Redox and global interconnected proteome changes in mice exposed to complex environmental hazards surrounding Doñana National Park

Meta‐omic evaluation of bacterial microbial community structure and activity for the environmental assessment of soils: overcoming protein extraction pitfalls

“…Anyhow, the different uptake or extrusion mechanisms and the broad and diverse range of substrate exchanged allow bacteria to quickly adapt to and colonize changing environments (Jeckelmann and Erni, 2020) Furthermore, several proteins that showed significant increases in the two polluted sites, AJO and MAT, are directly related to the defence against environmental stress (Fig. 6 and Supplementary Table 3), many of which have previously been shown to respond to environmental pollution, including dioxygenase (Sharma et al ., 2019), glyceraldehyde‐3‐phosphate dehydrogenase (Reyes‐Hernández et al ., 2009; Michán et al ., 2019), fructose‐biphosphate aldolase (Fernández‐Cisnal et al ., 2014, 2017; Michán et al ., 2019), cysteine synthase (Ding et al ., 2021), isocitrate dehydrogenase (Fernández‐Cisnal et al ., 2017), enolase (Puglisi et al ., 2010; Michán et al ., 2019), ATP‐dependent zinc metalloprotease FTSH (Tang et al ., 2016), catalase‐peroxidase (Puglisi et al ., 2010; Michán et al ., 2019), chlorite dismutase (Hofbauer et al ., 2014), thiosulfate sulfurtransferase (Michán et al ., 2019), peptide methionine sulfoxide reductase MsrA (Weissbach et al ., 2002), superoxide dismutase (Puglisi et al ., 2010; Fernández‐Cisnal et al ., 2014), FMN‐dependent oxidoreductase (Ellis, 2010; Puglisi et al ., 2010), aldehyde dehydrogenase (Puglisi et al ., 2010; Abril et al ., 2015), and so on. All these proteins contribute to detoxification processes, to defence against oxidative stress or repair of cell damage after exposure to xenobiotics.…”

“…Coincident global responses were obtained in the soil microbiome with those that showed higher organisms (e.g. Mus spretus mice) in the same area studied as determined by label‐free quantitative proteomics analysis (Michán et al ., 2019). Matching responses include (i) Increased synthesis of protective antioxidant enzymes to fight against the oxidative stress generated by pollutants (Cluster III).…”

“…LDP, located in the heart of Doñana, is a highly protected area that has usually been considered as a pristine ecosystem although several studies have nuanced this assumption (García‐Sevillano et al ., 2012; Michán et al ., 2019). However, DNP is surrounded by many anthropogenic activities that threaten its ecological balance and environmental health (Abril et al ., 2011; García‐Sevillano et al ., 2012; Fernández‐Cisnal et al ., 2014; García‐Sevillano et al ., 2014; Abril et al ., 2015; Fernández‐Cisnal et al ., 2017; Michán et al ., 2019). We studied two areas that border the National Park: in the northwest, AJO is close to extensive areas covered with strawberry greenhouses and vineyards and is also affected by wastes from several urban enclaves; in the east, MAT is affected by paddies and suffers the input of pesticides and metals used as algaecides during the rice‐growing process (García‐Sevillano et al ., 2012; García‐Sevillano et al ., 2014; Abril et al ., 2015).…”

“…1). LDP, located in the heart of Doñana, is a highly protected area that has usually been considered as a pristine ecosystem although several studies have nuanced this assumption (García‐Sevillano et al ., 2012; Michán et al ., 2019). However, DNP is surrounded by many anthropogenic activities that threaten its ecological balance and environmental health (Abril et al ., 2011; García‐Sevillano et al ., 2012; Fernández‐Cisnal et al ., 2014; García‐Sevillano et al ., 2014; Abril et al ., 2015; Fernández‐Cisnal et al ., 2017; Michán et al ., 2019).…”

“…However, despite its great ecological value, DNP is extremely sensitive. Doñana is surrounded by areas exposed to numerous threats of pollution due to agricultural, mining and industrial undertakings (Abril et al ., 2014; Fernández‐Cisnal et al ., 2014; García‐Sevillano et al ., 2014; Abril et al ., 2015; Ruiz‐Laguna et al ., 2016; Michán et al ., 2019). There is an urgent need to assess and identify early responses to pollution to prevent excessive or even irreversible damage to ecosystems.…”