Rediscovery of the critically endangered ‘scarce yellow sally stonefly’ Isogenus nubecula in United Kingdom after a 22 year period of absence

Davy‐Bowker, John; Hammett, Michael J.; Mauvisseau, Quentin; Sweet, Michael

doi:10.11646/zootaxa.4394.2.12

Cited by 3 publications

(8 citation statements)

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“…Sampling was undertaken at the end of winter/beginning of spring, when environmental conditions such as high flow rates or flood events could have decreased and diluted the quantity of DNA traces. However, this was unavoidable for this species as I. nubecula emerges from March onwards 31,39 and so for this species sampling time could not be altered.…”

Section: Discussionmentioning

confidence: 98%

“…Finally, when sampling for any eDNA study, it is useful to have an understanding the ecology of the species under study, such as the species habits and preferred habitat in which it occurs. However, again, as I. nubecula was recently rediscovered in Wales, there is very little information on this species 32 . Our site occupancy modelling approach was also not able to identify any specific variable which would have a significant effect on the probability of detection of this species (Figure 3, Tables 3, 4), which is quite logic as all the sites were located in the same study system.…”

Section: Discussionmentioning

confidence: 99%

“…A typical example of such a bioindicator Plecoptera is the Scarce Yellow Sally stonefly, Isogenus nubecula (Perlodidae, Plecoptera) (Newman 1833). This critically endangered species has been reported as extinct or undetected in numerous countries from which it was originally present 31,32 . Also in the UK, it was considered as extinct until recently, when I. nubecula specimens were rediscovered after a 22 year period of absence at a location in the river Dee, North Wales, UK 32 .…”

Section: Introductionmentioning

confidence: 99%

“…This critically endangered species has been reported as extinct or undetected in numerous countries from which it was originally present 31,32 . Also in the UK, it was considered as extinct until recently, when I. nubecula specimens were rediscovered after a 22 year period of absence at a location in the river Dee, North Wales, UK 32 . Moreover, a total of 14 individuals were recorded on that spot during two kick-sampling surveys.…”

Section: Introductionmentioning

confidence: 99%

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Improving detection capabilities of a critically endangered freshwater invertebrate with environmental DNA using digital droplet PCR

Mauvisseau

Davy‐Bowker

Bulling

et al. 2019

Preprint

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21Isogenus nubecula is a critically endangered Plecoptera species. Considered extinct in the UK, the 22 species was recently rediscovered in one location of the river Dee in Wales after 22 years of absence. As 23 many species belonging to the Perlodidae, this species can be a bio-indicator, utilised for assessing 24 water quality and health status of a given freshwater system. However, conventional monitoring of 25 invertebrates via kick-sampling for example, is an invasive and expensive (time consuming). Further, 26 such methods require a high level of taxonomic expertise. Here, we compared the traditional kick-27 sampling method with the use of eDNA detection using qPCR and ddPCR-analyses. In spring 2018, we 28 sampled eDNA from twelve locations on the river Dee. I. nubecula was detected using kick-sampling in 29 five of these locations, three locations using both eDNA detection and kick-sampling and one location 30 using eDNA detection alone -resulting in a total of six known and distinct populations of this critically 31 endangered species. Interestingly, despite the eDNA assay being validated in vitro and in silico, and 32 results indicating high sensitivity, qPCR analysis of the eDNA samples proved to be ineffective. In 33 contrast, ddPCR analyses resulted in a clear detection of I. nubecula at four locations suggesting that 34 inhibition most likely explains the big discrepancy between the obtained qPCR and ddPCR results. It is 35 therefore important to explore inhibition effects on any new eDNA assay. We also highlight that ddPCR 36 may well be the best option for the detection of aquatic organisms which are either rare or likely to shed 37 low levels of eDNA into their environment. 38

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improving detection capabilities of a critically endangered freshwater invertebrate with environmental DNA using digital droplet PCR

