Reduced fasting plasma levels of diazepam‐binding inhibitor in adolescents with anorexia nervosa

Conti, Elisa; Tremolizzo, Lucio; Bomba, Monica; Uccellini, Orlando; Rossi, Maria Sara; Raggi, Maria Elisabetta; Neri, Francesca; Ferrarese, Carlo; Nacinovich, Renata

doi:10.1002/eat.22129

Cited by 21 publications

(29 citation statements)

References 22 publications

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“…1) was very low, implying that an extremely high number of subjects should have been recruited to demonstrate a putative difference in plasma DBI levels between patients and controls. Moreover, fasting DBI plasma levels were very similar with respect to those obtained in another series of 10 age-comparable healthy controls [20]. Concomitantly, we showed that DHEA-S is significantly increased and, since cortisol (8.00 a.m.) was not changed, the CDR was inevitably reduced in BPD patients with respect to healthy controls.…”

Section: Discussionsupporting

confidence: 59%

“…DBI plasma levels were assessed in duplicate by commercial human ELISA kits (AbFrontier; DBI capture antibody: 2F2, DBI detector antibody: biotin-27C9) and expressed in nanograms per millilitre. Intra-assay and interassay variabilities were below 10%, and the readings were all within the linear part of the standard curve, as previously shown [20]. …”

Section: Methodsmentioning

confidence: 81%

“…Blood withdrawal was always performed between 8.00 and 8.30 a.m., following overnight fasting and immediately separated as previously described [20]. DBI plasma levels were assessed in duplicate by commercial human ELISA kits (AbFrontier; DBI capture antibody: 2F2, DBI detector antibody: biotin-27C9) and expressed in nanograms per millilitre.…”

Section: Methodsmentioning

confidence: 99%

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Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Conti

Nacinovich²,

Bomba³

et al. 2013

Neuropsychobiology

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Background: Borderline personality disorder (BPD) patients display a complex and heterogeneous clinical phenotype that plausibly implies variable underlying pathogenic mechanisms. A dysregulation of peripheral benzodiazepine receptors has previously been shown in BPD peripheral tissues, implying possible alterations of its ligand, the diazepam binding inhibitor (DBI) or of the downstream products of its activation, i.e. neuroactive steroids. Methods: The aim of this work consisted in assessing, by ELISA, fasting plasma levels of DBI and dehydroepiandrosterone sulphate (DHEA-S), including cortisol and the cortisol-to-DHEA-S molar ratio (CDR), in 17 BPD adolescents versus 13 healthy controls, testing the possibility that clinical scales related to depressive or anxious traits (CDI, STAI-Y) or to disease severity (BPDCL) might be associated with a selective dysregulation of these parameters. Results: DBI plasma levels were unchanged, while DHEA-S ones were significantly increased (approx. 70%) and the CDR decreased in BPD patients. No meaningful correlations with clinical variables emerged. Conclusion: Our results indicate that a dysfunction of the neurosteroid system might be operative in BPD in spite of unchanged DBI plasma levels and that DHEA-S might represent a generalized trait marker for the altered stress response that is associated with this disorder.

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Section: Discussionsupporting

confidence: 59%

Section: Methodsmentioning

confidence: 81%

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Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Conti

Nacinovich²,

Bomba³

et al. 2013

Neuropsychobiology

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“…Laboratory investigations were carried out blindly with respect to clinical data. Leptin plasma levels were assessed by commercial human ELISA kits (DRG Diagnostics [7]). Aβ40 and Aβ42 plasma levels were determined in duplicate using a commercially available ELISA kit (Millipore) and expressed in picograms per millilitre, as previously reported [16].…”

