Rederivation by embryo transfer in strains of laboratory mice and rats

Amstislavsky, S. Ya.; Igonina, T. N.; Рожкова, И. Н.; Brusentsev, E. Yu.; Rogovaya, A. A.; Ragaeva, D. S.; Naprimerov, V. A.; Litvinova, Ekaterina A.; Plyusnina, I. F.; Al, Markel'

doi:10.1134/s2079059713040023

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…In essence, GRBs are “repositories” offering the possibility of recreating breeds or breeding lines in case they are lost. Some examples of the re-establishment of populations have been described in polytocous species such as mice, rats, and rabbits [ 18 , 19 ]. Nonetheless, one of the most recurrent issues was related to how long cryopreserved embryos can be stored for [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Successful re-establishment of a rabbit population from embryos vitrified 15 years ago: The importance of biobanks in livestock conservation

2018

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Genetic resource banks (GRB) are a valuable tool for maintaining genetic variability and preserving breeds from pathogens or catastrophe, enabling us to assess and correct breeding schemes, minimizing the impact of genetic drift and facilitating diffusion. This study tests their efficiency in re-establishing two extinct populations of a synthetic rabbit line selected for daily weight gain, using vitrified embryos from two generations (18th and 36th) separated by 15 years of genetic selection. The effect of long-term storage of vitrified embryos in liquid nitrogen was also evaluated. A total of 516 vitrified embryos using the same protocol were transferred into 54 recipients. The embryos had been maintained in liquid nitrogen during 2 different periods, (i) 1 year (301 embryos and 26 transfers, 36th generation) and (ii) 15 years (259 embryos and 28 transfers, 18th generation). A total of 80.0% (8/10 to 18th) and 60.0% (9/15 to 36th) of the foundational sire families were eventually re-established. Over approximately one year, animals within each population were crossed to produce the next generation and re-establish the original population size. Our study demonstrated that our GRB of embryos vitrified 15 years ago is a successful strategy to re-establish rabbit populations to continue the breeding programme.

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Section: Introductionmentioning

confidence: 99%

Successful re-establishment of a rabbit population from embryos vitrified 15 years ago: The importance of biobanks in livestock conservation

2018

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“…Rederivation by embryo transfer demonstrated to be an efficient technique for eliminating infectious agents previously found in animals maintained in the quarantine area. The results obtained in our practice may be compared to what has been found at other international animal facilities to obtain SPF rodents (Van Keuren & Saunders, 2004;Amstislavsky et al, 2013).…”

Section: Discussionmentioning

confidence: 76%

Decontamination of genetically modified mice strains by embryo transfer for obtaining SPF colonies in a Brazilian animal facility

Antiorio¹,

Massironi²,

Luca³

et al. 2019

Braz. J. Vet. Res. Anim. Sci.

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The introduction of new strains of mice in specific pathogen-free (SPF) animal facilities should be performed carefully to avoid breaking sanitary barriers. To meet this need, animals should be rederived to reduce infection risk and thus avoid research interference caused by loss of animal health status and welfare. The objective of this study was to implement mice embryo transfer in the laboratory mouse facility of the Department of Immunology at the Institute of Biomedical Sciences/University of São Paulo, Brazil. Embryo transfers were performed to rederive genetically modified mouse strains with undefined sanitary status, received from different research and educational institutions. Fertilized eggs at two-cell stage were obtained by natural means and transferred into the oviducts of SPF pseudo-pregnant female mice. All surgical procedures were performed under aseptic conditions. A total of 625 embryos were transferred into therecipients. 148 pups were born, of which 140 were reared. Viruses, bacteria and intestinal protozoa were eliminated using this technique. The improvement in the microbiological status of mice allowed their expansion in our SPF facility. With these results, we can stimulate the use of embryo transfer technique between rodent facilities in Brazil and thus encourage the distribution of better models to our scientific community.

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“…For rederivation purposes, superovulation is used to increase the number of oocytes released by each female that can then be collected after natural mating at 0.5 dpc (1-cell embryos) [6], at 1.5 dpc (2-cells embryos) [7] or even at 3.5 dpc (blastocysts) [8] for subsequent embryo transfer. Embryo transfer can then be performed at several stages of embryo development using 1-cell embryo [6] [9], 2-cells embryos [6] [7] [9] or blastocysts [8] [10]. Embryo culture can also be part of the process by collecting embryos at 0.5 dpc and cultivating up to a 2-cells or blastocyst stage.…”

Section: Introductionmentioning

confidence: 99%

Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

Lamas¹,

Franquinho²,

Morgado³

et al. 2020

OJAS

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Superovulation is used to stimulate the production and release of large amounts of oocytes in mice by using two hormones that mimic FSH (PMSG) and LH (hCG) effects. Since superovulation can have a negative impact on oocyte and embryo development, this investigation aimed to compare two alternatives for 2-cells embryo collection in order to reduce the number of females and to benefit from the superovulation process. Data from mouse embryo collection from our facility was analyzed to compare the number of 2-cells embryos collected at 1.5 dpc and the number of 2-cells embryos obtained after overnight incubation of 1-cell embryos, collected at 0.5 dpc. Genetically modified mouse strains with a similar background (C57BL/6J, B6*129 and FVB/NJ) were analyzed and for strains at a C57BL/6J and B6*129 background, the number of 2-cells embryos obtained after incubation was significantly higher when compared to the number of 2-cells embryos collected at 1.5 dpc (1.4-fold and 1.7-fold, respectively). C57BL/6J wild type mice had similar results with a higher number of 2-cells embryos when collection was performed at 0.5 dpc followed by incubation (1.4-fold). These results can help the planning of 2-cells embryo harvesting by reducing the number of females needed for this procedure.

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Rederivation by embryo transfer in strains of laboratory mice and rats

Cited by 10 publications

References 44 publications

Successful re-establishment of a rabbit population from embryos vitrified 15 years ago: The importance of biobanks in livestock conservation

Successful re-establishment of a rabbit population from embryos vitrified 15 years ago: The importance of biobanks in livestock conservation

Decontamination of genetically modified mice strains by embryo transfer for obtaining SPF colonies in a Brazilian animal facility

Harvesting of Mouse Embryos at 0.5 Dpc as a Tool to Reduce Animal Use: Data from C57BL/6J, B6*129 and FVB/NJ Strains

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