Reduced expression of Claudin‐7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease

Sauer, Torill; Pedersen, Mette Kristin; Ebeltoft, Kristin; Næss, Oddvar

doi:10.1111/j.1365-2303.2005.00257.x

Cited by 97 publications

(87 citation statements)

References 18 publications

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“…We observe a selection for the loss of occludin and claudins-3, -4 and -7 in the liveraggressive breast cancer cell populations. These observations are consistent with a loss of claudin-1 and -7 expression in primary breast cancers of increasing grade (Kramer et al, 2000;Kominsky et al, 2003;Sauer et al, 2005). Our results are also in agreement with a recent publication demonstrating the loss of cell adhesion molecules, including claudin-4 and claudin-7, in 4T1 sub-populations that are metastatic to the liver (Erin et al, 2009).…”

Section: Discussionsupporting

confidence: 93%

“…Some studies demonstrate the loss of specific claudins during breast cancer progression, whereas others suggest that overexpression of particular claudins are associated with poor outcome in breast cancer patients (Martin and Jiang, 2009). For example, some breast cancers exhibit a loss of claudin expression, including claudin-1, -2 and -7 (Kominsky et al, 2003;Sauer et al, 2005;Tokes et al, 2005;Kim et al, 2008), whereas claudin-3 and -4 can be overexpressed in human breast cancers, particularly in the triple-negative subtype (Kominsky et al, 2004;Blanchard et al, 2009;Kulka et al, 2009;Lanigan et al, 2009). Although these studies, which are focused on claudin expression in the primary tumor, suggest that specific claudin proteins may fulfill tumor-suppressor or tumor-promoting roles in breast cancer, little is known regarding claudin expression in breast cancer metastases.…”

Section: Introductionmentioning

confidence: 99%

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Claudin-2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of integrin complexes

et al. 2010

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The liver represents the third most frequent site of metastasis in patients with breast cancer. We performed in vivo selection using 4T1 breast cancer cells to identify genes associated with the liver metastatic phenotype. Coincident with the loss of numerous tight-junctional proteins, we observe claudin-2 overexpression, specifically in liver-aggressive breast cancer cells. We further demonstrate that claudin-2 is both necessary and sufficient for the ability of 4T1 breast cancer cells to colonize and grow in the liver. The liver-aggressive breast cancer cells display a claudin-2-mediated increase in their ability to adhere to extracellular matrix (ECM) components, such as fibronectin and type IV collagen. Claudin-2 facilitates these cell/matrix interactions by increasing the cell surface expression of a 2 b 1 -and a 5 b 1 -integrin complexes in breast cancer cells. Indeed, claudin-2-mediated adhesion to fibronectin and type IV collagen can be blocked with neutralizing antibodies that target a 5 b 1 and a 2 b 1 complexes, respectively. Immunohistochemical analyses reveal that claudin-2, although weakly expressed in primary human breast cancers, is readily detected in all liver metastasis samples examined to date. Together, these results uncover novel roles for claudin-2 in promoting breast cancer adhesion to the ECM and define its importance during breast cancer metastasis to the liver.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Claudin-2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of integrin complexes

et al. 2010

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“…This hypothesis is concordant with the observation that upregulated claudin 2 decreases the tightness of TJ strands in Madin-Darby canine kidney cells, with resultant cell leakage (24). In invasive ductal carcinomas, as well as in head and neck and metastatic breast cancers, claudin 7 expression has been observed to be decreased (25)(26)(27), and lower expression levels of claudin 1, 4 and 7 have been detected in colorectal carcinoma (28).…”

Section: Discussionsupporting

confidence: 73%

Expression of claudin 1, 4 and 7 in thyroid neoplasms

et al. 2017

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“…CLDN-3 and CLDN-4 are upregulated in ovarian and uterine cancers, 10,17,35,36 and CLDN-4 expression has been exploited for therapeutic purposes. 24 CLDN-7 expression is decreased in breast carcinomas, 13,37 but Adenocarcinoma vs bronchial cell (n ¼ 6) upregulated in gastric carcinomas. 9 With regards to other tight junction membrane proteins, we observed a statistically significant decrease in JAM-1 transcript levels in squamous cell carcinoma, when compared with normal bronchial cells, and in OCLN mRNA in squamous cell carcinoma.…”

Section: Discussionmentioning

confidence: 97%

Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas

et al. 2007

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We investigated the expression of tight junction proteins in human lung squamous cell carcinomas and adenocarcinomas by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). We found a statistically significant correlation between diagnosis and positivity of tumors with either claudin (CLDN)-1 or CLDN-5. Squamous cell carcinomas and basal cells of bronchial epithelium were positive for CLDN-1 and negative for CLDN-5, whereas adenocarcinomas, normal cylindrical cells and pneumocytes were positive for CLDN-5 and negative for CLDN-1, suggesting different pathways in tumor development and progression. CLDN-4 and ZO-1 staining were detected in both types of tumors, whereas cingulin (CGN) was not detected in squamous cell carcinomas. Quantitative RT-PCR was used to evaluate changes in transcript levels for a large panel of tight junction proteins. In squamous cell carcinomas, we observed statistically significant decreases in the mRNA levels of JAM-1, occludin, CLDN-3, CLDN-4, CLDN-7, CGN, ZO-2 and ZO-3, and an increase in CLDN-1 mRNA. In adenocarcinomas, when transcript levels were compared with bronchial cells, we observed statistically significant decreases in the mRNA levels of CLDN-1, CLDN-3, CLDN-4, CLDN-7, ZO-2 and ZO-3. These results indicate that characterization of tight junction protein expression in human lung tumors can be an additional diagnostic tool and provide new insights on their histogenesis. Modern Pathology (2007) 20, 947-954;

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Reduced expression of Claudin‐7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease

Abstract: Reduced expression of Claudin-7 correlated with higher tumour grade, metastatic disease, including loco-regional recurrences and with cellular discohesion.

Cited by 97 publications

References 18 publications

Claudin-2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of integrin complexes

Claudin-2 is selectively enriched in and promotes the formation of breast cancer liver metastases through engagement of integrin complexes

Expression of claudin 1, 4 and 7 in thyroid neoplasms

Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas

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