Reduced expression of the αβ T-cell antigen receptorby alveolar T-cells

Yamaguchi, Etsuro; Itoh, Akichika; Furuya, Ken; Hizawa, Nobuyuki; Ohnuma, N; Kodama, Natsuko; Kojima, Junichiro; Kawakami, Yoshikazu

doi:10.1034/j.1399-3003.1999.13d20.x

Cited by 5 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4). In addition, as reported previously [36] and as also confirmed in this study, a low TCR intensity was also observed in BALF T cells from the controls, thus suggesting that their low TCR intensity cannot be explained solely by TCR modulation following their activation [36]. Furthermore, a substantial proportion of BALF T cells expressed other NK cell markers and the intracellular perforin/granzyme B and the biased TCR β repertoire was observed in each T cell subset.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Characterization of bronchoalveolar lavage T cell subsets in sarcoidosis on the basis of CD57, CD4 and CD8

Kurumagawa

Seki²,

Kobayashi

et al. 2003

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYT cells expressing CD57 (a natural killer cell marker) with interferon-g (IFN-g ) producing capacity increase under various conditions. CD57 + T cells are also present in the bronchoalveolar lavage fluid (BALF) of sarcoidosis, and several phenotypical and functional analyses of these cells have been reported. In the present study, BALF T cells obtained from 52 patients with sarcoidosis were classified further into CD4 + CD57 + T cells, CD4 + CD57 -T cells, CD8 + CD57 + T cells and CD8 + CD57 -T cells and their phenotypes and functional characteristics were assessed. Substantial proportions of these T cell subsets expressed natural killer cell markers CD161 and CD122. The biased expansion of V b 2 T cells was observed in both CD4 + CD57 + T cells and CD4 + CD57 -T cells in BALF from most patients, while the expansion of other V b T cells was also observed in some patients. Unexpectedly, the biased expansion of certain V b T cells was also seen in either CD8 + CD57 + T cells or CD8 + CD57 -T cells, while the expanded V b T cells in CD8 + T cells differed substantially among individuals. BALF T cells showed a remarkably lower T cell receptor (TCR) intensity than that of peripheral blood T cells. Both CD8 + T cell subsets in BALF of sarcoidosis expressed the intracellular perforin/granzyme B, while all four subsets expressed intracellular IFN-g after in vitro activation, and CD4 + T cells, especially CD4 + CD57 + T cells, expressed tumour necrosis factor-a . These findings indicate that CD57 + T cells as well as CD57 -T cells in the BALF are phenotypically and functionally different from peripheral blood T cells and may play an important role in the Th1 dominant state and inflammation in pulmonary sarcoidosis.

show abstract

Section: Discussionsupporting

confidence: 90%

“…As reported previously in whole T cells [35,36], all T cell subsets in the BALF express extremely small numbers of TCRs on their surface (Fig. 4).…”

Section: Discussionsupporting

confidence: 79%

Characterization of bronchoalveolar lavage T cell subsets in sarcoidosis on the basis of CD57, CD4 and CD8

Kurumagawa

Seki²,

Kobayashi

et al. 2003

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…11 The expression of CD3 antigen by alveolar T cells is also likewise reduced in health and disease. 12, 13 We analysed the activation status, cytokine production and cytotoxic function of gd T cells in induced sputum compared with those found in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

T cells expressing the γδ receptor are essential for Th2‐mediated inflammation in patients with acute exacerbation of asthma

Hamzaoui

Kahan²,

Ayed³

et al. 2002

Mediators of Inflammation

View full text Add to dashboard Cite

OBJECTIVE: T lymphocytes have a central regulatory role in the pathogenesis of asthma. The objective of this study was to characterize immunologically the activation stage of asthma and the functional profile of lymphocytes from induced sputum, with particular emphasis on gammadelta T cells. METHODS: Induced sputum was collected from 10 patients with acute exacerbation of asthma, and from healthy controls. The expression of activation markers on freshly isolated induced sputum lymphocytes and T-cell subsets was analyzed by double immunofluorescent staining and flow cytometry. Fas ligand (FasL) was determined by reverse transcriptase-polymerase chain reaction analysis. The phenotype of gammadelta T-cell subpopulations was tested by A13 and BB3 monoclonal antibodies. In this context, the functional profile of gammadelta T cells was tested in a chromium releasing test. RESULTS: A significantly decreased proportion of alphabeta T cells and an increased proportion of gammadelta T cells, CD56+ cells and CD8+ gammadelta T cells were found in asthma patients compared with healthy controls. In asthmatic patients, there is a significantly increased proportion of T cells expressing CD69 and CD25 antigen. After stimulation of gammadelta T cells, an increased expression of intracellular tumour necrosis factor-alpha, interleukin (IL)-4 and IL10 cytokines were found at higher levels than controls. Interferon-gamma was observed at similar levels in asthma patients and healthy controls. Freshly isolated T-cell receptor (TCR) gammadelta+ cells exhibited an increased percentage of FasL in our patient group. FasL mRNA was detected in TCR gammadelta+ cells before and after IL2 stimulation. TCR gammadelta+ cells were cytotoxic against the K562 cell line. This natural killer activity was mediated by the A13-positive subpopulation. CONCLUSION: The presence of cytokines producing gammadelta cells in induced sputum of asthmatic patients is consistent with regulatory activities. These cells display also cytotoxic function.

show abstract

References

2021

Flow Cytometry of Hematological Malignancies

View full text Add to dashboard Cite

Reduced expression of the αβ T-cell antigen receptorby alveolar T-cells

Cited by 5 publications

References 26 publications

Characterization of bronchoalveolar lavage T cell subsets in sarcoidosis on the basis of CD57, CD4 and CD8

Characterization of bronchoalveolar lavage T cell subsets in sarcoidosis on the basis of CD57, CD4 and CD8

T cells expressing the γδ receptor are essential for Th2‐mediated inflammation in patients with acute exacerbation of asthma

References

Contact Info

Product

Resources

About