Redistribution of synaptic AMPA receptors at glutamatergic synapses in the dorsal cochlear nucleus as an early response to cochlear ablation in rats

Rubio, María E.

doi:10.1016/j.heares.2006.03.007

Cited by 46 publications

(65 citation statements)

References 63 publications

(103 reference statements)

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“…Then mice were perfused transcardially with 4% PFA and 2% glutaraldehyde in PBS. Vibratome sections (200 m thick) were collected in 0.1 M PB buffer, pH 7.2, postfixed in 1% osmium tetroxide, dehydrated in ethanol, infiltrated, and flat embedded in epoxy as previously described (Rubio, 2006). CA1 hippocampi were trimmed and mounted on blocks for further semithin and ultrathin sectioning with a Leica Ultracut.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…To determine the area of spines, we traced the plasma membrane with ImageJ (http://rsb.info.nih.gov/ij) (Sfakianos et al, 2007). The average thickness of the PSDs was measured as described previously (Rubio, 2006). Briefly, the cytoplasmic outline of a PSD, including the associated dense material, was traced, and this area was then enclosed by tracing the postsynaptic membrane (length of PSD).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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The Planar Polarity Protein Scribble1 Is Essential for Neuronal Plasticity and Brain Function

Moreau

Piguel

Papouin³

et al. 2010

Journal of Neuroscience

103

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Scribble (Scrib) is a key regulator of apicobasal polarity, presynaptic architecture, and short-term synaptic plasticity in Drosophila. In mammals, its homolog Scrib1 has been implicated in cancer, neural tube closure, and planar cell polarity (PCP), but its specific role in the developing and adult nervous system is unclear. Here, we used the circletail mutant, a mouse model for PCP defects, to show that Scrib1 is located in spines where it influences actin cytoskeleton and spine morphing. In the hippocampus of these mutants, we observed an increased synapse pruning associated with an increased number of enlarged spines and postsynaptic density, and a decreased number of perforated synapses. This phenotype was associated with a mislocalization of the signaling pathway downstream of Scrib1, leading to an overall activation of Rac1 and defects in actin dynamic reorganization. Finally, Scrib1-deficient mice exhibit enhanced learning and memory abilities and impaired social behavior, two features relevant to autistic spectrum disorders. Our data identify Scrib1 as a crucial regulator of brain development and spine morphology, and suggest that Scrib1 crc/؉ mice might be a model for studying synaptic dysfunction and human psychiatric disorders.

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Section: Immunohistochemistrymentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

The Planar Polarity Protein Scribble1 Is Essential for Neuronal Plasticity and Brain Function

Moreau

Piguel

Papouin³

et al. 2010

Journal of Neuroscience

103

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“…Low glutaraldehyde fixation was followed by either pre-embedding immunocytochemistry or freeze-substitution as previously described (Rubio and Wenthold 1997;Tzounopoulos et al 2007). The identification at the electron microscope of cartwheel and fusiform cells and inhibitory synaptic endings on those neuronal types was done as previously described (Rubio 2006;Rubio and Juiz 2004;Tzounopoulos et al 2007). …”

Section: Electron Microscopymentioning

confidence: 99%

“…Both antibodies were rabbit polyclonal and were a gift from Dr. Ken Mackie. For the detection of DGL␣, DGL␤, CB1Rs, and GlyR␣1 receptor subunits, with immunogold labeling after freeze-substitution, a protocol similar to that described in detail elsewhere was used (Rubio 2006;Rubio andWenthold 1997, 1999). Sections at the level of the cochlear nucleus of two mice were dissected and processed for freeze-substitution and low-temperature embedding.…”

Section: Pre-embedding Immunohistochemistry and Freeze-substitution Amentioning

confidence: 99%

Distinct Functional and Anatomical Architecture of the Endocannabinoid System in the Auditory Brainstem

