Reduced activity of<i>Arabidopsis</i>chromosome-cohesion regulator gene<i>CTF7/ECO1</i>alters cytosine methylation status and retrotransposon expression

Bolaños-Villegas, Pablo; Jauh, Guang‐Yuh

doi:10.1080/15592324.2015.1013794

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…The loaded cohesin requires an additional ‘establishment’ step that involves the acetylation of a pair of lysine residues within Smc3 by the evolutionarily conserved cohesin acetyltransferases ( 9–14 ). In budding yeast, Eco1 is the acetyltransferase that establishes sister chromatid cohesion during DNA replication ( 15–18 ). In addition, Eco1 mutation that genocopies Roberts syndrome has impaired DNA damage repair, reduced ribosomal DNA (rDNA) transcription and aberrant gene expression pattern ( 17 , 19 ).…”

Section: Introductionmentioning

confidence: 99%

The cohesion establishment factor Esco1 acetylates α-tubulin to ensure proper spindle assembly in oocyte meiosis

Cui

et al. 2018

Nucleic Acids Research

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Esco1 has been reported to function as a cohesion establishment factor that mediates chromosome cohesion and segregation in mitotic cells. However, its exact roles in meiosis have not been clearly defined. Here, we document that Esco1 is expressed and localized to both the nucleus and cytoplasm during mouse oocyte meiotic maturation. Depletion of Esco1 by siRNA microinjection causes the meiotic progression arrest with a severe spindle abnormality and chromosome misalignment, which is coupled with a higher incidence of the erroneous kinetochore–microtubule attachments and activation of spindle assembly checkpoint. In addition, depletion of Esco1 leads to the impaired microtubule stability shown by the weakened resistance ability to the microtubule depolymerizing drug nocodazole and the decreased level of acetylated α-tubulin. Conversely, overexpression of Esco1 causes hyperacetylation of α-tubulin and spindle defects. Moreover, we find that Esco1 binds to α-tubulin and is required for its acetylation. The reduced acetylation level of α-tubulin in Esco1-depleted oocytes can be restored by the ectopic expression of exogenous wild-type Esco1 but not enzymatically dead Esco1-G768D. Purified wild-type Esco1 instead of mutant Esco1-G768D acetylates the synthesized peptide of α-tubulin in vitro. Collectively, our data assign a novel function to Esco1 as a microtubule regulator during oocyte meiotic maturation beyond its conventional role in chromosome cohesion.

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Section: Introductionmentioning

confidence: 99%

The cohesion establishment factor Esco1 acetylates α-tubulin to ensure proper spindle assembly in oocyte meiosis

Cui

et al. 2018

Nucleic Acids Research

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“…In Arabidopsis , defects in the function of SMC2 (2 genes) or SMC4 (3 genes) affect segregation of mitotic and meiotic chromosomes ( Sakamoto et al, 2019 ). Also, defects in Arabidopsis SMC4 (At5g48600, also known as AtCAP-C ) cause deregulation in the expression of centromeric and pericentric transposable elements (such as COPIA ), reduced CG methylation, and chromocenter decondensation (see Table 1 ), phenotypes reminiscent of those reported for ECO1/CTF7 mutants ( Bolaños-Villegas and Jauh, 2015 ; De et al, 2016 ) and perhaps suggesting a regulatory link between SMC4 acetylation and maintenance of the genome.…”

Section: Engineering Systems Redundancy: Complementary Roles Of Cohesmentioning

confidence: 98%

“…Evidence also indicates that SMC4/condensin is required for repression of a wide range of methylated genes subject to conditional expression (Wang et al, 2017). Gene examples are embryo and pollen vegetative cell-specific GAMETE EXPRESSED-PROTEIN 1 (GEX1, At5g5590; Alandete-Saez et al, 2011), pollen tube-expressed POLYMERASE DELTA 4 (POLD4, At1g09815; Wang et al, 2008), radiation-response factors such as GAMMA-IRRADIATION AND MITOMYCIN C INDUCED 1 (GMI1, At5g24280; Böhmdorfer et al, 2011), and translesion-synthesis/homologous recombination factor RECQ3 (At4g35740; Kobbe et al, 2009). Thus, breeding for condensin alleles may confer resistance to mitotic DNA damage caused by UVB and Al 3+ and boost allele reshuffling via homologous recombination.…”

Section: Engineering Systems Redundancy: Complementary Roles Of Cohesmentioning

confidence: 99%

The Role of Structural Maintenance of Chromosomes Complexes in Meiosis and Genome Maintenance: Translating Biomedical and Model Plant Research Into Crop Breeding Opportunities

Bolaños-Villegas

2021

Front. Plant Sci.

