Red rot resistant gene characterization using RGAP markers among sugarcane cultivars resistant and susceptible to the red rot disease

Abstract

Sugarcane is the major source of sugar in Asia and Europe, grown primarily in the tropical and sub-tropical zones of the world. The main disease responsible for its low yield is red rot. Therefore, in the present study, characterization of red rot disease was performed among 55 different sugarcane cultivars varying in red rot resistance level. 18 fragments were found to be associated with red rot resistance and were identified as resistant specific markers. The resistant specific fragments were amplified by RGA169, RGA396, RGA129, RGA231, RGA251, RGA057, RGA118, RGA152, RGA327, RGA542, RGA012, RGA173, RGA184, RGA275, RGA019, RGA267, RGA281 and RGA533. 7 fragments were found to be associated with red rot susceptibility and were considered as susceptible specific markers amplified by RGA088, RGA162, RGA396, RGA231, RGA251, RGA087 and RGA275. Sequencing of five resistant fragments, viz., RGA169, RGA231, RGA251, RGA267 and RGA533 was performed and the data thus obtained showed 80-99% similarity when compared with other resistant gene sequences previously submitted in NCBI database.

Occurrence and field evaluation of promising sugarcane genotypes for resistance to midrib red rot disease in China

“…In contrast, 64 susceptible varieties were categorized as susceptible (susceptible and highly susceptible), including ZZ9, ZZ6 and YR03‐425, with maximum disease incidence and index exceeding 70% and 50%, respectively. In this study, the percentage of HR genotypes was only 8.4%, indicating the critical need to develop resistant varieties (Sharma & Tamta, 2017). Previous studies demonstrated that a significantly high proportion of resistant progenies was obtained when both parents were resistant.…”

Colletotrichum falcatum e complexo de espécies de Fusarium spp. na cana-de-açúcar: caracterização das doenças e estratégias de controle

“…A doença é causada pelo fungo Colletotrichum falcatum (Glomerella tucumanensis) e é facilmente reconhecida com base nas características de seus sintomas de podridão e vermelhidão dos tecidos do caule. C. falcatum ocorre na natureza com enorme diversidade de patogenicidade e podem estar a evoluindo a partir das variantes prevalecentes em campo com um ganho significativo em sua patogenicidade e virulência devido à adaptação às novas variedades hospedeiras ou a um novo ambiente (Sharma et al, 2017).…”