Redox potential change by the cystine importer affected on enzymatic antioxidant protection in Deinococcus geothermalis

Choo, Kyung‐sil; Kim, Minwook; Nansa, Sama Abdi; Bae, Min K.; Lee, Chanjae; Lee, Sung–Jae

doi:10.1007/s10482-020-01388-4

Cited by 11 publications

(22 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the functional role of the putative LysR family regulator Dgeo_2840, we constructed target gene disrupted mutant strain using homologous recombination procedure, and accurately detected using size enlargement of the target gene amplification and DNA sequencing ( Figures 1A,B ). In complementation assay, the shuttle vector pRADgro was used ( Choo et al, 2020 ) and the recombinant DNA including intact dgeo _2840 gene was transformed into wild-type and Δ dgeo _2840 mutant strain. The empty vector and recombinant DNA were well-transformed into wild-type strain and selected on chloramphenicol contained medium ( Supplementary Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…The resulting transformed strain was named Δ dgeo_ 2840 mutant, and selected on TGY agar containing 8 μl/ml kanamycin for 2 days incubation at 48°C. The complementation assay of dgeo _2840 gene was performed following previous study ( Choo et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous work, quantitative RT-PCR analysis was performed on four LysR genes, including OxyR for oxidation and reduction states. OxyR was highly up-regulated on hydrogen peroxide treatment or the oxidized condition through cystine-importer disruption (Kim et al, 2017;Choo et al, 2020). Other LysR-member protein Dgeo_2840 was also induced in both early and late exponential growth phase and oxidative stress conditions (data not shown).…”

Section: Introductionmentioning

confidence: 91%

“…The final recombinant plasmid pKRL2840 was purified from E. coli, and transformed into D. geothermalis competent cell using a CaCl 2 -dependent technique described previously (Kim et al, 2017). The resulting transformed strain was named dgeo_2840 mutant, and selected on TGY agar containing 8 µl/ml kanamycin for 2 days incubation at 48 • C. The complementation assay of dgeo_2840 gene was performed following previous study (Choo et al, 2020).…”

Section: Strains Culture Conditions and Construction Of The Mutantmentioning

confidence: 99%

See 3 more Smart Citations

Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis

2020

Self Cite

View full text Add to dashboard Cite

Radiation-resistant bacterium Deinococcus geothermalis has a total of 73 insertion sequences (ISs) in genomes, and some of them are actively transposed to other loci with replicative mode due to oxidative stress of hydrogen peroxide treatment. Here, we detected two transposition events in wild-type (WT) strain and LysR family member gene disrupted strain (dgeo_2840). Similar to our previous report (Lee et al., 2019), phytoene desaturase (dgeo_0524), a key enzyme of carotenoid biosynthesis, was disrupted by the integration of IS element, thereby detected a single phenotypically non-pigmented colony in each WT and dgeo_2840 strain. Two separate types of IS element have been integrated into non-pigmented clones: ISDge11 for WT and ISDge6 for dgeo_2840 strain. Surprisingly, dgeo_2840 mutant strain revealed higher resistance to oxidative stress than WT strain at late exponential growth phase. From the qRT-PCR analysis, OxyR (dgeo_1888) was highly up-regulated to 30-fold by oxidative stress through hydrogen peroxide treatment in both WT and dgeo_2840 mutant strains. However, the oxidative stress response enzyme, catalase or superoxide dismutase, was not significantly induced by overexpressed OxyR. Thus, a putative LysR family regulator Dgeo_2840 controlled the expression of ISDge6 type transposase and the induction of OxyR under oxidative condition. There is LysR family DNA-binding protein dependent active transposition of specific type IS and the up-regulated OxyR has not positively controlled ROS scavenger enzymes in D. geothermalis.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Strains Culture Conditions and Construction Of The Mutantmentioning

confidence: 99%

See 2 more Smart Citations

Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…WordCloud analysis of our owns four published papers [25,55,61,62] by the WordCloud generator 40 quantity of MonkeyLearn.…”

Section: Figurementioning

confidence: 99%

An Antioxidant Defense System in Radiation-Resistant Bacterium Deinococcus geothermalis against Oxidative Stress

Lee¹,

Bae²,

Lee³

2021

Antioxidants - Benefits, Sources, Mechanisms of Action

Self Cite

View full text Add to dashboard Cite

A radiation-resistant bacterium, Deinococcus geothermalis has various stress response mechanisms, including antioxidation. Features that maintain vitality at high radiation doses include the following: enzymatic scavengers of ROS such as catalase, SOD, and peroxidase; strain-specific DNA repair systems such as Deinococcal unique proteins; non-enzymatic responses such as manganese complexes, carotenoids, and DNA-binding proteins. This chapter summarizes the primary response mechanism by redox balance centered on the cystine transporter. It also reviews action characteristics of DNA-binding protein Dps and a putative LysR family protein, and effects on loss of function of the carotenoid biosynthesis genes by transposition of insertion sequences. Environmental adaptation and molecular evolution of radiation-resistant bacterium are also considered to explain the potentials of molecular behavior induced by oxidative stress.

show abstract

Transposition of insertion sequences by dielectric barrier discharge plasma and gamma irradiation in the radiation-resistant bacterium Deinococcus geothermalis

Shin

Lee

et al. 2022

Journal of Microbiological Methods

View full text Add to dashboard Cite

Redox potential change by the cystine importer affected on enzymatic antioxidant protection in Deinococcus geothermalis

Cited by 11 publications

References 40 publications

Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis

Active Transposition of Insertion Sequences by Oxidative Stress in Deinococcus geothermalis

An Antioxidant Defense System in Radiation-Resistant Bacterium Deinococcus geothermalis against Oxidative Stress

Transposition of insertion sequences by dielectric barrier discharge plasma and gamma irradiation in the radiation-resistant bacterium Deinococcus geothermalis

Contact Info

Product

Resources

About