Redox Brake Regulator RedB and FnrL Function as Yin-Yang Regulators of Anaerobic-Aerobic Metabolism in Rhodobacter capsulatus

Ke, Nijia; Kumka, Joseph E.; Fang, Mingxu; Weaver, Brian R; Burstyn, Judith N.; Bauer, Carl E.

doi:10.1128/spectrum.02354-22

Cited by 3 publications

(4 citation statements)

References 50 publications

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“…Finally, in a companion article, we show that many genes regulated by RedB significantly overlap genes that constitute members of the FnrL regulon (the Rhodobacter ortholog of FNR) ( 28 ). Thus, RedB appears to have a major role in functioning as a damper to counter the energy-producing role of FnrL ( 28 ).…”

Section: Resultsmentioning

confidence: 93%

“…Regarding direct control (Table S2), RedB directly activates the expression of dorS , which encodes the DMSO-TMAO sensor hybrid histidine kinase ( 27 ), as there are two ChIP-seq peaks upstream of dorS . In the presence of trimethylamine N-oxide (TMAO), DorS phosphorylates the transcriptional regulator DorR, which then upregulates the expression of torA , which codes for the terminal electron acceptor TMAO reductase ( 28 , 29 ). Interestingly, the RNA-seq results also show that RedB downregulates the expression of torA 1.33-fold instead of upregulating it.…”

Section: Resultsmentioning

confidence: 99%

“…For validation of the expression levels observed by RNA-seq, we performed qRT-PCR using primers designed to amplify select RT transcripts, as previously described by Kumka and Bauer ( 19 ). As shown in the companion article by Ke et al ( 28 ), there are more highly significant divergent changes in gene expression between the Δ fnrL and Δ redB deletion strains than between Δ redB and wild-type cells. Thus, comparative qRT-PCR validation analysis of the Δ redB and Δ fnrL strains was undertaken to validate the observed divergent changes in transcript levels.…”

Section: Methodsmentioning

confidence: 91%

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RedB, a Member of the CRP/FNR Family, Functions as a Transcriptional Redox Brake

Kumka

Fang

et al. 2022

Microbiol Spectr

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 91%

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RedB, a Member of the CRP/FNR Family, Functions as a Transcriptional Redox Brake

Kumka

Fang

et al. 2022

Microbiol Spectr

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“…Certainly, ArcR is not an exception. Other Crp/Fnr family members can also repress the expression of functional genes such as Fnr, RedB, DnrD, and DnrF [ 25 , 42 , 43 ], as the NCTC8325 strain is known to be defective in sigma factor SigB at the stationary phase [ 44 ]. Meanwhile, to exclude strain-specific differences, the virulence- and biofilm-associated experiments were repeated with the arcR mutant under USA300 background.…”

Section: Discussionmentioning

confidence: 99%

Roles of the Crp/Fnr Family Regulator ArcR in the Hemolysis and Biofilm of Staphylococcus aureus

Fan

et al. 2023

Microorganisms

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Staphylococcus aureus is an opportunistic human pathogen that is often involved in severe infections such as pneumonia and sepsis in which bacterial virulence factors play a key role. Infections caused by S. aureus are often difficult to eradicate, particularly when they are associated with biofilm. The physiological roles of the Crp/Fnr family regulator ArcR are elusive in S. aureus. In this study, it was found that the deletion of arcR increased the hemolytic ability and biofilm formation in S. aureus. Differential gene expression analysis by RNA-seq and real-time quantitative reverse transcription PCR showed that genes associated with hemolytic ability (hla and hlb) and biofilm formation (icaA, icaB, icaC and icaD) were significantly upregulated compared with those in the wild-type strain. The results revealed that ArcR regulated the expression of the hla and ica operon by binding to their promoter regions, respectively. This study provided new insights into the functional importance of ArcR in regulating the virulence and biofilm of S. aureus.

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The cAMP receptor protein from Gardnerella vaginalis is not regulated by ligands

Dong,

Zhang,

Zhang

et al. 2024

Commun Biol

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Overgrowth of Gardnerella vaginalis causes an imbalance in vaginal microecology. The pathogenicity of G. vaginalis is directly regulated by the cAMP receptor protein (CRP). In this study, we resolve the crystal structure of CRP Gv at a resolution of 2.22 Å and find some significant differences from homologous proteins. The first 23 amino acids of CRP Gv are inserted into the ligand binding pocket, creating a strong steric barrier to ligand entry that has not been seen previously in its homologues. In the absence of ligands, the two α helices used by CRP Gv to bind oligonucleotide chains are exposed and can specifically bind TGTGA-N6-TCACA sequences. cAMP and other ligands of CRP homologs are not cofactors of CRP Gv . There is no coding gene of the adenylate cyclase, and cAMP could not be identified in G. vaginalis by liquid chromatography tandem mass spectrometry. We speculate that CRP Gv may achieve fine regulation through a conformational transformation different from that of its homologous proteins, and this conformational transformation is no longer dependent on small molecules, but may be aided by accessory proteins. CRP Gv is the first discovered CRP that is not ligand-regulated, and its active conformation provides a structural basis for drug screening.

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Redox Brake Regulator RedB and FnrL Function as Yin-Yang Regulators of Anaerobic-Aerobic Metabolism in Rhodobacter capsulatus

Cited by 3 publications

References 50 publications

RedB, a Member of the CRP/FNR Family, Functions as a Transcriptional Redox Brake

RedB, a Member of the CRP/FNR Family, Functions as a Transcriptional Redox Brake

Roles of the Crp/Fnr Family Regulator ArcR in the Hemolysis and Biofilm of Staphylococcus aureus

The cAMP receptor protein from Gardnerella vaginalis is not regulated by ligands

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