Redistribution of Lymphocytes Following E. coli Sepsis

Abstract

Infusion of endotoxin elicits lymphopenia and a transient granulocytopenia followed by granulocytosis in peripheral blood. The purpose of this study was to investigate which tissues the lymphocytes are redistributed to in response to endotoxaemia. Lymphocytes were isolated from the peripheral blood of 20 rabbits, labelled with 111Indium-tropolene and reinjected intravenously into the rabbits. Ten rabbits received an infusion of Escherichia coli endotoxin 2 micrograms/kg-1, while 10 rabbits received isotonic saline and served as a control group. The redistribution of lymphocytes was imaged with a gamma camera, and calculated with an interfaced computer before, and 2, 4 and 6 h after infusion of endotoxin or saline. Interleukin-1 beta and serum cortisol were measured. Following endotoxaemia the lymphocytes in peripheral blood decreased from 1.95 10(9)/l to 0.83 6 h later. Interleukin-1 beta and serum cortisol increased significantly. The radioactivity of labelled cells in the spleen and in the heart and lungs decreased to 83.3% and 87.8% of initial values respectively, 6 h after infusion of endotoxin. The radioactivity of the lymphatic tissue in and around the intestine increased to 128.8% of initial values. The results indicate that endotoxaemia induces redistribution of lymphocytes from peripheral blood and spleen to lymphatic tissue.

Clinical significance of changes in peripheral lymphocyte count after surgery in early cervical cancer

“…For example, circulating lymphocyte numbers fall peri-operatively [15][16][17], possibly due to a decrease in the lymphocyte proliferation rate [18,19] or a redistribution of lymphocytes from the peripheral blood to the body compartments [20]. We also found that peripheral lymphocyte counts fell significantly after surgery.…”

Up-regulation of functional CXCR4 expression on human lymphocytes in sepsis

“…Apoptosis and clearance from the circulation have been suggested in some experiments (31,32). There are also reports that lymphocytes redistribute from peripheral blood and spleen to mesenteric lymphoid tissue or accumulate in various organs (8,33). It is unknown why and into which tissues peripheral blood lymphocytes disappear.…”

“…For example, lymphocyte activation via superantigens induces systemic release of cytokines resulting in T-cell-dependent septic shock and severe lung injury (31). Alternatively, lymphocyte may indirectly cause damage following secretion of proinflammatory cytokines and chemokines (31)(32)(33)(34).…”

Retrospective Study of Tick-Borne Pathogens and Observation of <i>Ehrlichia ewingii / Anaplasma phagocytophilum</i> and Hemotropic <i>Mycoplasma</i> spp. in Dogs’ Blood Films

“…Bacterial infections, among other infectious diseases are more important for left shift occurrence [72,73]. Glucocorticoids increase lymphocyte sequestration and apoptosis [74][75][76], some secreted cytokines in bacterial and viral infections also cause lymphopenia [77][78][79]. Reactive lymphocytes are antigen stimulated lymphocytes which their size and their cytoplasmic basophilia were increased [73].…”