Section: Methodsmentioning

confidence: 99%

“…In addition, Aβ and other APP fragments play a role in the physiology of synaptic transmission, neuronal migration and outgrowth [4,5], suggesting that a dysfunction of Aβ signalling might be potentially operative in those synaptopathies expressing without overt neurodegenerative phenomena, as most psychiatric diseases are thought to be [6]. AN patients, in particular, display neuropsychiatric dysfunctions beyond the core ones related with eating behaviour, including, among others, mood [7] or memory impairment [8], both involving altered connectivity within brain networks [9]. Synaptic dysfunction has never been consistently investigated in AN patients, but experimental evidence derived from animal models of activity-based anorexia supports this assumption [10].…”

Section: Introductionmentioning

confidence: 99%

Beta-Amyloid Plasma Levels in Adolescents with Anorexia Nervosa of the Restrictive Type

Conti¹,

Nacinovich²,

Bomba³

et al. 2015

Neuropsychobiology

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Background: Reduced plasma leptin and elevated homocysteine (Hcy) are known to lead to increased β-amyloid (Aβ) production, besides being hallmarks of anorexia nervosa (AN) of the restrictive type. AN subjects display several neuropsychiatric manifestations, which may entail Aβ-mediated altered synaptic functions. The aim of this study consisted in assessing Aβ plasma levels in AN patients. Methods: A total of 24 adolescent female AN outpatients were recruited together with 12 age-comparable healthy controls. For each subject we assessed Aβ40 and leptin plasma levels, as well as APOE genotype. Hcy plasma levels were also determined in AN patients who underwent clinical characterization, including the Eating Disorder Inventory-3 (EDI-3), the Children's Depression Inventory (CDI) and the estimation of the speed of BMI loss (DPI, disease progression index). Results: Plasma Aβ40 levels were similar between patients and controls, while a marked reduction was observed for leptin (∼80%) in AN patients. Aβ40 plasma levels failed to correlate with leptin, while a linear correlation was present with Hcy (r = 0.50, p < 0.03). Examined clinical features were not related with Aβ40 plasma levels, with the only exception of the DPI (r = 0.47, p < 0.03). Conclusion: This exploratory study does not support a significant role for altered Aβ production in AN-associated dysfunctions. Further studies are required to clarify whether exceptions to this conclusion can be drawn for those patients expressing significantly elevated Hcy plasma levels or for those progressing more rapidly.

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Paradoxical increase of plasma vitamin B₁₂ and folates with disease severity in anorexia nervosa

Corbetta

Tremolizzo

Conti

et al. 2014

Intl J Eating Disorders

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These data suggest that plasma levels of vitamin B12 might be an early marker of liver dysfunction, possibly also related to more severe psychopathological aspects. The identification of patients with higher fasting plasma vitamin B12 levels could therefore lead to earlier and more careful refeeding interventions. Further studies will clarify the potential role of this vitamin in AN clinical practice.

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Reduced fasting plasma levels of diazepam‐binding inhibitor in adolescents with anorexia nervosa

Abstract: These data further extend our knowledge of neuropeptide dysfunction in AN, and plasma DBI may represent a marker for this disease, in particular considering its correlation with comorbid mood disorders.

Cited by 21 publications

References 22 publications

Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Beta-Amyloid Plasma Levels in Adolescents with Anorexia Nervosa of the Restrictive Type

Paradoxical increase of plasma vitamin B₁₂ and folates with disease severity in anorexia nervosa

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Reduced fasting plasma levels of diazepam‐binding inhibitor in adolescents with anorexia nervosa

Abstract: These data further extend our knowledge of neuropeptide dysfunction in AN, and plasma DBI may represent a marker for this disease, in particular considering its correlation with comorbid mood disorders.

Cited by 21 publications

References 22 publications

Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Diazepam Binding Inhibitor and Dehydroepiandrosterone Sulphate Plasma Levels in Borderline Personality Disorder Adolescents

Beta-Amyloid Plasma Levels in Adolescents with Anorexia Nervosa of the Restrictive Type

Paradoxical increase of plasma vitamin B12 and folates with disease severity in anorexia nervosa

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Product

Resources

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Paradoxical increase of plasma vitamin B₁₂ and folates with disease severity in anorexia nervosa