Redox status of patients before cardiac surgery

Schuh, Anna Katharina; Sheybani, Babak; Jortzik, Esther; Niemann, Bernd; Wilhelm, Jochen; Boening, Andreas; Becker, Katja

doi:10.1080/13510002.2017.1418620

Cited by 8 publications

(5 citation statements)

References 56 publications

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“…In humans, this correlation was related to the serum content of uric acid, one of the major components determining the antioxidant status in human serum (Erel, 2004;Jansen and Ruskovska, 2013), as previously in vitro observed by Benzie and Strain (1996) and Re et al (1999). The lack of correlation between TAC and TAA in our data could be explained by the lower uric acid content in goat compared to humans (Silanikove et al, 1996). Thus, the assessed antioxidant assays were less influenced by the uric acid contribution, which represents 60 % of the antioxidant capacity measured by FRAP assay in human samples (Cao and Prior, 1998).…”

Section: Discussionsupporting

confidence: 82%

Assessment of total (anti)oxidant status in goat kids

Gualandi

Fazio

2021

Arch. Anim. Breed.

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Abstract. The redox potential of goat serum was assessed by different spectrophotometric assays. Among them, three methods are commonly applied for the evaluation of the oxidative (reactive oxygen metabolites, ROMs, and total oxidant status, TOS) and nitrosative (NO⚫ metabolites, NOx) stress, and four methods for the evaluation of the antioxidant status: the total antioxidant capacity (TAC) based on the ferric reducing ability of plasma (FRAP), the total antioxidant activity (TAA) based on the reduction of the coloured ABTS⚫+ radical cation, the free radical scavenging activity (FRSA) based on the reduction of the purple DPPH⚫, and the total thiol levels (TTLs) based on their interaction with DTNB to form a highly coloured anion. Besides, myeloperoxidase (MPO) and ceruloplasmin oxidase (CP) activities were also assessed. Except for TAA, analytical data showed a great inter-individual variation for both oxidant and antioxidant assays. ROMs were strongly correlated with CP, while TOS with MPO and TAC. Furthermore, a tendency between TOS and FRSA was shown. NOx was correlated with TAC and TAA, and a tendency with TOS was shown. No correlations appeared among the antioxidant assays, even if a tendency between TAC and TAA was evidenced, but TAC was correlated with MPO activity. The observed correlation between ROMs and CP is discussed as a possible analytical interference. The absence of correlation among the antioxidant biomarkers suggests the simultaneous use of a panel of tests to verify any changes in the redox balance, mainly in livestock in which reference values for each biomarker are lacking.

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Section: Discussionsupporting

confidence: 82%

Assessment of total (anti)oxidant status in goat kids

Gualandi

Fazio

2021

Arch. Anim. Breed.

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“…Extensive increase in ROS levels results in oxidative stress. Correspondingly, monitoring the redox status of patients before and after cardiovascular surgical procedures by determining redox parameters, i.e., total antioxidant status, total glutathione concentrations, glutathione peroxidase activity, and both nitrite and nitrate concentrations in the peripheral blood of patients, can be of great impact (Schuh et al, 2018) for optimal treatment in the postoperative time period. In future clinical studies, when PS is used, a combination of mitochondria-specific antioxidants or ROS scavengers to reverse the side effect of PS should be evaluated.…”

Section: Discussionmentioning

confidence: 99%

Protamine Sulfate Induces Mitochondrial Hyperpolarization and a Subsequent Increase in Reactive Oxygen Species Production

