Red-Skin Extracts of Lotus Seeds Alleviate High-Fat-Diet Induced Obesity via Regulating Lipoprotein Lipase Activity

Abstract

In recent years, obesity has become an epidemic and an important public health concern. This study was designed to explore the anti-obesity effects of red-skin extracts (RSE) from lotus seeds on high-fat-diet (HFD)-fed mice. In this study, a total of 55 phenolic compounds from the RSE were tentatively characterized using a UPLC-Q/TOF-MS system, including 9 phenolic acids and derivatives, 40 flavonoids, 2 proanthocyanidin, and 4 coumarins and derivatives. Our data demonstrated that RSE could significantly ameliorate obesity characteristics of HFD-fed mice by regulating tissue specific lipoprotein lipase (LPL) activities. In detailed, the activity and expression of LPL in adipose tissue was inhibited, and the activity and expression of LPL in skeletal muscle tissue was enhanced. Overall, these findings suggested that RSE from the red skin of lotus seeds could serve as a great candidate for a value-added, functional ingredient due to its anti-obesity effects via the regulation of LPL activity.

Hypoglycemic activity in vivo and in vitro of the Lotus (Nelumbo nucifera Gaertn.) seed skin (testa) phenolic-rich extracts

“…The potential of C. citratus as a medicinal plant for the treatment of diabetes revealed that its flavonoid glycosides have a hypoglycemic effect and can be explored as drug candidates to act as α-glucosidase inhibitors in the treatment of diabetes ( Borges et al, 2021 ). A total of 55 phenolic compounds of the red-skin extracts (RSE) from lotus seeds were identified or tentatively characterized, including 40 flavonoids, 9 phenolic acids, and derivatives, 4 coumarins and derivatives, and 2 proanthocyanidins ( Xu et al, 2022 ). In this study, a total of 38 phenolic compounds were first quantified in LSSE.…”

“…Phenolic composition of LSSE was quantified according to the method of Xu, Gao, Liu, and Gong (2022) with some modification. The Waters Acquity UPLC system equipped with an Acquity UPLC HSS T3 Column (1.8 μm, 2.1 mm × 100 mm) was used.…”

Chemical Profiling of Nitraria roborowskii Kom. by UPLC‐Q‐Orbitrap‐MS and Their Hypolipidemic Effects in Vivo

“…[16] Xu et al demonstrated that redskin phenolic compounds, represented by anthocyanins, flavanols, and flavonols, could significantly ameliorate obesity characteristics of HFD-fed mice by regulating tissue-specific lipoprotein lipase (LPL) activities. [49,50] In addition, the gene of LPL, which is involved in lipid metabolism and lipids trafficking, was down-regulated and may contribute to the atheroprotective effect of catechin. [51] Yang et al observed and investigated the effects and mechanisms of the pure total flavonoids from Citrus changshan-huyou on blood lipid metabolism in hyperlipidemic rats, and further mechanism investigation indicated that the pure total flavonoids from Citrus changshan-huyou inhibited serum lipid accumulation by increasing the expression LPL protein of the liver tissues.…”

“…Xu et al. demonstrated that red‐skin phenolic compounds, represented by anthocyanins, flavanols, and flavonols, could significantly ameliorate obesity characteristics of HFD‐fed mice by regulating tissue‐specific lipoprotein lipase (LPL) activities [49,50] . In addition, the gene of LPL, which is involved in lipid metabolism and lipids trafficking, was down‐regulated and may contribute to the atheroprotective effect of catechin [51] .…”

Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice

“…Moreover, studies have shown that the expression and activity of LPL vary in different tissues. The expression of LPL is upregulated in epididymal white adipose tissues (EWAT) in the HFD model, 58,59 but in retroperitoneal white adipose tissues (RWAT), LPL activity decreased significantly. There was no significant change in mRNA levels.…”