Reduced bacterial growth and increased osteoblast proliferation on titanium with a nanophase TiO&lt;sub&gt;2&lt;/sub&gt; surface treatment

Bhardwaj, Garima; Webster, Thomas J.

doi:10.2147/ijn.s116105

Cited by 41 publications

(16 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanotopographies capable of achieving both antibacterial effects and eukaryotic cell adhesion would be desirable for medical implant applications 23 – 27 . However, topographical features alone will always be limited in terms of bioactivity, since the surfaces with maximum antibacterial potential might not be favorable for the optimal osteoinductivity, or vice versa.…”

Section: Discussionmentioning

confidence: 99%

“…Although antibacterial nanotopographies with the capacity to positively stimulate eukaryotic cells have been reported 23 – 27 , the high aspect ratio nanotopographies are often not optimal for eukaryotic cell-instructive purposes (e.g. osteointegration) as they are quite different from the ones cited in the literature to promote osteogenesis 11 , 15 – 17 and could even lead to a reduction in the cell adhesive properties of the surface.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands

Fraioli

Tsimbouri

Fisher

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Engineering the interface between biomaterials and tissues is important to increase implant lifetime and avoid failures and revision surgeries. Permanent devices should enhance attachment and differentiation of stem cells, responsible for injured tissue repair, and simultaneously discourage bacterial colonization; this represents a major challenge. To take first steps towards such a multifunctional surface we propose merging topographical and biochemical cues on the surface of a clinically relevant material such as titanium. In detail, our strategy combines antibacterial nanotopographical features with integrin selective synthetic ligands that can rescue the adhesive capacity of the surfaces and instruct mesenchymal stem cell (MSC) response. To this end, a smooth substrate and two different high aspect ratio topographies have been produced and coated either with an αvβ3-selective peptidomimetic, an α5β1-selective peptidomimetic, or an RGD/PHSRN peptidic molecule. Results showed that antibacterial effects of the substrates could be maintained when tested on pathogenic Pseudomonas aeruginosa. Further, functionalization increased MSC adhesion to the surfaces and the αvβ3-selective peptidomimetic-coated nanotopographies promoted osteogenesis. Such a dual physicochemical approach to achieve multifunctional surfaces represents a first step in the design of novel cell-instructive biomaterial surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands

Fraioli

Tsimbouri

Fisher

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…These motility mechanisms of all types play an important role in virulence and colonization. Probably, the nanoscale roughness created by the incorporation of CNT was an influencing factor for the adhesion of S. aureus to the nanocomposites [27]. The use of nanocomposites as delivery or antibacterial devices implies that the material not only has to prevent bacterial attachment, but also it has to be compatible to the cells.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Nanocomposites based on pH-sensitive hydrogels and chitosan decorated carbon nanotubes with antibacterial properties

Bellingeri

Mulko

Molina

et al. 2018

Materials Science and Engineering: C

View full text Add to dashboard Cite

The present work aimed to study the properties of a novel nanocomposite with promising biomedical applications. Nanocomposites were prepared by the addition of different concentrations of chitosan decorated carbon nanotubes to acrylamide-co-acrylic acid hydrogels. The nanocomposites chemical structure was characterized by Fourier Transform Infrared Spectroscopy (FT-IR). The FT-IR shows the typical bands due to the hydrogel and additionally the peaks at 1750 cm and 1450 cm that correspond to the carbon nanotubes incorporated into the polymer matrix. Mechanical properties and swelling measurements in different buffer solutions were also performed. The nanocomposites showed improved mechanical properties and a stronger pH-response. In order to evaluate antimicrobial activity, the growth and adhesion of Staphylococcus aureus to nanocomposites were studied. Cytocompatibility was also evaluated by MTT assay on MDCK and 3T3 cell lines. The nanocomposites were found to be cytocompatible and showed a reduced bacterial colonization.

show abstract

“…For example, titanium dioxide nanotubes (TiO 2 -NTs) have been proven to be effective in promoting osteogenesis, 3 regulating immune response, 4 and killing bacteria. 5 Besides, TiO 2 -NTs are considered as a new kind of surface strategy for controlled drug delivery systems, such as silver nanoparticles (AgNPs) which can prevent infection with the broadspectrum antimicrobial property. 6 However, a considerable level of cytotoxicity will be invoked by the inappropriate therapeutic window concentration of Ag + ions, which greatly limits the clinical application.…”

Section: Introductionmentioning

confidence: 99%

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

Chen¹,

Guan²,

Ren³

et al. 2020

IJN

View full text Add to dashboard Cite

Introduction: The bone regeneration of endosseous implanted biomaterials is often impaired by the host immune response, especially macrophage-related inflammation which plays an important role in the bone healing process. Thus, it is a promising strategy to design an osteo-immunomodulatory biomaterial to take advantage of the macrophage-related immune response and improve the osseointegration performance of the implant. Methods: In this study, we developed an antibacterial silver nanoparticle-loaded TiO 2 nanotubes (Ag@TiO 2-NTs) using an electrochemical anodization method to make the surface modification and investigated the influences of Ag@TiO 2-NTs on the macrophage polarization, osteo-immune microenvironment as well as its potential molecular mechanisms in vitro and in vivo. Results: The results showed that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions had the excellent ability to induce the macrophage polarization towards the M2 phenotype and create a suitable osteo-immune microenvironment in vitro, via inhibiting PI3K/Akt, suppressing the downstream effector GLUT1, and activating autophagy. Moreover, Ag@TiO 2-NTs surface could improve bone formation, suppress inflammation, and promote osteo-immune microenvironment compared to the TiO 2-NTs and polished Ti surfaces in vivo. These findings suggested that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions could not only inhibit the inflammation process but also promote the bone healing by inducing healing-associated M2 polarization. Discussion: Using this surface modification strategy to modulate the macrophage-related immune response, rather than prevent the host response, maybe a promising strategy for implant surgeries in the future.

show abstract

Reduced bacterial growth and increased osteoblast proliferation on titanium with a nanophase TiO<sub>2</sub> surface treatment

Cited by 41 publications

References 35 publications

Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands

Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligands

Nanocomposites based on pH-sensitive hydrogels and chitosan decorated carbon nanotubes with antibacterial properties

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

Contact Info

Product

Resources

About