Reduced graphene oxide–melamine formaldehyde as a highly efficient platform for needle trap microextraction of volatile organic compounds

There are as many measurement values of the true column hold-up volume, V0, as techniques applied to evaluate this most important property in liquid chromatography (LC). The relative errors made on V0 measurements using conventional “non-retained” markers—such as acetone, uracil, or thiourea in reversed-phase liquid chromatography (RPLC), or benzene or acenaphthene in hydrophilic interaction chromatography (HILIC)—can be as large as ±30%. This situation is extremely confusing for LC users who wish to classify and predict the retention behavior of LC columns. In this work, along with advances in mass spectrometry (MS) instrumentation, the hold-up volume of any LC column—including, but not limited to, RPLC, HILIC, ion exchange chromatography (IEX), and mixed-mode columns—is accurately measured by injecting labeled deuterated acetonitrile (CD3CN) molecules and detecting them selectively by MS-single ion reaction (m/z = 45) using non-labeled and pure acetonitrile (CH3CN) as the eluent. This proposed harmonization of all conventional V0 measurement methods is illustrated and successfully applied to RPLC, HILIC, anion exchange (AEX), and RP-AEX mixed-mode chromatography, irrespective of the mobile phase composition selected.

Section: Other Sorptive Mediamentioning

confidence: 99%

Harmonization of Experimental Methods Used to Measure the True Hold-Up Volume of Liquid Chromatography Columns

Gritti

Smith

2023

Section: Other Sorptive Mediamentioning

confidence: 99%

What Are My Options to Improve My Separation? Part I: Foundational Concepts

Stoll

2023

Many high performance liquid chromatography (HPLC) users are confronted with questions about how to improve the performance of an existing or recently developed method. These days, we have many technological options to consider (for example, using higher pressures or temperatures, or both), but how do we choose one (or a few) to try? A systematic approach to method improvement can save time and resources by using methods that are likely to be better, and more robust, than those developed using a trial-and-error approach.

Section: Other Sorptive Mediamentioning

confidence: 99%

The Rise of Automation—Evolve or Die?

Taylor¹

2023

MultiMedia Pharma Sciences, LLC. provides certain customer contact data (such as customer's name, addresses, phone numbers, and e-mail addresses) to third parties who wish to promote relevant products, services, and other opportunities that may be of interest to you. If you do not want MultiMedia Pharma Sciences, LLC. to make your contact information available to third parties for marketing purposes, simply email