Redefining the photo-stability of common fluorophores with triplet state quenchers: mechanistic insights and recent updates

Abstract

Various strategies for TSQ-induced fluorophore stabilization and their application in sm-FRET as well as in super-resolution imaging microscopy are thoroughly reviewed.

Micro RNA Sensing with Green Emitting Silver Nanoclusters

“…The nanoclusters resemble molecular-like behavior with strong fluorescence observed in the wide range of the spectrum, from UV to visible to near IR [1,2]. AgNCs are more stable in photobleaching than widely used organic dyes, quantum dots, or fluorescent proteins [3][4][5]. These properties make AgNCs suitable for a plethora of practical applications including luminescent labelling, biological imaging, sensing, and catalysis [6][7][8].…”

Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging

“…15 This strategy was originally proposed to minimize photobleaching in dye lasers, 55 singlemolecule imaging and uorescence resonance energy transfer (FRET) and has been applied to time-lapse imaging of protein targets in xed and live cells. [16][17][18][19] Blanchard and coworkers have further carried out researches showing decreased ROS generation of TSQ-conjugated dyes, suggesting applications towards reducing phototoxicity in biological conditions. 20,21 We envision that this promising strategy, if proven compatible inside live cells, could benet live-cell uorescence analysis in general, such as in SR imaging and cytometry.…”

Establishing empirical design rules of nucleic acid templates for the synthesis of silver nanoclusters with tunable photoluminescence and functionalities towards targeted bioimaging applications

“…DNA-AgNCs are highly biocompatible, possess generally good photostability, are easy to synthesize using environmentally-benign reagents, and possess high versatility for tuning their photophysical properties, including photoemission color and brightness, across the entire range of the visible and near-IR spectrum by simply varying the nucleotide sequences of the DNA templates. They are important alternatives to traditional organic uorophores and inorganic quantum dots as organic uorophores oen require multi-stepped chemical syntheses and are susceptible to photobleaching, 56 while quantum dots can pose cytotoxicity concerns. 57,58 A huge repertoire of AgNCs with widely varying photophysical properties are currently known, 35 with those templated by poly-cytosine sequences 59 being arguably the most thoroughly studied.…”