Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues

Tichá, Tereza; Lochman, Jan; Činčalová, Lucie; Luhová, Lenka; Petřivalský, Marek

doi:10.1016/j.bbrc.2017.10.090

Cited by 36 publications

(19 citation statements)

References 33 publications

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“…Our data underscore the complexity of redox regulation in the biochemically intricate environment of the plant cell. They add to studies implicating changes of GSNOR activities in response to stress conditions such as MV, exogenous H 2 O 2 , nitrogen stress, and hypoxia, conditions in which redox‐based modification of the protein has previously been described (Chen et al, ; Frungillo et al, ; Kovacs et al, ; Tichá et al, ; Zhan et al, ). Although we note in previous reports that GSNOR1 activity may be inhibited by H 2 O 2 in vitro (Kovacs et al, ; Tichá et al, ), such effects may be counteracted in cat2 by up‐regulation of the amount of GSNOR1 protein (Figure ) or simply not operative because H 2 O 2 accumulation inside the cell is restricted to localized subcellular areas, transient in nature, and unlikely to greatly exceed values of about 10 μM (Han et al, ; Mhamdi et al, ; Rahantaniaina et al, ; Tuzet et al, ; Xu, Guerra, Lee, & Vierling, ).…”

Section: Discussionmentioning

confidence: 93%

“…Recent studies revealed that NO represses GSNOR activity through direct posttranslational modification of Cys residues (Frungillo et al, ; Guerra et al, ; Tichá et al, ), prompting us to test if the inhibition of GSNOR activity in cat2 pad2 was mediated by S ‐nitrosation of GSNOR1. For this, an NO fluorescent assay and the BST were employed.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, deregulation of GSNOR may impact glutathione‐mediated protein denitrosation. Intriguingly, GSNOR activity and stability are themselves modulated via S ‐nitrosation of multiple conserved Cys sites (Frungillo et al, ; Guerra, Ballard, Truebridge, & Vierling, ; Tichá, Lochman, Činčalová, Luhová, & Petřivalský, ; Zhan et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂

Zhang

Chen

et al. 2020

Plant Cell & Environment

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Photorespiratory hydrogen peroxide (H2O2) plays key roles in pathogenesis responses by triggering the salicylic acid (SA) pathway in Arabidopsis. However, factors linking intracellular H2O2 to activation of the SA pathway remain elusive. In this work, the catalase‐deficient Arabidopsis mutant, cat2, was exploited to elucidate the impact of S‐nitrosoglutathione reductase 1 (GSNOR1) on H2O2‐dependent signalling pathways. Introducing the gsnor1‐3 mutation into the cat2 background increased S‐nitrosothiol levels and abolished cat2‐triggered cell death, SA accumulation, and associated gene expression but had little additional effect on the major components of the ascorbate‐glutathione system or glycolate oxidase activities. Differential transcriptome profiles between gsnor1‐3 and cat2 gsnor1‐3 together with damped ROS‐triggered gene expression in cat2 gsnor1‐3 further indicated that GSNOR1 acts to mediate the SA pathway downstream of H2O2. Up‐regulation of GSNOR activity was compromised in cat2 cad2 and cat2 pad2 mutants in which glutathione accumulation was genetically prevented. Experiments with purified recombinant GSNOR revealed that the enzyme is posttranslationally regulated by direct denitrosation in a glutathione‐dependent manner. Together, our findings identify GSNOR1‐controlled nitrosation as a key factor in activation of the SA pathway by H2O2 and reveal that glutathione is required to maintain this biological function.

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Section: Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂

Zhang

Chen

et al. 2020

Plant Cell & Environment

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“…The resulting GSSG is then reduced again to GSH by the NADPH‐dependent reaction catalyzed by GR (Fatma et al ). GSNOR participates in regulation of ROS and generation of reactive nitrogen species (RNS) activation within the antioxidant system, the cellular redox status, induction of stress‐responsive genes, and stress‐induced death of entire plants (Tichá et al , Cheng et al ). Alterations in the GSH pools could have important implications in cellular redox status with impact on various cell signaling pathways (Vivancos et al ).…”

Section: Discussionmentioning

confidence: 99%

Treatment of nitric oxide supplemented with nitrogen and sulfur regulates photosynthetic performance and stomatal behavior in mustard under salt stress

