Redox-active chemical chaperones exhibiting promiscuous binding promote oxidative protein folding under condensed sub-millimolar conditions

Suzuki, Koki; Nojiri, Ryoya; Matsusaki, Motonori; Mabuchi, Takuya; Kanemura, Shingo; Ishii, Kotone; Kumeta, Hiroyuki; Okumura, Masaki; Saio, Tomohide; Muraoka, Takahiro

doi:10.1039/d4sc02123a

Chem. Sci.

2024

DOI: 10.1039/d4sc02123a

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Redox-active chemical chaperones exhibiting promiscuous binding promote oxidative protein folding under condensed sub-millimolar conditions

Koki Suzuki,

Ryoya Nojiri,

Motonori Matsusaki

et al.

Abstract: A promiscuous protein binder with redox activity efficiently promotes oxidative protein folding under condensed sub-millimolar conditions.

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ER Oxidoreductin 1‐Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System

Mikami,

Nishizawa,

Iwata

et al. 2024

ChemBioChem

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Disulfide formation generally involves a two‐electron oxidation reaction between cysteine residues. Additionally, disulfide formation is an essential post‐translational modification for the structural maturation of proteins. This oxidative folding is precisely controlled by an electron relay network constructed by protein disulfide isomerase (PDI), with a CGHC sequence as the redox‐active site, and its family enzymes. Creating reagents that mimic the functions of these enzymes facilitates folding during chemical protein synthesis. In this study, we aimed to imitate a biological electron relay system using cyclic diselenide compounds as surrogates for endoplasmic reticulum oxidoreductin 1 (Ero1), which is responsible for the re‐oxidation of PDI. Oxidized PDI (PDIox) introduces disulfide bonds into substrate proteins, resulting in its conversion to reduced PDI (PDIred). The PDIred is then re‐oxidized to PDIox by a coexisting cyclic diselenide compound, thereby restoring the function of PDI as a disulfide‐forming agent. The produced diselenol state is readily oxidized to the original diselenide state with molecular oxygen, continuously sustaining the PDI catalytic cycle. This artificial electron relay system regulating enzymatic PDI function effectively promotes the oxidative folding of disulfide‐containing proteins, such as insulin—a hypoglycemic formulation—by enhancing both yield and reaction velocity.

show abstract

ER Oxidoreductin 1‐Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System

Mikami,

Nishizawa,

Iwata

et al. 2024

ChemBioChem

View full text Add to dashboard Cite

show abstract

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Redox-active chemical chaperones exhibiting promiscuous binding promote oxidative protein folding under condensed sub-millimolar conditions

Abstract: A promiscuous protein binder with redox activity efficiently promotes oxidative protein folding under condensed sub-millimolar conditions.

Cited by 1 publication

References 60 publications

ER Oxidoreductin 1‐Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System

ER Oxidoreductin 1‐Like Activity of Cyclic Diselenides Drives Protein Disulfide Isomerase in an Electron Relay System

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