Redox Regulation of STAT1 and STAT3 Signaling

Butturini, Elena; Prati, Alessandra Carcereri de; Mariotto, Sofia

doi:10.3390/ijms21197034

Cited by 112 publications

(61 citation statements)

References 90 publications

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“…p value 0.04). Stat3 is phosphorylated and translocated into the nucleus (Figure 5A–C ), despite potential redox interference in Stat3 phosphorylation 67 by increased amounts of Xdh. In addition, mRNA encoding Timp1, which suppresses matrix degradation is increased almost twofold (Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

The butyrophilin 1a1 knockout mouse revisited: Ablation ofBtn1a1leads to concurrent cell death and renewal in the mammary epithelium during lactation

et al. 2021

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Butyrophilin 1A1 (BTN1A1) is implicated in the secretion of lipid droplets from mammary epithelial cells as a membrane receptor, which forms a secretion complex with the redox enzyme, xanthine oxidoreductase (XDH). The first evidence that BTN1A1 functions in this process was the generation of Btn1a1−/− mouse lines, in which lipid secretion was disrupted and large unstable droplets were released into alveolar spaces with fragmented surface membranes. We have revisited one of these mutant mouse lines using RNAseq and proteomic analysis to assess the consequences of ablating the Btn1a1 gene on the expression of other genes and proteins. Disruption of intact Btn1a1 protein expression led to a large build‐up of Xdh in the cytoplasm, induction of acute phase response genes and Lif‐activation of Stat3 phosphorylation. At peak lactation, approx. 10% of the cells were dying, as assessed by TUNEL‐analysis of nuclear DNA. Possible cell death pathways included expression of caspase 8 and activated caspase 3, autophagy, Slc5a8‐mediated inactivation of survivin (Birc5), and pStat3‐mediated lysosomal lysis, the latter of which is the principal death route in involuting wild type cells. Milk secretion was prolonged by renewal of the secretory epithelium, as evidenced by the upregulation of Ki67 in approx. 10% of cell nuclei and expression of cyclins and Fos/Jun. These data highlight the plasticity of the mammary epithelium and the importance of functional BTN1A1 expression for maintenance of terminally differentiated secretory cells and optimal milk production throughout lactation.

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Section: Discussionmentioning

confidence: 99%

The butyrophilin 1a1 knockout mouse revisited: Ablation ofBtn1a1leads to concurrent cell death and renewal in the mammary epithelium during lactation

et al. 2021

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“…STAT1 and STAT3 are not only significantly up-regulated, but also have more binding sites ( STAT1:6 , STAT3:7 ) at the promoter region of Mb in our results. STAT1 and STAT3 are signal transducers and transcriptional activators that play an important role in regulating cell proliferation, differentiation and migration [ 39 , 40 ]. Grohmann and others have shown that oxidative stress caused by obesity inhibits the activity of protein tyrosine phosphatase and leads to the activation of STAT1 [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Prediction and Experimental Verification of a Hierarchical Transcription Factor Regulatory Network of Porcine Myoglobin (Mb)

Yuan

Liu

et al. 2021

Animals

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Myoglobin is a key chemical component that determines meat’s color and affects consumers’ purchase intentions. In this work, we firstly identified the promoter sequence of the Mb gene from the primary assembly of high-throughput genome sequencing in pigs, and predicted its potential transcription factors by LASAGNA. Through the data mining of the mRNA expression profile of longissimus dorsi muscle of different pig breeds, we constructed a hierarchical interplay network of Mb-TFs (Myoglobin-Transcription Factors), consisting of 16 adaptive transcription factors and 23 secondary transcription factors. The verification of gene expression in longissimus dorsi muscle showed that the Mb mRNA and encoded protein were significantly (p < 0.05) more abundant in Bama pigs than Yorkshire pigs. The qRT-PCR (Real-Time Quantitative Reverse Transcription PCR) validation on genes of the Mb-TFs network showed that FOS, STAT3, STAT1, NEFL21, NFE2L2 and MAFB were significant positive regulatory core transcription factors of Mb-TFs network in Bama pigs, whereas ATF3 was the secondary transcription factor most responsible for the activation of the above transcription factors. Our study provides a new strategy to unravel the mechanism of pork color formation, based on public transcriptome and genome data analysis.

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“…# p < .01, as compared to the cells exposed to PM alone. HGFs, human gingival fibroblasts; PM, particulate matter also a redox-sensitive protein 38. Hence, this study supposed that STAT1 can be a crucial node linking ROS signaling pathway and VCAM-1 expression in HGFs, leading to monocyte adhesion.…”

mentioning

confidence: 82%

Surfactin reduces particulate matter–induced VCAM‐1–dependent monocyte adhesion in human gingival fibroblasts by increasing Nrf2‐dependent HO‐1 expression

Huang

Wee

et al. 2021

J of Periodontal Research

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Background and objectives:The mechanisms of particulate matter (PM) toxicity involve the generation of ROS and upregulation of proinflammatory molecules. Nrf2 is a multifunctional cytoprotective transcription factor that regulates the expression of various antioxidant, anti-inflammatory, and detoxifying molecules, such as HO-1.As surfactin has potential to induce Nrf2 activation and HO-1 expression, this study aimed to investigate the anti-inflammatory effects of surfactin on PM-exposed human gingival fibroblasts (HGFs) and signaling pathways engaged by surfactin. Materials and methods: Human gingival fibroblasts were challenged by PM with or without surfactin pretreatment. The expression of Nrf2, HO-1, VCAM-1, and other molecules was determined by western blot, real-time PCR, or ELISA. Human monocytic THP-1 cells labeled with fluorescent reagent were added to HGFs, and the cell adhesion was assessed. ROS generation and NADPH oxidase activity were also measured. The involvement of Nrf2/HO-1 and ROS signaling pathways was investigated by treating HGFs with specific pathway interventions, genetically or pharmacologically.One dose of surfactin was given to mice before PM treatment to explore its in vivo effect on VCAM-1 expression in gingival tissues.

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Redox Regulation of STAT1 and STAT3 Signaling

Cited by 112 publications

References 90 publications

The butyrophilin 1a1 knockout mouse revisited: Ablation ofBtn1a1leads to concurrent cell death and renewal in the mammary epithelium during lactation

The butyrophilin 1a1 knockout mouse revisited: Ablation ofBtn1a1leads to concurrent cell death and renewal in the mammary epithelium during lactation

Prediction and Experimental Verification of a Hierarchical Transcription Factor Regulatory Network of Porcine Myoglobin (Mb)

Surfactin reduces particulate matter–induced VCAM‐1–dependent monocyte adhesion in human gingival fibroblasts by increasing Nrf2‐dependent HO‐1 expression

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