Network-on-Chip (NoC) is the ideal interconnection architecture for many-core systems due to its superior scalability and performance. An NoC must deliver critical messages from a realtime application within specific deadlines. A violation of this requirement may compromise the entire system operation. Therefore, a series of experiments considering worst-case scenarios must be conducted to verify if deadlines can be satisfied. However, simulation-based experiments are time-consuming, and one alternative is schedulability analysis. In this context, this work proposes a schedulability analysis to accelerate design space exploration in real-time applications on NoC-based systems. The proposed worstcase analysis estimates the maximum latency of traffic flows assuming direct and indirect blocking. Besides, we consider the size of buffers to reduce the analysis' pessimism. We also present an extension of the analysis, including self-blocking. We conduct a series of experiments to evaluate the proposed analysis using a cycle-accurate simulator. The experimental results show that the proposed solution presents tighter results and runs four orders of magnitude faster than the simulation.INDEX TERMS Network-on-Chip, timing analysis, design space exploration.