Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila

Choi, Tae-Yeong; Park, So Young; Kang, Hyen Sam; Cheong, JaeHun; Kim, Han‐Do; Lee, Bok Luel; Hirose, Fumiko; Yamaguchi, Masamitsu; Yoo, Mi‐Ae

doi:10.1042/bj20031601

Cited by 13 publications

(6 citation statements)

References 33 publications

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“…In a recent study, we found that the DNA binding activity of DREF is directly modulated by elevated ROS through the effect of ROS on two cysteine residues located in the DNAbinding domain of DREF (36). One prediction of a functional relationship between DREF and catalase expression is that reducing catalase expression might suppress a phenotype associated with ectopic DREF over-expression.…”

Section: Catalase Amorphic Cat N1 Mutation Suppresses the Dref Over-expression-induced Rough Eye Phenotypementioning

confidence: 96%

Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system

Park

Kim²,

Yang³

et al. 2004

Nucleic Acids Research

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Reactive oxygen species (ROS) cause oxidative stress and aging. The catalase gene is a key component of the cellular antioxidant defense network. However, the molecular mechanisms that regulate catalase gene expression are poorly understood. In this study, we have identified a DNA replication-related element (DRE; 5'-TATCGATA) in the 5'-flanking region of the Drosophila catalase gene. Gel mobility shift assays revealed that a previously identified factor called DREF (DRE- binding factor) binds to the DRE sequence in the Drosophila catalase gene. We used site-directed mutagenesis and in vitro transient transfection assays to establish that expression of the catalase gene is regulated by DREF through the DRE site. To explore the role of DRE/DREF in vivo, we established transgenic flies carrying a catalase-lacZ fusion gene with or without mutation in the DRE. The beta-galactosidase expression patterns of these reporter transgenic lines demonstrated that the catalase gene is upregulated by DREF through the DRE sequence. In addition, we observed suppression of the ectopic DREF-induced rough eye phenotype by a catalase amorphic Cat(n1) allele, indicating that DREF activity is modulated by the intracellular redox state. These results indicate that the DRE/DREF system is a key regulator of catalase gene expression and provide evidence of cross-talk between the DRE/DREF system and the antioxidant defense system.

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Section: Catalase Amorphic Cat N1 Mutation Suppresses the Dref Over-expression-induced Rough Eye Phenotypementioning

confidence: 96%

Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system

Park

Kim²,

Yang³

et al. 2004

Nucleic Acids Research

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“…Finally, the endogenous production of reactive oxygen species (ROS) as by-products of cellular metabolism can affect the REDOX status of replication factors and alter the initiation or progression of replication [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: The Multiple Causes Of the Replication Stressmentioning

confidence: 99%

Replication Stress in Mammalian Cells and Its Consequences for Mitosis

Gelot

Magdalou

Lopez

2015

Genes

112

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The faithful transmission of genetic information to daughter cells is central to maintaining genomic stability and relies on the accurate and complete duplication of genetic material during each cell cycle. However, the genome is routinely exposed to endogenous and exogenous stresses that can impede the progression of replication. Such replication stress can be an early cause of cancer or initiate senescence. Replication stress, which primarily occurs during S phase, results in consequences during mitosis, jeopardizing chromosome segregation and, in turn, genomic stability. The traces of replication stress can be detected in the daughter cells during G1 phase. Alterations in mitosis occur in two types: 1) local alterations that correspond to breaks, rearrangements, intertwined DNA molecules or non-separated sister chromatids that are confined to the region of the replication dysfunction; 2) genome-wide chromosome segregation resulting from centrosome amplification (although centrosomes do not contain DNA), which amplifies the local replication stress to the entire genome. Here, we discuss the endogenous causes of replication perturbations, the mechanisms of replication fork restart and the consequences for mitosis, chromosome segregation and genomic stability.

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“…Transfection of various DNA mixtures into Kc cells was performed using dimethyldioctadecyl ammonium bromide (DDAB) and the cells were harvested 48 h thereafter. The luciferase assay was carried out by means of a Luciferase Assay System (Promega), as previously described (Choi et al 2004). Luciferase activity was normalized with β-galactosidase activity to correct for transfection efficiency.…”

Section: Cell Culture and Dna Transfectionmentioning

confidence: 99%

“…DREF also modulates the transcription of the Drosophila mitochondrial transcription factor A (D-mtTFA) gene (Takata et al 2001), and the accessory subunit gene of Drosophila mitochondrial DNA polymerase . The DNA binding activity of DREF is regulated directly by elevated levels of ROS (Choi et al 2004), and this activity is modulated via the intracellular redox state . It was reported that ectopic DREF expression in Drosophila eye and wing imaginal disc cells induces both apoptosis and aberrant morphogenesis Yoshida et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system

et al. 2007

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Adenine nucleotide translocase (ANT) is a crucial component in the maintenance of cellular energy homeostasis, as well as in the formation of the mitochondrial permeability transition pores. However, the molecular mechanisms regulating the expression of the ANT gene are poorly understood. In this study, we have identified three DNA replication-related elements (DRE; 5′ ′ ′ ′-TATCGATA) in the 5′ ′ ′ ′-flanking region of the Drosophila ANT (dANT ) gene. Gel-mobility shift analyses revealed that all three of the DREs were recognized by the DRE-binding factor (DREF). The site-directed mutagenesis of these DRE sites induces a considerable reduction in the activity of the dANT gene promoter in vitro. Analyses with transgenic flies harboring a dANT-lacZ fusion gene bearing the wild-type or mutant DRE sites showed that the DRE sites were required for the expression of dANT in vivo. We determined that the over-expression or knockdown of DREF exerts a regulatory effect on the activity of the dANT promoter. In addition, we observed the collapse of mitochondrial membrane potential in the eye imaginal discs in which DREF was overexpressed. These results show that DRE/DREF is a crucial regulator of dANT gene expression, and also suggest the possibility that cross-talk may occur between the DRE/DREF system and mitochondrial functioning.

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Redox regulation of DNA binding activity of DREF (DNA replication-related element binding factor) in Drosophila

Cited by 13 publications

References 33 publications

Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system

Transcriptional regulation of the Drosophila catalase gene by the DRE/DREF system

Replication Stress in Mammalian Cells and Its Consequences for Mitosis

Transcriptional regulation of the Drosophila ANT gene by the DRE/DREF system

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