Reduced Content of Homogalacturonan Does Not Alter the Ion-Mediated Increase in Xylem Hydraulic Conductivity in Tobacco

Nardini, Andrea; Gascó, Antonio; Cervone, Felice; Salleo, Sebastiano

doi:10.1104/pp.106.091827

Cited by 15 publications

(4 citation statements)

References 46 publications

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“…The absence of an effect of calcium removal on cavitation resistance in Salix and Betula suggests that HG might not be present in the pit membranes of these highly vulnerable species. In further support of our results, Nardini et al . (2007) did not find any differences in the ion‐mediated effect on stem hydraulic conductivity in tobacco plants with reduced HG content when compared with control plants with unaltered composition.…”

Section: Discussionsupporting

confidence: 93%

Linking irradiance‐induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation

Plavcová

Hacke

Sperry

2010

Plant Cell & Environment

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The effect of shading on xylem hydraulic traits and xylem anatomy was studied in hybrid poplar (Populus trichocarpa ¥ deltoides, clone H11-11). Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading resulted in more vulnerable and less efficient xylem. Air is thought to enter vessels through pores in inter-vessel pit membranes, thereby nucleating cavitation. Therefore, we tested if the ultrastructure and/or chemistry of pit membranes differed in SH and C plants. Transmission electron micrographs revealed that pit membranes were thinner in SH, which was paralleled by lower compound middle lamella thickness. Immunolabelling with JIM5 and JIM7 monoclonal antibodies surprisingly indicated that pectic homogalacturonans were not present in the mature pit membrane regardless of the light treatment. Porosity measurements conducted with scanning electron microscopy were significantly affected by the method used for sample dehydration. Drying through a gradual ethanol series seems to be a better alternative to drying directly from a hydrated state for pit membrane observations in poplar. Scanning electron microscopy based estimates of pit membrane porosity probably overestimated real porosity as suggested by the results from the 'rare pit' model.

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Section: Discussionsupporting

confidence: 93%

Linking irradiance‐induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation

Plavcová

Hacke

Sperry

2010

Plant Cell & Environment

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“…An exception to this is the report of pectins based on JIM7 in juvenile shoots of Vitis vinifera ( Sun et al 2011 ). Moreover, the ionic effect did not decrease in transgenic plants of Nicotiana tabacum (PG7 and PG16) with reduced HG content in comparison with wild-type plants with assumingly higher pectin levels ( Nardini et al 2007a ). If pectin is lacking in intervessel pit membranes, it is possible that other acidic cell-wall matrix polysaccharides/proteoglycans, such as heteroxylans or AGPs, could show a similar swelling and shrinking behaviour as pectins ( Li and Pan 2010 ), although there is also little evidence for their distribution in intervessel pit membranes ( Table 1 ; van Doorn et al.…”

Section: Introductionmentioning

confidence: 94%

The chemical identity of intervessel pit membranes inAcerchallenges hydrogel control of xylem hydraulic conductivity

Klepsch¹,

Schmitt²,

Knox³

et al. 2015

AoB PLANTS

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Long-distance water transport in wood is well known to be affected by the ionic concentration of xylem sap. Based on immunocytochemistry, we demonstrate that pectic polysaccharides and glycoproteins are not associated with vessel-vessel pit membranes. Therefore, we challenge the hypothesis that the ion-mediated enhancement of hydraulic conductance is caused by a swelling or shrinking of pectins surrounding pit membrane pores. Our findings reinforce the need for an alternative hypothesis besides the pit membrane chemistry to understand this so-called “ionic effect”.

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“…These excellent mutant plants can provide new and useful genetic information for further genetic improvement of L. olgensis. Tracheid, which is smaller and thicker than the conduit in angiosperms, is the only water-carrying tissue in the xylem of gymnosperms (Nardini et al, 2007). Various studies have reported that different tracheid widths and lengths can affect the water transport resistance of the xylem (Rolland et al, 2015).…”

Section: Changes In Morphological and Cytological Structures Of Mutant Plantsmentioning

confidence: 99%

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

Cai

Zhou

et al. 2021

Front. Plant Sci.

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Larix olgensis is a tall deciduous tree species that has many applications in the wood fiber industry. Bud mutations are somatic mutations in plants and are considered an ideal material to identify and describe the molecular mechanism of plant mutation. However, the molecular regulatory mechanisms of bud mutations in L. olgensis remain unknown. In this study, dwarfed (or stunted), short-leaved, and multi-branched mutants of L. olgensis were found and utilized to identify crucial genes and regulatory networks controlling the multiple branch structure of L. olgensis. The physiological data showed that the branch number, bud number, fresh and dry weight, tracheid length, tracheid length-width ratio, inner tracheid diameter, and epidermal cell area of mutant plants were higher than that of wild-type plants. Hormone concentration measurements found that auxin, gibberellin, and abscisic acid in the mutant leaves were higher than that in wild-type plants. Moreover, the transcriptome sequencing of all samples using the Illumina Hiseq sequencing platform. Transcriptome analysis identified, respectively, 632, 157, and 199 differentially expressed genes (DEGs) in buds, leaves, and stems between mutant plants and wild type. DEGs were found to be involved in cell division and differentiation, shoot apical meristem activity, plant hormone biosynthesis, and sugar metabolism. Furthermore, bZIP, WRKY, and AP2/ERF family transcription factors play a role in bud formation. This study provides new insights into the molecular mechanisms of L. olgensis bud and branch formation and establishes a fundamental understanding of the breeding of new varieties in L. olgensis.

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Reduced Content of Homogalacturonan Does Not Alter the Ion-Mediated Increase in Xylem Hydraulic Conductivity in Tobacco

Cited by 15 publications

References 46 publications

Linking irradiance‐induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation

Linking irradiance‐induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation

The chemical identity of intervessel pit membranes inAcerchallenges hydrogel control of xylem hydraulic conductivity

Insight Into the Multiple Branches Traits of a Mutant in Larix olgensis by Morphological, Cytological, and Transcriptional Analyses

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