Redox Control in Human Disease with a Special Emphasis on the Peroxiredoxin‐Based Antioxidant System

Szabó, Katalin; Gutowski, Nicholas J.; Holley, Janet E.; Littlechild, Jennifer A.; Winyard, Paul G.

doi:10.1002/9783527627585.ch18

Cited by 5 publications

(3 citation statements)

References 129 publications

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“…Therefore, as a next step, we aimed to analyse the distribution of the thiol containing redox protein, peroxiredoxin 1, in the samples. Prdx 1 has a known role in inflammation and autoimmune processes [17], and is also known to be secreted into the blood plasma both as a soluble protein and as a protein associated with exosomes [18].…”

Section: Exofacial Peroxiredoxin 1 (Prdx 1) Is Present On Monocytes Prdx 1 Positive Evs Are More Numerous In Ra Blood Plasma Than In Healmentioning

confidence: 99%

Monocyte activation drives preservation of membrane thiols by promoting release of oxidised membrane moieties via extracellular vesicles

Szabó-Taylor

Tóth

Balogh

et al. 2017

Free Radical Biology and Medicine

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The redox state of cellular exofacial molecules is reflected by the amount of available thiols. Furthermore, surface thiols can be considered as indicators of immune cell activation. One group of thiol containing proteins, peroxiredoxins, in particular, have been associated with inflammation. In this study, we assessed surface thiols of the U937 and Thp1 monocyte cell lines and primary monocytes in vitro upon inflammatory stimulation by irreversibly labelling the cells with a fluorescent derivative of maleimide. We also investigated exofacial thiols on circulating blood mononuclear cells in patients with rheumatoid arthritis and healthy controls. When analysing extracellular vesicles, we combined thiol labelling with the use of antibodies to specific CD markers to exclude extracellular vesicle mimicking signals from thiol containing protein aggregates. Furthermore, differential detergent lysis was applied to confirm the vesicular nature of the detected extracellular events in blood plasma. We found an increase in exofacial thiols on monocytes upon in vitro stimulation by LPS or TNF, both in primary monocytes and monocytic cell lines (p<0.0005). At the same time, newly released extracellular vesicles showed a decrease in their exofacial thiols compared with those from unstimulated cells (p<0.05). We also found a significant elevation of surface thiols on circulating monocytes in rheumatoid arthritis patients (p<0.05) and newly released extracellular vesicles of isolated CD14 cells from rheumatoid arthritis patients had decreased thiol levels compared with healthy subjects (p<0.01). Exofacial peroxiredoxin 1 was demonstrated on the surface of primary and cultured monocytes, and the number of peroxiredoxin 1 positive extracellular vesicles was increased in rheumatoid arthritis blood plasma (p<0.05). Furthermore, an overoxidised form of peroxiredoxin was detected in extracellular vesicle-enriched preparations from blood plasma. Our data show that cell surface thiols play a protective role and reflect oxidative stress resistance state in activated immune cells. Furthermore, they support a role of extracellular vesicles in the redox regulation of human monocytes, possibly representing an antioxidant mechanism.

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Section: Exofacial Peroxiredoxin 1 (Prdx 1) Is Present On Monocytes Prdx 1 Positive Evs Are More Numerous In Ra Blood Plasma Than In Healmentioning

confidence: 99%

Monocyte activation drives preservation of membrane thiols by promoting release of oxidised membrane moieties via extracellular vesicles

Szabó-Taylor

Tóth

Balogh

et al. 2017

Free Radical Biology and Medicine

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“…Autoantigens and immunoglobulins are not the solely soluble proteins that associate with EVs during the development of autoimmune diseases (Buzas et al., 2014 ; Cloutier et al., 2013 ; Fortin et al., 2016 ; Nielsen et al., 2012 ). Peroxiredoxin 1 (Prdx 1) has a role in inflammation and autoimmune processes (Szabó et al., 2009 ). It is present in the blood plasma both as a soluble protein and associated with EVs (Mullen et al., 2015 ).…”

Section: Role Of the Soluble Proteins Acquired By Evs In Biologic Pro...mentioning

confidence: 99%

The functional role of soluble proteins acquired by extracellular vesicles

Ramos

Sebinelli

Ciancaglini

et al. 2022

J of Extracellular Bio

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Extracellular vesicles (EVs) are lipid bilayer‐enclosed nanosized particles released by all cell types during physiological as well as pathophysiological processes to carry out diverse biological functions, including acting as sources of cellular dumping, signalosomes and mineralisation nanoreactors. The ability of EVs to perform specific biological functions is due to their biochemical machinery. Among the components of the EVs’ biochemical machinery, surface proteins are of critical functional significance as they mediate the interactions of EVs with components of the extracellular milieu, the extracellular matrix and neighbouring cells. Surface proteins are thought to be native, that is, pre‐assembled on the EVs’ surface by the parent cells before the vesicles are released. However, numerous pieces of evidence have suggested that soluble proteins are acquired by the EVs’ surface from the extracellular milieu and further modulate the biological functions of EVs during innate and adaptive immune responses, autoimmune disorders, complement activation, coagulation, viral infection and biomineralisation. Herein, we will describe the methods currently used to identify the EVs’ surface proteins and discuss recent knowledge on the functional relevance of the soluble proteins acquired by EVs.

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“…Nesse sentido, apesar das funções celulares exercidas pelos HPx em baixos níveis celulares, seu excesso pode desequilibrar a homeostase celular causando um fenômeno conhecido como estresse oxidativo. Para manter o equilíbrio necessário e regular as atividades dos HPx, as células utilizam moléculas de baixo peso molecular como a glutationa e o ácido ascórbico, dentre outras, bem como de enzimas antioxidantes como as glutationa peroxidases (GPx), catalases (Cat) e peroxirredoxinas (Prx) (DAY et al, 2012;KANG, S. W. et al, 2005;MONTEIRO et al, 2007;POWIS;KIRKPATRICK, 2007;SZABÓ et al, 2009;THOMSEN;MILES, 1998) as quais serão discutidas em detalhe posteriormente. HARRIS, 2003;LÓPEZ-LÁZARO, 2007;SEIFRIED et al, 2007).…”

Section: Hidroperóxidos: Tipos E Relevância Em Processos Biológicosunclassified

Estudos estruturais e funcionais de Tsa1 de <i>Saccharomyces cerevisiae</i>: um modelo biológico para o estudo de inibidores de crescimento celular para leucemia linfoide aguda

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Redox Control in Human Disease with a Special Emphasis on the Peroxiredoxin‐Based Antioxidant System

Cited by 5 publications

References 129 publications

Monocyte activation drives preservation of membrane thiols by promoting release of oxidised membrane moieties via extracellular vesicles

Monocyte activation drives preservation of membrane thiols by promoting release of oxidised membrane moieties via extracellular vesicles

The functional role of soluble proteins acquired by extracellular vesicles

Estudos estruturais e funcionais de Tsa1 de <i>Saccharomyces cerevisiae</i>: um modelo biológico para o estudo de inibidores de crescimento celular para leucemia linfoide aguda

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