Redox status of the testes and sperm of rats following exposure to 2,5-hexanedione

Adedara, Isaac A.; Abolaji, Amos O.; Odion, Blessing E.; Omoloja, Abiola A.; Okwudi, Isioma J.

doi:10.1080/13510002.2015.1107312

Cited by 19 publications

(13 citation statements)

References 36 publications

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“…Glutathione S ‐transferase has dual functions in antioxidant defense and xenobiotic detoxication, loss of the enzyme activity could lead to increased susceptibility of testis to tebuconazole toxicity and render testis a major target organ. Along this line, decrease of testicular glutathione S ‐transferase activity has been reported in male rats treated with reproductive toxicants triazine and 2,5‐hexanedione and functionalized multi‐walled carbon nanotubes …”

Section: Discussionmentioning

confidence: 85%

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

Yang

Liu

Liao

et al. 2018

Environmental Toxicology

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The major objective of the present study was to determine the ability of a triazole fungicide tebuconazole to induce cytochrome P450-dependent monooxygenases, oxidative stress, and endocrine-disrupting activity using male rats treated with tebuconazole at 10, 25, and 50 mg/kg p.o. once daily for 28 days. In liver, tebuconazole dose-dependently increased microsomal contents of cytochrome P450 and cytochrome b and the activities of NADPH-cytochrome P450 reductase, 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, pentoxyresorufin O-dealkylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase. In kidney, tebuconazole increased 7-ethoxycoumarin O-deethylase activity without affecting other monooxygenase activities. In marked contrast to liver and kidney, tebuconazole decreased testicular 7-ethoxyresorufin O-deethylase, methoxyresorufin O-demethylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythromycin N-demethylase activities. The results of immunoblot analysis of liver microsomes of controls and tebuconazole-treated rats revealed that tebuconazole induced CYP1A1/2, CYP2B1/2, CYP2E1, and CYP3A proteins in liver. Additions of tebuconazole to liver microsomes inhibited microsomal 7-ethoxycoumarin O-deethylase activity in vitro (IC = 1.50-1.69 µM). Treatment of rats with tebuconazole decreased glutathione content and increased glutathione S-transferase, superoxide dismutase, catalase, and glutathione peroxidase activities in liver; increased superoxide dismutase activities in kidney and testis; but decreased glutathione S-transferase activity in testis. Treatments with tebuconazole decreased serum testosterone concentration and cauda epididymal sperm count. The present study demonstrates that tebuconazole induces a multiplicity of CYPs and oxidative stress in liver; inhibits testicular P450 and glutathione S-transferase activities; and produces anti-androgenic effects in male rats.

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Section: Discussionmentioning

confidence: 85%

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

Yang

Liu

Liao

et al. 2018

Environmental Toxicology

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“…25,26 Testicular weight is an important marker of male reproductive toxicity whereas the RTW is an established indicator of degenerative changes including swelling, atrophy or hypertrophy. 47 The decrease in the absolute and RTWs observed in mice exposed to DSS alone and SZ alone indicates testicular atrophy mediated by DSS-induced colitis and SZ, respectively. Our observation on the reduction in testes weight is in contrast to previous observations in acute testicular effect of SZ administration in Sprague Dawley rats for 14 days.…”

Section: Discussionmentioning

confidence: 97%

6-Gingerol improves testicular function in mice model of chronic ulcerative colitis

et al. 2017

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The persistent inflammation and oxidative stress at the local site in ulcerative colitis reportedly extend to the testes via systemic circulation resulting in testicular dysfunction. The influence of 6-gingerol (6G), a phenolic compound isolated from Zingiber officinale, on colitis-mediated testicular dysfunction in mice was investigated in this study. Chronic ulcerative colitis was induced in mice using 2.5% dextran sulfate sodium (DSS) in drinking water for three cycles. Each cycle consisted of 7 consecutive days of exposure to DSS-treated water followed by 14 consecutive days of normal drinking water. 6G (100 mg/kg) or sulfasalazine (SZ; 100 mg/kg) was orally administered alone or in combination with DSS-treated water during the three cycles. SZ served as standard reference drug for colitis in this study. 6G significantly prevented the incidence of rectal bleeding, decrease in the body weight gain and colon mass index in DSS-exposed mice. 6G significantly prevented colitis-mediated decreases in luteinizing hormone, follicle-stimulating hormone and testosterone and decreases oxidative stress indices, pro-inflammatory cytokines and caspase-3 activity with concomitant augmentation of antioxidant enzymes activities, sperm characteristics, marker enzymes of testicular function and histoarchitecture in DSS-exposed mice. 6G exerted protective influence against ulcerative colitis-induced testicular damage via mechanisms involving its antioxidant and anti-inflammatory properties.

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“…Data obtained from this study indicated that GSC treatment in rats did not elicit changes on absolute weights and organosomatic indices of both testis and epididymis. Since the gross weight and organosomatic indices of the testes mirror the condition of the organ following exposure to xenobiotics, the lack of discernible effect of GSC on the germ cells could be inferred from the data [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Histomorphological and Redox Delineations in the Testis and Epididymis of Albino Rats Fed with Green-Synthesized Cellulose

Otuechere

Adewuyi

Adebayo

et al. 2020

Biology

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It has also become increasingly necessary to diversify the production of cellulose for biomedical applications. In this study, cellulose-green-synthesized from Sesamum indicum (GSC)—was administered orally to rats for 14 days as follows: control, 100, 200 and 400 mg/kg GSC. The impact of GSC on the antioxidant status and histomorphology of the testes and epididymis were studied. GSC had no effects on organ weights and organosomatic indices. In the testes, GSC caused nonsignificant changes in superoxide dismutase, catalase, reduced glutathione and nitric oxide levels, whereas it significantly decreased glutathione peroxidase and malondialdehyde levels. In the epididymis, GSC significantly decreased superoxide dismutase and nitric oxide levels, but caused a significant increase in glutathione peroxidase and reduced glutathione levels. Furthermore, at ×200 magnification, testicular morphology appeared normal at all doses, however, extravasation of the germinal epithelium of the epididymis was observed at doses of 200 and 400 mg/kg GSC. Conversely, at ×400 magnification, spermatogenic arrest (testes) and chromatolytic alterations (epididymis) were observed at the higher doses (200 and 400 mg/kg GSC). This study reports on the effect of green-synthesized cellulose on testicular and epididymal histology and redox status and further extends the frontiers of research on cellulose.

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Redox status of the testes and sperm of rats following exposure to 2,5-hexanedione

Abstract: 2,5-HD exposure impaired testicular function and sperm characteristics by disruption of the antioxidant systems and consequently, increased oxidative stress in the treated rats.

Cited by 19 publications

References 36 publications

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

Effects of tebuconazole on cytochrome P450 enzymes, oxidative stress, and endocrine disruption in male rats

6-Gingerol improves testicular function in mice model of chronic ulcerative colitis

Histomorphological and Redox Delineations in the Testis and Epididymis of Albino Rats Fed with Green-Synthesized Cellulose

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