Redescription of Tanqua tiara (von Linstow, 1879) Blanchard, 1904 (Nematoda: Gnathostomatidae) from Varanus flavescens (Hardwicke and Gray, 1827) (Reptilia: Varanidae) from Birbhum district, West Bengal, India

Abstract

Tanqua tiara (von Linstow, 1879) Blanchard, 1904, recovered from the stomach of water monitor lizard, Varanus flavescens (Hardwicke and Gray, 1827), collected from the Dubrajpur town, Birbhum district of West Bengal, India, is redescribed with light as well as scanning electron microscopy. This species is characterised by presence of two large lateral pseudolabia followed by massive cephalic bulb. The inner surface of each pseudolabium is folded into five rounded tooth-like formations which fit into corresponding folds on adjacent pseudolabium. Spicules are equal and similar. Gubernaculum is absent. Caudal papillae are sessile and five pairs in number. Present nematode differs from earlier descriptions of T. tiara in having five pairs of caudal papillae and an unpaired median precloacal papilla. Position of phasmids has also been reported in present study. Actual structure of cervical papillae and cuticular striations have been shown in present SEM studies which are also different from earlier descriptions. Dubrajpur is recorded as new geographical area for this parasite.

Genetic characterisation of Tanqua (von Linstow, 1879) (Nematoda: Gnathostomatidae) larval forms including new host and locality records

“…Twelve of the specimens in the present study used for DNA extraction were entire, and although internal structures were damaged ( Fig. 2 A) the cephalic region of the 12 entire specimens followed the description of the cephalic region of adult Tanqua tiara in Sou (2020) in which large lateral pseudolabia and tooth like projections were described. Fig.…”

“…However, all of the 12 specimens used for DNA extraction, despite having damage to the trunk and internal structures, had the head and tail intact. All of the 12 specimens were annulated over the entire body and had similar toothlike projections and large lateral pseudolabia described in adult T. tiara by Sou (2020) . Of the remaining 15 larval nematodes, not subjected to DNA extraction, and with undamaged cephalic regions the same features were identified.…”

“…6 B of the publication. The entire body, of all of the 12 specimens used for DNA extraction in the present study, were heavily annulated and although characteristic of a number of other genera of nematodes ( Moravec et al, 2007 ; Pereira et al, 2014 ; Velarde-Aguilar et al, 2015 ; Ocampo-Salinas et al, 2021 ), are also described in adult Tanqua tiara ( Sou, 2020 ). Specimens identified as Tanqua sp.…”

“…in Schoeman et al (2020) . Intraspecific morphological variations of adult T. tiara has been reported ( Sou, 2020 ). The specimens in the present study showed low genetic variability with adult T. tiara (1.8%: JF934728) in Laetsch et al (2012) and by L3 Tanqua sp.…”

Record on Nematode <I>Tanqua tiara</I> Infection on Snakehead Fish <I>Channa striata</I> in South Kalimantan Indonesia

“…Male T. tiara which infected V. salvator and V. magna was reported to have total length of 13.8 -28.6 mm with body width of 0.39-0.77 mm, while the female has total length of 13.0-25.7 mm with body width of 0.26-0.42 mm (Purwaningsih et al, 2020). Sou (2020) summarized that the male has total length of 13.50-39.00 mm with body width of 0.19-1.10 mm, while the Table 1. Alignment analysis on nucleotide of 18S rRNA from the nematode from Kandangan Lama with the data on the GenBank with indicated accession numbers female has total length of 20.00-45.46 mm with body width of 0.52-1.51 mm.…”