Polyphenols and Their Gut Metabolite Urolithin A on Neuroinflammation in BV-2 Microglia

Dietary Control of Inflammation and Resolution

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