Reduced Coniferin and Enhanced 6-Methoxypodophyllotoxin Production in Linum flavum Cell Cultures

Abstract

a b s t r a c t treatment of cell suspension cultures of Linum flavum l. with na 2 eDta reduced the coniferin and enhanced the 6-methoxypodophyllotoxin (6-MPt) production in a concentration-dependent way, in a range of 0.1-5 mM. on day 14 after treatment with na 2 eDta, an inhibition of the coniferin production up to 88% was found. the maximum enhancement of the 6-MPt production was 400% on day 7 after treatment with 5 mM na 2 eDta. the reduction in coniferin accumulation in the suspension cultures correlated with and inhibition of coniferyl alcohol glucosyltransferase (cagt) activity as determined in cell homogenates. on day 14 after treatment with 2 and 5 mM na 2 eDta, the cagt activity was inhibited up to >89 %. the inhibitory effect of na 2 EDTA on CAGT was also shown in a partially purified enzyme preparation. Several metal ions and the elicitors nigeran and salicylic acid had no significant effect on the production of coniferin and 6-MPt.