Jahan

Alajmi

Rehman

et al. 2020

Physiologia Plantarum

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Nitric oxide (NO) is a hormone that connects numerous reactions in plant cells under normal and environmental stress conditions. The application of 100 μM NO as sodium nitroprusside (SNP; NO donor) applied individually or in combination with N or S in different combinations (i.e. 100 mg N or S kg −1 soil applied at the time of sowing [100 N + 100S] 0d or with split, 50 mg N or S kg −1 soil at the time of sowing and similar dose at 20 d after sowing [50 N + 50S] 0d + [50 N + 50S] 20d ) was tested to alleviate salt stress in mustard (Brassica juncea L.). Application of 100 μM NO plus split application of N and S more significantly promoted stomatal behavior, photosynthetic and growth performance in the absence of salt stress and maximally alleviated effects of salt stress through increased N-and S-use efficiency, proline and antioxidant system. The combined application of N and S at the time of sowing was lesser effective in promoting photosynthesis and growth under salt or no salt stress conditions in presence or absence of NO. The study suggests that salt stress effects on the photosynthetic performance are mitigated more efficiently when NO was applied together with the split application of N and S given at two stages, and the photosynthetic activity was promoted under salt stress through increased N and S assimilation and antioxidant system. This strategy may be adopted in agricultural system for overcoming salt stress effects on performance of mustard.

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“…GSNO is metabolized to oxidized glutathione disulfide and ammonia by cysteine‐rich GSNO reductases (GSNORs), which regulate development and defense (Kwon et al, ; Leterrier et al, ; Xu, Guerra, Lee, & Vierling, ). A GSNO reductase is itself a target for S‐nitrosylation, thus representing an autoregulatory loop (Guerra, Ballard, Truebridge, & Vierling, ; Tichá, Lochman, Činčalová, Luhová, & Petřivalský, ; Zhan et al, ). Another redox mechanism, based on the action of thioredoxins such as TRX h3 and h5, reducing S‐nitrosylated proteins seems to be also relevant to control S‐nitrosylation‐related signaling specifically in plant immunity to pathogens (Kneeshaw, Gelineau, Tada, Loake, & Spoel, ; Tada et al, ).…”

Section: Relevant No Synthesis Pathways In Plantsmentioning

confidence: 99%

Present knowledge and controversies, deficiencies, and misconceptions on nitric oxide synthesis, sensing, and signaling in plants

León

Costa-Broseta

2019

Plant Cell & Environment

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After 30 years of intensive work, nitric oxide (NO) has just started to be characterized as a relevant regulatory molecule on plant development and responses to stress. Its reactivity as a free radical determines its mode of action as an inducer of posttranslational modifications of key target proteins through cysteine S‐nitrosylation and tyrosine nitration. Many of the NO‐triggered regulatory actions are exerted in tight coordination with phytohormone signaling. This review not only summarizes and updates the information accumulated on how NO is synthesized, sensed, and transduced in plants but also makes emphasis on controversies, deficiencies, and misconceptions that are hampering our present knowledge on the biology of NO in plants. The development of noninvasive accurate tools for the endogenous NO quantitation as well as the implementation of genetic approaches that overcome misleading pharmacological experiments will be critical for getting significant advances in better knowledge of NO homeostasis and regulatory actions in plants.

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Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues

Cited by 36 publications

References 33 publications

Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂

Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂

Treatment of nitric oxide supplemented with nitrogen and sulfur regulates photosynthetic performance and stomatal behavior in mustard under salt stress

Present knowledge and controversies, deficiencies, and misconceptions on nitric oxide synthesis, sensing, and signaling in plants

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Redox regulation of plant S-nitrosoglutathione reductase activity through post-translational modifications of cysteine residues

Cited by 36 publications

References 33 publications

Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H2O2

Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H2O2

Treatment of nitric oxide supplemented with nitrogen and sulfur regulates photosynthetic performance and stomatal behavior in mustard under salt stress

Present knowledge and controversies, deficiencies, and misconceptions on nitric oxide synthesis, sensing, and signaling in plants

Contact Info

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Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂

Glutathione‐dependent denitrosation of GSNOR1 promotes oxidative signalling downstream of H₂O₂