Redox-active injectable gel using thermo-responsive nanoscale polyion complex flower micelle for noninvasive treatment of local inflammation

Pua, Min Ley; Yoshitomi, Toru; Chonpathompikunlert, Pennapa; Hirayama, Aki; Nagasaki, Yukio

doi:10.1016/j.jconrel.2013.10.009

Cited by 49 publications

(60 citation statements)

References 33 publications

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“…Because they show bioactivity in the body, the covalent linkage of nitroxide radicals to the polymer backbone is important to avoid inhalation of the nitroxide radicals. Thus far, we have developed polymeric micelles [12–21] and injectable hydrogels [22] possessing nitroxide radicals, 2,2,6,6-tetramethylpiperidine- N -oxyl (TEMPO), and demonstrated their in vitro and in vivo ROS-scavenging abilities. However, it is not clear whether nitroxide radicals scavenge ROS in the gaseous phase.…”

Section: Introductionmentioning

confidence: 99%

Development of nitroxide radicals–containing polymer for scavenging reactive oxygen species from cigarette smoke

Yoshitomi

Kuramochi

Vong

et al. 2014

Science and Technology of Advanced Materials

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We developed a nitroxide radicals–containing polymer (NRP), which is composed of poly(4-methylstyrene) possessing nitroxide radicals as a side chain via amine linkage, to scavenge reactive oxygen species (ROS) from cigarette smoke. In this study, the NRP was coated onto cigarette filters and its ROS-scavenging activity from streaming cigarette smoke was evaluated. The intensity of electron spin resonance signals of the NRP in the filter decreased after exposure to cigarette smoke, indicating consumption of nitroxide radicals. To evaluate the ROS-scavenging activity of the NRP-coated filter, the amount of peroxy radicals in an extract of cigarette smoke was measured using UV–visible spectrophotometry and 1,1-diphenyl-2-picrylhydrazyl (DPPH). The absorbance of DPPH at 517 nm decreased with exposure to cigarette smoke. When NRP-coated filters were used, the decrease in the absorbance of DPPH was prevented. In contrast, both poly[4-(cyclohexylamino)methylstyrene]- and poly(acrylic acid)-coated filters, which have no nitroxide radical, did not show any effect, indicating that the nitroxide radicals in the NRP scavenge the ROS in cigarette smoke. As a result, the extract of cigarette smoke passed through the NRP-coated filter has a lower cellular toxicity than smoke passed through poly[4-(cyclohexylamino)methylstyrene]- and poly(acrylic acid)-coated filters. Accordingly, NRP is a promising material for ROS scavenging from cigarette smoke.

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Section: Introductionmentioning

confidence: 99%

Development of nitroxide radicals–containing polymer for scavenging reactive oxygen species from cigarette smoke

Yoshitomi

Kuramochi

Vong

et al. 2014

Science and Technology of Advanced Materials

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“…The mice were fasted overnight but provided free access to water. The mice were divided into seven groups ( n = 6) and treated as follows: exenatide‐loaded RIG (exenatide: 14 μg, RIG: 72.5 mg/mL, 200 μL) every 7 days; exenatide‐loaded nRIG (indicating injectable gel without ROS scavenging effect, and which was prepared as previously reported …”

Section: Methodsmentioning

confidence: 99%

“…In addition, PMNT–PEG–PMNT possesses ROS‐scavenging moieties as side chains. RIG can therefore suppress adverse events such as ROS‐induced oxidative stress …”

Section: Introductionmentioning

confidence: 99%

Redox‐active injectable gel using polyion complex to achieve sustained release of exenatide and enhance therapeutic efficacy for the treatment of type 2 diabetes

Shiro

Sakaue

Nagasaki

2019

J Biomedical Materials Res

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To provide sustained release of exenatide and enhance therapeutic efficacy for the treatment of type 2 diabetes compared to the existing products for exenatide, we developed an exenatide‐loaded, redox‐active, injectable gel (Exe@RIG). This injectable gel is formed by a polyion complex (PIC) comprising three components, (1) cationic polyamine–poly(ethylene glycol)–polyamine triblock copolymer possessing reactive oxygen species (ROS)‐scavenging moieties as side chains, (2) anionic poly(acrylic acid), and (3) exenatide. The mixture formed exenatide‐loaded PIC flower micelles at room temperature, which immediately converted to a gel under physiological conditions. Owing to electrostatic interactions between exenatide and the PIC gel network, RIG was able to provide sustained release of exenatide without a significant initial burst. Subcutaneous injection of Exe@RIG once a week prevented the increase in glucose concentration significantly in db/db mice compared to those in control groups. In addition, Exe@RIG suppressed the degeneration of pancreatic islets, which is reported to be caused by increased ROS. Our result indicates that Exe@RIG has the potential to provide a long acting exenatide as well as enhanced efficacy in the treatment of type 2 diabetes compared to the existing products. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1107–1113, 2019.

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“…In a carrageenan-induced arthritis model, RIG inhibits neutrophil infi ltration and cytokine production, and showed treatment effect on the local infl ammation. [ 63 ] In another study by the same group, RIG was also used as an antiadhesion agent. [ 64 ] RIG was placed to the abdominal wall of mice with talcinduced severe infl ammation and adhesion in the peritoneal cavity.…”

Section: Materials Protecting Cells From Ros Damagementioning

confidence: 99%

Reactive Oxygen Species (ROS) Responsive Polymers for Biomedical Applications

Xiao

et al. 2016

Macromolecular Bioscience

304

202

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Reactive oxygen species (ROS) play important roles in cell signaling pathways, while increased production of ROS may disrupt cellular homeostasis, giving rise to oxidative stress and a series of diseases. Utilizing these cell‐generated species as triggers for selective tuning polymer structures and properties represents a promising methodology for disease diagnosis and treatment. Recently, significant progress has been made in fabricating biomaterials including nanoparticles and macroscopic networks to interact with this dynamic physiological condition. These ROS‐responsive platforms have shown potential in a range of biomedical applications, such as cancer targeted drug delivery systems, cell therapy platforms for inflammation related disease, and so on.

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Redox-active injectable gel using thermo-responsive nanoscale polyion complex flower micelle for noninvasive treatment of local inflammation

Cited by 49 publications

References 33 publications

Development of nitroxide radicals–containing polymer for scavenging reactive oxygen species from cigarette smoke

Development of nitroxide radicals–containing polymer for scavenging reactive oxygen species from cigarette smoke

Redox‐active injectable gel using polyion complex to achieve sustained release of exenatide and enhance therapeutic efficacy for the treatment of type 2 diabetes

Reactive Oxygen Species (ROS) Responsive Polymers for Biomedical Applications

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