Reduced abundance of the CYP6CY3-targeting let-7 and miR-100 miRNAs accounts for host adaptation of Myzus persicae nicotianae

Peng, Tianfei; Pan, Yiou; Gao, Xiwu; Xi, Jinghui; Zhang, Lei; Ma, Kangsheng; Wu, Yongqiang; Zhang, Juhong; Shang, Qingli

doi:10.1016/j.ibmb.2016.06.002

Cited by 55 publications

(55 citation statements)

References 43 publications

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“…bicolor , we found enrichment of genes for gene silencing by RNA. This was previously shown on some hosts of Myzus persicae (in which case miRNA activity is negatively correlated with the abundance of a P450 enzyme) [55]. In lobate lac scales on P .…”

Section: Discussionsupporting

confidence: 65%

Gene expression plasticity across hosts of an invasive scale insect species

et al. 2017

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For plant-eating insects, we still have only a nascent understanding of the genetic basis of host-use promiscuity. Here, to improve that situation, we investigated host-induced gene expression plasticity in the invasive lobate lac scale insect, Paratachardina pseudolobata (Hemiptera: Keriidae). We were particularly interested in the differential expression of detoxification and effector genes, which are thought to be critical for overcoming a plant’s chemical defenses. We collected RNA samples from P. pseudolobata on three different host plant species, assembled transcriptomes de novo, and identified transcripts with significant host-induced gene expression changes. Gene expression plasticity was pervasive, but the expression of most detoxification and effector genes was insensitive to the host environment. Nevertheless, some types of detoxification genes were more differentially expressed than expected by chance. Moreover, we found evidence of a trade-off between expression of genes involved in primary and secondary metabolism; hosts that induced lower expression of genes for detoxification induced higher expression of genes for growth. Our findings are largely consonant with those of several recently published studies of other plant-eating insect species. Thus, across plant-eating insect species, there may be a common set of gene expression changes that enable host-use promiscuity.

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Section: Discussionsupporting

confidence: 65%

Gene expression plasticity across hosts of an invasive scale insect species

et al. 2017

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“…To prove the concept, they further showed that miR656a-3p binds to the 3’-UTR of the transcript encoding the putative detoxification enzyme CYP6J1, promoting its degradation; expression of miR656a-3p is low when aphids are fed on a diet containing 2-tridecanone, tannic acid or quercetin that may result in higher CYP6J1 levels that may be involved in the detoxification of these compounds, though these final steps were not shown. Adult aphids of the tobacco specialist Myzus persicae own two miRNA species, let-7 and miR-100, that are predicted to negatively regulate transcript levels of Cyp6Cy3 encoding a nicotine detoxification enzyme [ 56 ]. The nicotine-tolerant M. persicae subspecies nicotianae has lower endogenous levels of these miRNAs and is thus more able to cope with nicotine.…”

Section: Plant-insect Interactionsmentioning

confidence: 99%

miRNA-Mediated Interactions in and between Plants and Insects

Wong

Wang

et al. 2018

IJMS

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Our understanding of microRNA (miRNA) regulation of gene expression and protein translation, as a critical area of cellular regulation, has blossomed in the last two decades. Recently, it has become apparent that in plant-insect interactions, both plants and insects use miRNAs to regulate their biological processes, as well as co-opting each others’ miRNA systems. In this review article, we discuss the current paradigms of miRNA-mediated cellular regulation and provide examples of plant-insect interactions that utilize this regulation. Lastly, we discuss the potential biotechnological applications of utilizing miRNAs in agriculture.

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“…Several studies have reported miRNA-mediated regulation of cytochrome P450 expression. Recent work conducted in the tobacco aphid Myzus persicae nicotianae (Blackman) showed that expression of CYP6CY3, a cytochrome P450 previously associated with neonicotinoid resistance [ 31 ], was regulated by miR-100 [ 32 ]. The present study highlighted downregulation of miR-100-5p levels in imidacloprid-treated CPBs, supporting a potential role for the miR-100-cytochrome P450 axis in the imidacloprid response.…”

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid

Morin

Lyons

Crapoulet

et al. 2017

IJMS

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The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is a significant pest of potato plants that has been controlled for more than two decades by neonicotinoid imidacloprid. L. decemlineata can develop resistance to this agent even though the molecular mechanisms underlying this resistance are not well characterized. MicroRNAs (miRNAs) are short ribonucleic acids that have been linked to response to various insecticides in several insect models. Unfortunately, the information is lacking regarding differentially expressed miRNAs following imidacloprid treatment in L. decemlineata. In this study, next-generation sequencing and quantitative real-time polymerase chain reaction (qRT-PCR) were used to identify modulated miRNAs in imidacloprid-treated versus untreated L. decemlineata. This approach identified 33 differentially expressed miRNAs between the two experimental conditions. Of interest, miR-282 and miR-989, miRNAs previously shown to be modulated by imidacloprid in other insects, and miR-100, a miRNA associated with regulation of cytochrome P450 expression, were significantly modulated in imidacloprid-treated beetles. Overall, this work presents the first report of a miRNA signature associated with imidacloprid exposure in L. decemlineata using a high-throughput approach. It also reveals interesting miRNA candidates that potentially underly imidacloprid response in this insect pest.

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Reduced abundance of the CYP6CY3-targeting let-7 and miR-100 miRNAs accounts for host adaptation of Myzus persicae nicotianae

Cited by 55 publications

References 43 publications

Gene expression plasticity across hosts of an invasive scale insect species

Gene expression plasticity across hosts of an invasive scale insect species

miRNA-Mediated Interactions in and between Plants and Insects

Identification of Differentially Expressed miRNAs in Colorado Potato Beetles (Leptinotarsa decemlineata (Say)) Exposed to Imidacloprid

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