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Cohesin is a multi-unit protein complex from the structural maintenance of chromosomes (SMC) family, required for holding sister chromatids together during mitosis and meiosis. In yeast, the cohesin complex entraps sister DNAs within tripartite rings created by pairwise interactions between the central ring units SMC1 and SMC3 and subunits such as the α-kleisin SCC1 (REC8/SYN1 in meiosis). The complex is an indispensable regulator of meiotic recombination in eukaryotes. In Arabidopsis and maize, the SMC1/SMC3 heterodimer is a key determinant of meiosis. In Arabidopsis, several kleisin proteins are also essential: SYN1/REC8 is meiosis-specific and is essential for double-strand break repair, whereas AtSCC2 is a subunit of the cohesin SCC2/SCC4 loading complex that is important for synapsis and segregation. Other important meiotic subunits are the cohesin EXTRA SPINDLE POLES (AESP1) separase, the acetylase ESTABLISHMENT OF COHESION 1/CHROMOSOME TRANSMISSION FIDELITY 7 (ECO1/CTF7), the cohesion release factor WINGS APART-LIKE PROTEIN 1 (WAPL) in Arabidopsis (AtWAPL1/AtWAPL2), and the WAPL antagonist AtSWITCH1/DYAD (AtSWI1). Other important complexes are the SMC5/SMC6 complex, which is required for homologous DNA recombination during the S-phase and for proper meiotic synapsis, and the condensin complexes, featuring SMC2/SMC4 that regulate proper clustering of rDNA arrays during interphase. Meiotic recombination is the key to enrich desirable traits in commercial plant breeding. In this review, I highlight critical advances in understanding plant chromatid cohesion in the model plant Arabidopsis and crop plants and suggest how manipulation of crossover formation during meiosis, somatic DNA repair and chromosome folding may facilitate transmission of desirable alleles, tolerance to radiation, and enhanced transcription of alleles that regulate sexual development. I hope that these findings highlight opportunities for crop breeding.

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“…Furthermore, ctf7 mutant plants exhibit a severe loss of sister chromatid cohesion during mitosis and meiosis as well as significantly reduced localization of cohesin onto chromosomes ( Bolaños-Villegas et al, 2013 ). The absence of ECO1/CTF7 impairs cytosine methylation, especially CG methylation ( Bolaños-Villegas and Jauh, 2015 ). In addition, genes involved in HR are upregulated, which suggests defects in DNA repair ( Bolaños-Villegas et al, 2013 ).…”

Section: Cohesion Establishment and Maintenancementioning

confidence: 99%

In Favor of Establishment: Regulation of Chromatid Cohesion in Plants

Bolaños-Villegas

Pradillo

et al. 2017

Front. Plant Sci.

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In eukaryotic organisms, the correct regulation of sister chromatid cohesion, whereby sister chromatids are paired and held together, is essential for accurate segregation of the sister chromatids and homologous chromosomes into daughter cells during mitosis and meiosis, respectively. Sister chromatid cohesion requires a cohesin complex comprised of structural maintenance of chromosome adenosine triphosphatases and accessory proteins that regulate the association of the complex with chromosomes or that are involved in the establishment or release of cohesion. The cohesin complex also plays important roles in the repair of DNA double-strand breaks, regulation of gene expression and chromosome condensation. In this review, we summarize progress in understanding cohesion dynamics in plants, with the aim of uncovering differences at specific stages. We also highlight dissimilarities between plants and other eukaryotes with respect to the key players involved in the achievement of cohesion, pointing out areas that require further study.

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Reduced activity ofArabidopsischromosome-cohesion regulator geneCTF7/ECO1alters cytosine methylation status and retrotransposon expression

Cited by 3 publications

References 23 publications

The cohesion establishment factor Esco1 acetylates α-tubulin to ensure proper spindle assembly in oocyte meiosis

The cohesion establishment factor Esco1 acetylates α-tubulin to ensure proper spindle assembly in oocyte meiosis

The Role of Structural Maintenance of Chromosomes Complexes in Meiosis and Genome Maintenance: Translating Biomedical and Model Plant Research Into Crop Breeding Opportunities

In Favor of Establishment: Regulation of Chromatid Cohesion in Plants

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