Zhao

Rubio²,

Tzounopoulos³

2009

Journal of Neurophysiology

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Zhao Y, Rubio ME, Tzounopoulos T. Distinct functional and anatomical architecture of the endocannabinoid system in the auditory brainstem. J Neurophysiol 101: 2434 -2446, 2009. First published March 11, 2009 doi:10.1152/jn.00047.2009. Endocannabinoids (ECs) act as retrograde messengers that enable postsynaptic cells to regulate the strength of their synaptic inputs. Here, by using physiological and histological techniques, we showed that, unlike in other parts of the brain, excitatory inputs are more sensitive than inhibitory inputs to EC signaling in the dorsal cochlear nucleus (DCN), an auditory brainstem nucleus. The principal cells of the DCN, fusiform cells, integrate acoustic signals through nonplastic synapses located in the deep layer with multimodal sensory signals carried by plastic parallel fibers in the molecular layer. Parallel fibers contact fusiform cells and inhibitory interneurons, the cartwheel cells, which in turn inhibit fusiform cells. Postsynaptic depolarization or pairing of postsynaptic potentials (PSPs) with action potentials (APs) induced EC-mediated modulation of excitatory inputs but did not affect inhibitory inputs. Quantitative electron microscopical studies showed that glutamatergic terminals express more cannabinoid 1 receptors (CB1Rs) than glycinergic terminals. Fusiform and cartwheel cells express diacylglycerol lipase ␣ and ␤ (DGL␣/␤), the two enzymes involved in the generation of the EC, 2-arachidonoyl-glycerol (2-AG). DGL␣ and DGL␤ are found in the spines of cartwheel but not fusiform cells indicating that the synthesis of ECs is more distant from parallel fiber synapses in fusiform than cartwheel cells. The differential localization and density of DGL␣/␤ and CB1Rs leads to cell-and input-specific EC signaling that favors activitydependent EC-mediated suppression at synapses between parallel fibers and cartwheel cell spines, thus leading to reduced feedforward inhibition in fusiform cells. We propose that EC signaling is a major modulator of the balance of excitation and inhibition in auditory circuits.

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“…Unilateral damage to the peripheral auditory system induces bilateral changes in the anatomical and physiological characteristics of cochlear nucleus neurons Suneja et al 1998;Muly et al 2002Muly et al , 2004Sumner et al 2005;Rubio 2006). This plasticity may be induced by ossicular chain disruption, cochlea and auditory nerve ablation, or intense sound exposure.…”

Section: Functional Considerationsmentioning

confidence: 99%

Commissural Neurons in the Rat Ventral Cochlear Nucleus

Doucet

Lenihan

May

2009

JARO

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Commissural neurons connect the cochlear nucleus complexes of both ears. Previous studies have suggested that the neurons may be separated into two anatomical subtypes on the basis of percent apposition (PA); that is, the percentage of the soma apposed by synaptic terminals. The present study combined tract tracing with synaptic immunolabeling to compare the soma area, relative number, and location of Type I (low PA) and Type II (high PA) commissural neurons in the ventral cochlear nucleus (VCN) of rats. Confocal microscopic analysis revealed that 261 of 377 (69%) commissural neurons have mediumsized somata with Type I axosomatic innervation. The commissural neurons also showed distinct topographical distributions. The majority of Type I neurons were located in the small cell cap of the VCN, which serves as a nexus for regulatory pathways within the auditory brainstem. Most Type II neurons were found in the magnocellular core. This anatomical dichotomy should broaden current views on the function of the commissural pathway that stress the fast inhibitory interactions generated by Type II neurons. The more prevalent Type I neurons may underlie slow regulatory influences that enhance binaural processing or the recovery of function after injury.

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Redistribution of synaptic AMPA receptors at glutamatergic synapses in the dorsal cochlear nucleus as an early response to cochlear ablation in rats

Cited by 46 publications

References 63 publications

The Planar Polarity Protein Scribble1 Is Essential for Neuronal Plasticity and Brain Function

The Planar Polarity Protein Scribble1 Is Essential for Neuronal Plasticity and Brain Function

Distinct Functional and Anatomical Architecture of the Endocannabinoid System in the Auditory Brainstem

Commissural Neurons in the Rat Ventral Cochlear Nucleus

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