Mauvisseau

Davy‐Bowker

Bulling

et al. 2019

Preprint

Self Cite

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“…A typical example of a bioindicator Plecoptera is the Scarce Yellow Sally stonefly, Isogenus nubecula (Perlodidae, Plecoptera) (Newman 1833). This critically endangered species has been reported as extinct or undetected in much of its historical home range 31,32 . I. nubecula is listed as a UK Biodiversity Action Plan priority species and a species of principal importance in Wales (www.nhm.ac.uk/our-science/data/uk-species/species/isogenus_nubecula) for example.…”

Section: Introductionmentioning

confidence: 99%

Combining ddPCR and environmental DNA to improve detection capabilities of a critically endangered freshwater invertebrate

Mauvisseau

Davy‐Bowker

Bulling

et al. 2019

Sci Rep

Self Cite

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Isogenus nubecula is a critically endangered Plecoptera species. Considered extinct in the UK, I. nubecula was recently rediscovered (in one location of the River Dee, Wales), after 22 years of absence. In a similar way to many other species of Perlodidae, I. nubecula could be utilised as a bio-indicator, for assessing water quality and health status of a given freshwater system. However, conventional monitoring of invertebrates via kick-sampling, is invasive and expensive (time consuming). Further, such methods require a high level of taxonomic expertise. Here, we compared the traditional kick-sampling method with the use of eDNA detection using qPCR and ddPCR-analyses. In spring 2018, we sampled eDNA from twelve locations on the River Dee. I. nubecula was detected using kick-sampling in five of these locations, three locations using both eDNA detection and kick-sampling and one location using eDNA detection alone – resulting in a total of six known and distinct populations of this critically endangered species. Interestingly, despite the eDNA assay being validated in vitro and in silico, and results indicating high sensitivity, qPCR analysis of the eDNA samples proved to be ineffective. In contrast, ddPCR analyses resulted in a clear detection of I. nubecula at four locations suggesting that inhibition most likely explains the large discrepancy between the obtained qPCR and ddPCR results. It is therefore important to explore inhibition effects on any new eDNA assay. We also highlight that ddPCR may well be the best option for the detection of aquatic organisms which are either rare or likely to shed low levels of eDNA into their environment.

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New Distributional Data for the Northern Forestfly, Lednia borealis Baumann and Kondratieff, 2010 (Plecoptera: Nemouridae), in Washington, USA

Fallon

Blevins

Blackburn

et al. 2022

Western North American Naturalist

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The northern forestfly, Lednia borealis (Plecoptera: Nemouridae) is a rare montane stonefly believed to be endemic to Washington. The species, first recognized as a valid taxon in 2010, is the only member of the genus Lednia known from the state. Like other species in its genus, it is found in mid-to high-elevation cold water habitat, including lakes, glacial-fed streams, and rheocrenes (channelized springs). Lednia species in general appear to be rare or at least rarely collected. Because of their reliance on alpine and subalpine habitat, Lednia may be especially vulnerable to threats associated with climate change. However, relatively little is known about this species, and distribution data are scarce. From 2015 to 2019, 94 sites were surveyed in order to document unmapped populations of Lednia borealis to improve range and distributional information from montane areas of Washington State. In this paper, we share locations of L. borealis documented to date, including collections from eight newly documented Lednia sites in the Mt. Baker and Glacier Peak Wildernesses in the Cascade Mountains of Washington, and report recent COI barcoding results. We also provide updated details on the species' distribution, highlight a confirmed habitat association with glacial edge meltwater, and provide recommendations for future surveys.

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Rediscovery of the critically endangered ‘scarce yellow sally stonefly’ Isogenus nubecula in United Kingdom after a 22 year period of absence

Cited by 3 publications

References 4 publications

Improving detection capabilities of a critically endangered freshwater invertebrate with environmental DNA using digital droplet PCR

Improving detection capabilities of a critically endangered freshwater invertebrate with environmental DNA using digital droplet PCR

Combining ddPCR and environmental DNA to improve detection capabilities of a critically endangered freshwater invertebrate

New Distributional Data for the Northern Forestfly, Lednia borealis Baumann and Kondratieff, 2010 (Plecoptera: Nemouridae), in Washington, USA

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