Redox status parameters and PBMC membrane fluidity in diabetes mellitus

Abstract

AbbreviationsATP/ADP = adenosine-triphosphate/adenosine-diphosphate; ESP = erythrocytes' susceptibility to lipid peroxidation; FPG = fasting plasma glucose; G6PDH = glucose-6-phosphate dehydrogenase; HbA1c = glycated hemoglobin; IDDM = insulin dependent diabetes mellitus; LDL = low density lipoproteins; MDA = malondialdehyde; NADP + /NADPH = nicotinamide adenine dinucleotide phosphate oxidized/reduced; NIDDM = non-insulin dependent diabetes mellitus; NO = nitric oxide; NOS = nitric oxide synthase; PBMC = peripheral blood mononuclear cell; PBS = phosphate-buffered saline; PMS = phenazine methosulfate; RBC = red blood cell; ROS = reactive oxygen species; SOD = superoxide dismutase; TC = total cholesterol; TG = triglycerides; TMA-DPH = 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene ptoluensulfonate The present study aims at finding correlations between certain biochemical and biophysical blood parameters for diabetes patients, focusing on the antioxidant status of the red blood cells and the membrane fluidity of peripheral blood mononuclear cells (PBMC), the endothelial function and the risk of stable interaction between the leucocytes and the endothelium. For that purpose we evaluated blood samples from 32 diabetes patients compared to a control group of 10 subjects for erythrocytes' enzymatic activity of glucose-6-phosphate dehydrogenase and of superoxide dismutase, their susceptibility to lipid peroxidation, the plasma nitric oxide stable end products level and the PBMC membrane fluidity. Our results showed that the erythrocytes' antioxidant mechanisms and the PBMC membrane fluidity are impaired under chronic hyperglycemic conditions. Since microvascular complications of diabetes are mainly determined by redox mechanisms, the evaluation of these parameters might help in characterizing the risk of vascular complication for diabetes patients.

Quercetin and Epigallocatechin Gallate Induce in Vitro a Dose-Dependent Stiffening and Hyperpolarizing Effect on the Cell Membrane of Human Mononuclear Blood Cells

“…The PBMCs were separated using the gradient density method, with Hystopaque 1077 [ 48 ]. Separated PBMCs (monocytes and lymphocytes) were washed three times with RPMI 1640 medium, standardized at 10 5 cells/mL and used for the spectrofluorimetric assay [ 49 , 50 ]. Platelet rich plasma samples were used for the biochemical evaluation.…”