1996
DOI: 10.1007/bf02257274
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Redistribution of ribosomal DNA after blocking of transcription induced by actinomycin D

Abstract: We report on the effect of different doses and times of incubation of the cytostatic drug actinomycin D (AMD) on nucleolar morphology, rRNA gene transcription and rDNA gene localization using in situ hybridization and the immunocytochemical detection of the human upstream binding factor (UBF) at the electron microscopic level in HeLa cells. Low doses of AMD (0.001 micrograms/ml, 30 min) selectively block rRNA gene transcription but alter neither nucleolar morphology nor the localization of rDNA with respect to… Show more

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Cited by 26 publications
(22 citation statements)
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References 20 publications
(16 reference statements)
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“…It was shown previously that functional subcompartmentalisation is not necessarily reflected in the ultrastructural appearance of the DF (Kill 1996;Mosgoeller et al 1996;Schöfer et al 1996). All these findings and our present data indicate that within the DF, different functional domains for transcription and for , d), fluorescent in situ hybridisation using the 3′ probe targeting DNA (a, b) and RNA (c, d).…”
Section: Discussionsupporting
confidence: 68%
“…It was shown previously that functional subcompartmentalisation is not necessarily reflected in the ultrastructural appearance of the DF (Kill 1996;Mosgoeller et al 1996;Schöfer et al 1996). All these findings and our present data indicate that within the DF, different functional domains for transcription and for , d), fluorescent in situ hybridisation using the 3′ probe targeting DNA (a, b) and RNA (c, d).…”
Section: Discussionsupporting
confidence: 68%
“…In situ hybridization with specific rDNA probes often demonstrates the presence of rDNA in clumps of nucleolusassociated chromatin both at microscopic and ultrastructural levels (Thiry et al, 1988;Thiry and Thiry-Blaise, 1991;Kaplan et al, 1993). The retraction of rDNA out of the nucleolus into perinucleolar chromatin upon inhibition of transcription by actinomycin D (Schofer et al, 1996) confirms the idea that the balance between compacted (perinucleolar) and extended (intranucleolar) rDNA is correlated with the level of rRNA synthesis. Strong correlation between condensin and topoII localization, observed in nucleoli, implies the cooperative action of these proteins in transcription-related reconfiguration of the rDNA template (Kimura et al, 1999).…”
Section: Discussionmentioning
confidence: 79%
“…Under these conditions, the transcription by RNApol I is completely blocked (Schofer et al, 1996). Indeed, morphological changes in nucleoli in actinomycin-D-treated cells, which result in nucleolar segregation, clearly indicate that rRNA synthesis is severely affected (Figs 5-9).…”
Section: Levels Of Peg7 and Xcap-e Proteins Did Not Change Significanmentioning
confidence: 93%
“…However in the interphase of actinomycin D treated cells TRF2 was localized at the center of the nucleolus, whereas UBF was confined to the nucleolar boundary and away from TRF2. Moreover this effect excludes a longitudinal binding of TRF2 to rDNA, because actinomycin D not only led to the dissociation of UBF (Jordan et al, 1997) but also to a retraction of rDNA fibers to the nucleolar periphery (Schofer et al, 1996). Therefore, rather than spreading on route along rDNA fibers, TRF2 may help to build up complexes that abut on rDNA or its associated proteins, although spreading on route may occur on the TTAGGG telomeric sequence repeats.…”
Section: Discussionmentioning
confidence: 99%