Ramzan

Michels

Weber

et al. 2019

J Pharmacol Exp Ther

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Protamine sulfate (PS) is widely used in heart surgery as an antidote for heparin, albeit its pharmacological effects are not fully understood and applications are often accompanied by unwanted side effects. Here we show the effect of PS on mitochondrial bioenergetics profile resulting in mitochondrial reactive oxygen species (ROS) production. Polarographic measurements were performed in parallel to membrane potential and ROS measurements by FACS analyzer using tetramethylrhodamine ethyl ester and MitoSOX fluorescent dyes, respectively. PS inhibited intact rat heart mitochondrial respiration (stimulated by ADP) to 76% (P , 0.001) from the baseline of 51.6 6 6.9 to 12.4 6 2.3 nmol O 2 ×min 21 ×ml 21 . The same effect was found when respiration was inhibited by antimycin A (101.0 6 8.9 vs. 38.0 6 9.9 nmol O 2 ×min 21 ×ml 21 , P , 0.001) and later stimulated by substrates of cytochrome oxidase (CytOx) i.e., ascorbate and tetramethyl phenylene diamine, suggesting that PS exerted its effect through inhibition of CytOx activity. Furthermore, the inhibition of mitochondrial respiration by PS was concentration dependent and accompanied by hyperpolarization of the mitochondrial membrane potential (Dc m ), i.e., 18% increase at 50 mg/ml and an additional 3.3% increase at 250 mg/ml PS compared with control. This effect was associated with a strong consequent increase in the production of ROS, i.e., 85% and 88.6% compared with control respectively. We propose that this excessive increase in ROS concentrations results in mitochondrial dysfunction and thus might relate to the "protamine reaction," contributing to the development of various cardiovascular adverse effects.

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“…It is likely that prolonged administration of OT in CAD patients with elevated basal angiotensin-II levels accelerated angiotensin-II-induced hypertension and renal damage (Gu et al, 2016). Since elevated basal levels of angiotensin-II is common among patients with CAD complications (Schuh et al, 2017), alternative approaches of OT application should be considered.…”

Section: Limitation For Therapeutic Use Of Otmentioning

confidence: 99%

Therapeutic Potential of Oxytocin in Atherosclerotic Cardiovascular Disease: Mechanisms and Signaling Pathways

Wang

Yang

et al. 2019

Front. Neurosci.

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Coronary artery disease (CAD) is a major cardiovascular disease responsible for high morbidity and mortality worldwide. The major pathophysiological basis of CAD is atherosclerosis in association with varieties of immunometabolic disorders that can suppress oxytocin (OT) receptor (OTR) signaling in the cardiovascular system (CVS). By contrast, OT not only maintains cardiovascular integrity but also has the potential to suppress and even reverse atherosclerotic alterations and CAD. These protective effects of OT are associated with its protection of the heart and blood vessels from immunometabolic injuries and the resultant inflammation and apoptosis through both peripheral and central approaches. As a result, OT can decelerate the progression of atherosclerosis and facilitate the recovery of CVS from these injuries. At the cellular level, the protective effect of OT on CVS involves a broad array of OTR signaling events. These signals mainly belong to the reperfusion injury salvage kinase pathway that is composed of phosphatidylinositol 3-kinase-Akt-endothelial nitric oxide synthase cascades and extracellular signal-regulated protein kinase 1/2. Additionally, AMP-activated protein kinase, Ca 2+ /calmodulin-dependent protein kinase signaling and many others are also implicated in OTR signaling in the CVS protection. These signaling events interact coordinately at many levels to suppress the production of inflammatory cytokines and the activation of apoptotic pathways. A particular target of these signaling events is endoplasmic reticulum (ER) stress and mitochondrial oxidative stress that interact through mitochondria-associated ER membrane. In contrast to these protective effects and machineries, rare but serious cardiovascular disturbances were also reported in labor induction and animal studies including hypotension, reflexive tachycardia, coronary spasm or thrombosis and allergy. Here, we review our current understanding of the protective effect of OT against varieties of atherosclerotic etiologies as well as the approaches and underlying mechanisms of these effects. Moreover, potential cardiovascular disturbances following OT application are also discussed to avoid unwanted effects in clinical trials of OT usages.

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Redox status of patients before cardiac surgery

Cited by 8 publications

References 56 publications

Assessment of total (anti)oxidant status in goat kids

Assessment of total (anti)oxidant status in goat kids

Protamine Sulfate Induces Mitochondrial Hyperpolarization and a Subsequent Increase in Reactive Oxygen Species Production

Therapeutic Potential of Oxytocin in Atherosclerotic Cardiovascular Disease: Mechanisms and Signaling Pathways

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