Reduced expression of <i>Dicer</i> associated with poor prognosis in lung cancer patients

Karube, Yoko; Tanaka, Hisaaki; Osada, Hiroyuki; Tomida, Shuta; Tatematsu, Yoshio; Yanagisawa, Kiyoshi; Yatabe, Yasushi; Takamizawa, Junichi; Miyoshi, Shinichiro; Mitsudomi, Tetsuya; Takahashi, Takashi

doi:10.1111/j.1349-7006.2005.00015.x

Cited by 565 publications

(449 citation statements)

References 28 publications

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“…Micheal et al reported that mirs-143 and -145 consistently display reduced steady-state levels of the mature miRNA at the adenomatous and cancer stages of colorectal cancers and that in cancer cells decreased levels of mature miRNAs was due to reduced Dicer-processing activity. In our study, since the introduction of mir-143 or -145 precursors into the DLD-1 and SW480 cells caused growth inhibition, the activity and expression of Dicer and RNA-induced silencing complex (RISC) proteins which exert the enzymatic modification of precursor miRNAs seemed to be intact (16)(17)(18). Therefore, we propose that the cause of inadequate expression of mirs-143 and -145 was due to the perturbation of transcription and/or the enzymatic process of the transition from primary miRNAs to precursor ones including Drosha and DGCR8, events that occur in the nucleus (16,17).…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs 143 and 145 are possible common onco-microRNAs in human cancers

Akao¹,

2006

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Abstract. MicroRNAs (miRNAs) are endogenously expressed RNAs 18-24 nucleotides in length that regulate gene expression through translational repression by binding to a target mRNA. It is thought that the expression level of miRNAs, which act like antioncogenes, is frequently reduced in cancers because of chromosome deletion and epigenetic changes. Since the processing of miRNAs has been characterized to be enzymatic in nature, the expression levels of miRNAs are closely associated with the activity and levels of such enzymes. Here, we found that miRNA 143 and 145 expression levels were extremely reduced in colon cancer cells and commonly in the other kinds of cancer cells tested. The transfection of each precursor miRNA into the cells demonstrated a significant growth inhibition in human colon cancer DLD-1 and SW480 cells, and ERK5 was determined to be the target gene of miRNA 143. Since the presence of genomic loci of the miRNAs was confirmed by PCR in the cell lines and the precursor miRNAs exhibited a growth inhibitory effect in DLD-1 and SW480 cells, the early processes such as transcription and enzymatic modification from primary miRNAs to precursor miRNAs seemed to be commonly disturbed. These findings indicate that the miRNAs 143 and 145 could become good tumor markers and provide an important clue in the study of the mechanism of oncogenesis involving miRNAs.

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Section: Discussionmentioning

confidence: 99%

MicroRNAs 143 and 145 are possible common onco-microRNAs in human cancers

Akao¹,

2006

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“…11,12 Reduced expression of Dicer also has been shown to be involved in lung cancer and is associated with a reduced life span of cancer patients. 13 While the majority of miRNA genes seem to be downregulated in cancer (see below), some miRNAs tend to be upregulated. Human B cell lymphomas, for example, show elevated levels of miR-155, which is derived from the nonprotein-coding transcript of the proto-oncogene BIC.…”

Section: The Role Of Mirnas In Human Tumorigenesismentioning

confidence: 99%

Methylation of Human MicroRNA Genes in Normal and Neoplastic Cells

et al. 2007

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“…They have been found to have roles in cell growth, differentiation, apoptosis and tumourigenesis. [7][8][9][10][11][12][13][14] Furthermore, unique microRNA expression profiles have been able to classify various cancers. Recently, microRNAs were implicated in the development of ovarian cancer: 39 microRNAs are differentially regulated between tumour and normal ovarian tissue.…”

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confidence: 99%

Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma

et al. 2009

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MicroRNAs are a group of small non-coding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature microRNAs in human cancers. We characterized the alteration in expression of a select group of microRNAs in primary peritoneal carcinoma relative to matched cases of ovarian serous carcinoma. MicroRNA expression was analysed using semi-quantitative stem-loop RT-PCR on a set of 34 formalin-fixed paraffin-embedded samples. Protein expression of p53 and bcl-2 was quantified in the corresponding tissue microarray. We provide definitive evidence that there is downregulation of a select group of microRNAs in tumours meeting Gynaecological Oncology Group criteria for primary peritoneal carcinoma relative to ovarian serous carcinoma. Specifically, we show decreased p53 expression and downregulation of miR-195 and miR-497 from the microRNA cluster site at chromosome 17p13.1 in primary peritoneal carcinoma relative to ovarian serous carcinoma. miR-195 and miR-497 may have potential roles as tumour-suppressor genes in primary peritoneal tumourigenesis.

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Reduced expression of Dicer associated with poor prognosis in lung cancer patients

Cited by 565 publications

References 28 publications

MicroRNAs 143 and 145 are possible common onco-microRNAs in human cancers

MicroRNAs 143 and 145 are possible common onco-microRNAs in human cancers

Methylation of Human MicroRNA Genes in Normal and Neoplastic Cells

Potentially important microRNA cluster on chromosome 17p13.1 in primary peritoneal carcinoma

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